Loading…
Modulation of Vasomotor Function by Perivascular Adipose Tissue of Renal Artery Depends on Severity of Arterial Dysfunction to Nitric Oxide and Severity of Metabolic Parameters
Perivascular adipose tissue (PVAT) enhances vascular relaxation of mesenteric arteries in SHRSP.Z-Leprfa/IzmDmcr rats (SPZF), a metabolic syndrome model. We investigated and compared the effects of PVAT on the renal artery in SPZF with those on SHR/NDmcr-cp rats (CP). Renal arteries with and without...
Saved in:
Published in: | Biomolecules (Basel, Switzerland) Switzerland), 2022-06, Vol.12 (7), p.870 |
---|---|
Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c451t-30a0bb5f560a7cf53c1b9e0836efb2c8d885e41fa4cef801ced7ffd89b9ad48a3 |
---|---|
cites | cdi_FETCH-LOGICAL-c451t-30a0bb5f560a7cf53c1b9e0836efb2c8d885e41fa4cef801ced7ffd89b9ad48a3 |
container_end_page | |
container_issue | 7 |
container_start_page | 870 |
container_title | Biomolecules (Basel, Switzerland) |
container_volume | 12 |
creator | Kagota, Satomi Futokoro, Risa McGuire, John J. Maruyama-Fumoto, Kana Shinozuka, Kazumasa |
description | Perivascular adipose tissue (PVAT) enhances vascular relaxation of mesenteric arteries in SHRSP.Z-Leprfa/IzmDmcr rats (SPZF), a metabolic syndrome model. We investigated and compared the effects of PVAT on the renal artery in SPZF with those on SHR/NDmcr-cp rats (CP). Renal arteries with and without PVAT were isolated from 23-week-old SPZF and CP. The effects of PVAT on acetylcholine- and nitroprusside-induced relaxation were examined using bioassays with phenylephrine-contracted arterial rings. Acetylcholine-induced relaxations without PVAT in SPZF and CP were 0.7- and 0.5-times lower in females than in males, respectively. In the presence of PVAT, acetylcholine-induced relaxations increased 1.4- and 2-times in male and female CP, respectively, but did not differ in SPZF. Nitroprusside-induced relaxation with and without PVAT was 0.7-times lower in female than in male SPZF but did not differ in CP. Angiotensin-II type-1 receptor (AT1R)/AT1R-associated protein mRNA ratios were lower in CP than in the SPZF and negatively correlated with the difference in arterial relaxation with and without PVAT. The effects of renal artery PVAT differed between the SPZF and CP groups. Higher levels of enhanced AT1R activity in SPZF PVAT may drive these differences by impairing the vascular smooth muscle responses to nitric oxide. |
doi_str_mv | 10.3390/biom12070870 |
format | article |
fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_38863f2c48084d63ac6ff50ffe769db2</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_38863f2c48084d63ac6ff50ffe769db2</doaj_id><sourcerecordid>2693908251</sourcerecordid><originalsourceid>FETCH-LOGICAL-c451t-30a0bb5f560a7cf53c1b9e0836efb2c8d885e41fa4cef801ced7ffd89b9ad48a3</originalsourceid><addsrcrecordid>eNpdkl1rFDEUhgdRbKm98wcEvPHC1XzMR-ZGWFprC60tWsW7cCY5qVlmJtsks7j_qj_R7G6VriGQcM7zPgRyiuI1o--FaOmHzvmBcdpQ2dBnxSHnTM54I34-f3I_KI5jXNC8ZN5cvCwORCWlKHl9WDxceTP1kJwfibfkB0Q_-OQDOZtGva12a3KDwa0g6gwGMjdu6SOSWxfjhJvQVxyhJ_OQMKzJKS5xNJHk5Ddc5WBab5ht12XsdB3tX3Xy5ItLwWly_dsZJDCavdAVJuh8n_s3EGDArIivihcW-ojHj-dR8f3s0-3J-ezy-vPFyfxypsuKpZmgQLuuslVNodG2Epp1LVIparQd19JIWWHJLJQaraRMo2msNbLtWjClBHFUXOy8xsNCLYMbIKyVB6e2BR_uFITkdI9KSFkLy3UpqSxNLUDX1lbUWmzq1nQ8uz7uXMupG9BoHFOAfk-63xndL3XnV6oVjMtaZsHbR0Hw9xPGpAYXNfY9jOinqHjdVrwVVUMz-uY_dOGnkD9oS-WZkbximXq3o3TwMQa0_x7DqNpMlno6WeIPzHLEzg</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2693908251</pqid></control><display><type>article</type><title>Modulation of Vasomotor Function by Perivascular Adipose Tissue of Renal Artery Depends on Severity of Arterial Dysfunction to Nitric Oxide and Severity of Metabolic Parameters</title><source>PubMed Central database</source><source>Publicly Available Content (ProQuest)</source><creator>Kagota, Satomi ; Futokoro, Risa ; McGuire, John J. ; Maruyama-Fumoto, Kana ; Shinozuka, Kazumasa</creator><creatorcontrib>Kagota, Satomi ; Futokoro, Risa ; McGuire, John J. ; Maruyama-Fumoto, Kana ; Shinozuka, Kazumasa</creatorcontrib><description>Perivascular adipose tissue (PVAT) enhances vascular relaxation of mesenteric arteries in SHRSP.Z-Leprfa/IzmDmcr rats (SPZF), a metabolic syndrome model. We investigated and compared the effects of PVAT on the renal artery in SPZF with those on SHR/NDmcr-cp rats (CP). Renal arteries with and without PVAT were isolated from 23-week-old SPZF and CP. The effects of PVAT on acetylcholine- and nitroprusside-induced relaxation were examined using bioassays with phenylephrine-contracted arterial rings. Acetylcholine-induced relaxations without PVAT in SPZF and CP were 0.7- and 0.5-times lower in females than in males, respectively. In the presence of PVAT, acetylcholine-induced relaxations increased 1.4- and 2-times in male and female CP, respectively, but did not differ in SPZF. Nitroprusside-induced relaxation with and without PVAT was 0.7-times lower in female than in male SPZF but did not differ in CP. Angiotensin-II type-1 receptor (AT1R)/AT1R-associated protein mRNA ratios were lower in CP than in the SPZF and negatively correlated with the difference in arterial relaxation with and without PVAT. The effects of renal artery PVAT differed between the SPZF and CP groups. Higher levels of enhanced AT1R activity in SPZF PVAT may drive these differences by impairing the vascular smooth muscle responses to nitric oxide.</description><identifier>ISSN: 2218-273X</identifier><identifier>EISSN: 2218-273X</identifier><identifier>DOI: 10.3390/biom12070870</identifier><identifier>PMID: 35883426</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Abdomen ; Acetylcholine ; Adipocytes ; Adipose tissue ; Age ; Angiotensin AT1 receptors ; Angiotensin II ; Blood pressure ; Body fat ; Coronary vessels ; Endothelium ; Females ; Gender differences ; Gene expression ; Glucose ; Hypertension ; Inflammation ; Kidney diseases ; Laboratory animals ; Males ; Metabolic disorders ; Metabolic syndrome ; mRNA ; Nitric oxide ; Obesity ; Phenylephrine ; Renal artery ; Sex differences ; Smooth muscle ; Veins & arteries ; Womens health</subject><ispartof>Biomolecules (Basel, Switzerland), 2022-06, Vol.12 (7), p.870</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 by the authors. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-30a0bb5f560a7cf53c1b9e0836efb2c8d885e41fa4cef801ced7ffd89b9ad48a3</citedby><cites>FETCH-LOGICAL-c451t-30a0bb5f560a7cf53c1b9e0836efb2c8d885e41fa4cef801ced7ffd89b9ad48a3</cites><orcidid>0000-0003-0302-3884 ; 0000-0003-2044-5552</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2693908251/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2693908251?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids></links><search><creatorcontrib>Kagota, Satomi</creatorcontrib><creatorcontrib>Futokoro, Risa</creatorcontrib><creatorcontrib>McGuire, John J.</creatorcontrib><creatorcontrib>Maruyama-Fumoto, Kana</creatorcontrib><creatorcontrib>Shinozuka, Kazumasa</creatorcontrib><title>Modulation of Vasomotor Function by Perivascular Adipose Tissue of Renal Artery Depends on Severity of Arterial Dysfunction to Nitric Oxide and Severity of Metabolic Parameters</title><title>Biomolecules (Basel, Switzerland)</title><description>Perivascular adipose tissue (PVAT) enhances vascular relaxation of mesenteric arteries in SHRSP.Z-Leprfa/IzmDmcr rats (SPZF), a metabolic syndrome model. We investigated and compared the effects of PVAT on the renal artery in SPZF with those on SHR/NDmcr-cp rats (CP). Renal arteries with and without PVAT were isolated from 23-week-old SPZF and CP. The effects of PVAT on acetylcholine- and nitroprusside-induced relaxation were examined using bioassays with phenylephrine-contracted arterial rings. Acetylcholine-induced relaxations without PVAT in SPZF and CP were 0.7- and 0.5-times lower in females than in males, respectively. In the presence of PVAT, acetylcholine-induced relaxations increased 1.4- and 2-times in male and female CP, respectively, but did not differ in SPZF. Nitroprusside-induced relaxation with and without PVAT was 0.7-times lower in female than in male SPZF but did not differ in CP. Angiotensin-II type-1 receptor (AT1R)/AT1R-associated protein mRNA ratios were lower in CP than in the SPZF and negatively correlated with the difference in arterial relaxation with and without PVAT. The effects of renal artery PVAT differed between the SPZF and CP groups. Higher levels of enhanced AT1R activity in SPZF PVAT may drive these differences by impairing the vascular smooth muscle responses to nitric oxide.</description><subject>Abdomen</subject><subject>Acetylcholine</subject><subject>Adipocytes</subject><subject>Adipose tissue</subject><subject>Age</subject><subject>Angiotensin AT1 receptors</subject><subject>Angiotensin II</subject><subject>Blood pressure</subject><subject>Body fat</subject><subject>Coronary vessels</subject><subject>Endothelium</subject><subject>Females</subject><subject>Gender differences</subject><subject>Gene expression</subject><subject>Glucose</subject><subject>Hypertension</subject><subject>Inflammation</subject><subject>Kidney diseases</subject><subject>Laboratory animals</subject><subject>Males</subject><subject>Metabolic disorders</subject><subject>Metabolic syndrome</subject><subject>mRNA</subject><subject>Nitric oxide</subject><subject>Obesity</subject><subject>Phenylephrine</subject><subject>Renal artery</subject><subject>Sex differences</subject><subject>Smooth muscle</subject><subject>Veins & arteries</subject><subject>Womens health</subject><issn>2218-273X</issn><issn>2218-273X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdkl1rFDEUhgdRbKm98wcEvPHC1XzMR-ZGWFprC60tWsW7cCY5qVlmJtsks7j_qj_R7G6VriGQcM7zPgRyiuI1o--FaOmHzvmBcdpQ2dBnxSHnTM54I34-f3I_KI5jXNC8ZN5cvCwORCWlKHl9WDxceTP1kJwfibfkB0Q_-OQDOZtGva12a3KDwa0g6gwGMjdu6SOSWxfjhJvQVxyhJ_OQMKzJKS5xNJHk5Ddc5WBab5ht12XsdB3tX3Xy5ItLwWly_dsZJDCavdAVJuh8n_s3EGDArIivihcW-ojHj-dR8f3s0-3J-ezy-vPFyfxypsuKpZmgQLuuslVNodG2Epp1LVIparQd19JIWWHJLJQaraRMo2msNbLtWjClBHFUXOy8xsNCLYMbIKyVB6e2BR_uFITkdI9KSFkLy3UpqSxNLUDX1lbUWmzq1nQ8uz7uXMupG9BoHFOAfk-63xndL3XnV6oVjMtaZsHbR0Hw9xPGpAYXNfY9jOinqHjdVrwVVUMz-uY_dOGnkD9oS-WZkbximXq3o3TwMQa0_x7DqNpMlno6WeIPzHLEzg</recordid><startdate>20220623</startdate><enddate>20220623</enddate><creator>Kagota, Satomi</creator><creator>Futokoro, Risa</creator><creator>McGuire, John J.</creator><creator>Maruyama-Fumoto, Kana</creator><creator>Shinozuka, Kazumasa</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-0302-3884</orcidid><orcidid>https://orcid.org/0000-0003-2044-5552</orcidid></search><sort><creationdate>20220623</creationdate><title>Modulation of Vasomotor Function by Perivascular Adipose Tissue of Renal Artery Depends on Severity of Arterial Dysfunction to Nitric Oxide and Severity of Metabolic Parameters</title><author>Kagota, Satomi ; Futokoro, Risa ; McGuire, John J. ; Maruyama-Fumoto, Kana ; Shinozuka, Kazumasa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-30a0bb5f560a7cf53c1b9e0836efb2c8d885e41fa4cef801ced7ffd89b9ad48a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Abdomen</topic><topic>Acetylcholine</topic><topic>Adipocytes</topic><topic>Adipose tissue</topic><topic>Age</topic><topic>Angiotensin AT1 receptors</topic><topic>Angiotensin II</topic><topic>Blood pressure</topic><topic>Body fat</topic><topic>Coronary vessels</topic><topic>Endothelium</topic><topic>Females</topic><topic>Gender differences</topic><topic>Gene expression</topic><topic>Glucose</topic><topic>Hypertension</topic><topic>Inflammation</topic><topic>Kidney diseases</topic><topic>Laboratory animals</topic><topic>Males</topic><topic>Metabolic disorders</topic><topic>Metabolic syndrome</topic><topic>mRNA</topic><topic>Nitric oxide</topic><topic>Obesity</topic><topic>Phenylephrine</topic><topic>Renal artery</topic><topic>Sex differences</topic><topic>Smooth muscle</topic><topic>Veins & arteries</topic><topic>Womens health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kagota, Satomi</creatorcontrib><creatorcontrib>Futokoro, Risa</creatorcontrib><creatorcontrib>McGuire, John J.</creatorcontrib><creatorcontrib>Maruyama-Fumoto, Kana</creatorcontrib><creatorcontrib>Shinozuka, Kazumasa</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Biomolecules (Basel, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kagota, Satomi</au><au>Futokoro, Risa</au><au>McGuire, John J.</au><au>Maruyama-Fumoto, Kana</au><au>Shinozuka, Kazumasa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of Vasomotor Function by Perivascular Adipose Tissue of Renal Artery Depends on Severity of Arterial Dysfunction to Nitric Oxide and Severity of Metabolic Parameters</atitle><jtitle>Biomolecules (Basel, Switzerland)</jtitle><date>2022-06-23</date><risdate>2022</risdate><volume>12</volume><issue>7</issue><spage>870</spage><pages>870-</pages><issn>2218-273X</issn><eissn>2218-273X</eissn><abstract>Perivascular adipose tissue (PVAT) enhances vascular relaxation of mesenteric arteries in SHRSP.Z-Leprfa/IzmDmcr rats (SPZF), a metabolic syndrome model. We investigated and compared the effects of PVAT on the renal artery in SPZF with those on SHR/NDmcr-cp rats (CP). Renal arteries with and without PVAT were isolated from 23-week-old SPZF and CP. The effects of PVAT on acetylcholine- and nitroprusside-induced relaxation were examined using bioassays with phenylephrine-contracted arterial rings. Acetylcholine-induced relaxations without PVAT in SPZF and CP were 0.7- and 0.5-times lower in females than in males, respectively. In the presence of PVAT, acetylcholine-induced relaxations increased 1.4- and 2-times in male and female CP, respectively, but did not differ in SPZF. Nitroprusside-induced relaxation with and without PVAT was 0.7-times lower in female than in male SPZF but did not differ in CP. Angiotensin-II type-1 receptor (AT1R)/AT1R-associated protein mRNA ratios were lower in CP than in the SPZF and negatively correlated with the difference in arterial relaxation with and without PVAT. The effects of renal artery PVAT differed between the SPZF and CP groups. Higher levels of enhanced AT1R activity in SPZF PVAT may drive these differences by impairing the vascular smooth muscle responses to nitric oxide.</abstract><cop>Basel</cop><pub>MDPI AG</pub><pmid>35883426</pmid><doi>10.3390/biom12070870</doi><orcidid>https://orcid.org/0000-0003-0302-3884</orcidid><orcidid>https://orcid.org/0000-0003-2044-5552</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 2218-273X |
ispartof | Biomolecules (Basel, Switzerland), 2022-06, Vol.12 (7), p.870 |
issn | 2218-273X 2218-273X |
language | eng |
recordid | cdi_doaj_primary_oai_doaj_org_article_38863f2c48084d63ac6ff50ffe769db2 |
source | PubMed Central database; Publicly Available Content (ProQuest) |
subjects | Abdomen Acetylcholine Adipocytes Adipose tissue Age Angiotensin AT1 receptors Angiotensin II Blood pressure Body fat Coronary vessels Endothelium Females Gender differences Gene expression Glucose Hypertension Inflammation Kidney diseases Laboratory animals Males Metabolic disorders Metabolic syndrome mRNA Nitric oxide Obesity Phenylephrine Renal artery Sex differences Smooth muscle Veins & arteries Womens health |
title | Modulation of Vasomotor Function by Perivascular Adipose Tissue of Renal Artery Depends on Severity of Arterial Dysfunction to Nitric Oxide and Severity of Metabolic Parameters |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T03%3A19%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Modulation%20of%20Vasomotor%20Function%20by%20Perivascular%20Adipose%20Tissue%20of%20Renal%20Artery%20Depends%20on%20Severity%20of%20Arterial%20Dysfunction%20to%20Nitric%20Oxide%20and%20Severity%20of%20Metabolic%20Parameters&rft.jtitle=Biomolecules%20(Basel,%20Switzerland)&rft.au=Kagota,%20Satomi&rft.date=2022-06-23&rft.volume=12&rft.issue=7&rft.spage=870&rft.pages=870-&rft.issn=2218-273X&rft.eissn=2218-273X&rft_id=info:doi/10.3390/biom12070870&rft_dat=%3Cproquest_doaj_%3E2693908251%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c451t-30a0bb5f560a7cf53c1b9e0836efb2c8d885e41fa4cef801ced7ffd89b9ad48a3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2693908251&rft_id=info:pmid/35883426&rfr_iscdi=true |