Differentially Expressed microRNAs in Peritoneal Dialysis Effluent-Derived Exosomes from the Patients with Ultrafiltration Failure

Background. Ultrafiltration failure remains one of the most severe complications of long-term peritoneal dialysis (PD), which results in death. This study aimed to characterize the circulating exosomal microRNA (miRNA) profiles associated with ultrafiltration failure and explore its underlying mecha...

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Bibliographic Details
Published in:Genetics Research 2022-08, Vol.2022, p.1-12
Main Authors: Wu, Weifei, Wu, Xu, Cheng, Zhiqun, Yang, Zhenzhen, Lu, Minhui, Cheng, Jing
Format: Article
Language:English
Subjects:
Antibodies
Biomarkers
Complications
Creatinine
Dialysate
Dialysis
Effluents
End-stage renal disease
Exosomes
Failure
Gene sequencing
Glucose
Kidney diseases
MicroRNAs
miRNA
Nanoparticles
Peritoneal dialysis
Peritoneum
Peritonitis
Proteolysis
Rap1 protein
Ribonucleic acid
RNA
Therapeutic targets
Transmission electron microscopy
Ubiquitin
Ultracentrifugation
Ultrafiltration
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Summary:Background. Ultrafiltration failure remains one of the most severe complications of long-term peritoneal dialysis (PD), which results in death. This study aimed to characterize the circulating exosomal microRNA (miRNA) profiles associated with ultrafiltration failure and explore its underlying mechanisms. Methods. Exosomes were isolated from the peritoneal dialysis effluent (PDE) of patients with ultrafiltration failure or success using the ultracentrifugation method, and then transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and western blot were used for exosome characterization. After that, the isolated exosomes were sent for small RNA sequencing, and eight differentially expressed miRNAs (DE-miRNAs) were chosen for RT-qPCR validation. Results. TEM, NTA, and western blot revealed that exosomes were successfully isolated. After sequencing, 70 DE-miRNAs involved in ultrafiltration were identified, including 41 upregulated ones and 29 downregulated ones. Functional analyses revealed that these DE-miRNAs were significantly enriched in pathways of cancer, ubiquitin-mediated proteolysis, axon orientation, and the Rap1 and Ras signaling pathways. In addition, the consistency rate of RT-qPCR and sequencing results was 75%, which indicated the relatively high reliability of the sequencing data. Conclusions. Our findings implied that these DE-miRNAs may be potential biomarkers of ultrafiltration failure, which would help us to discover novel therapeutic targets/pathways for ultrafiltration failure in patients with end-stage renal disease.
ISSN:1469-5073
0016-6723
1469-5073
DOI:10.1155/2022/2276175