Synthesis and Characterization of Ciprofloxacin-loaded Chitosan Nanoparticles for Eradication of Pseudomonas aeroginosa Biofilm

• Published in: Pharmaceutical and biomedical research 2023-09, Vol.9 (3), p.243-258
• Main Authors: Faridi, Foroogh, Bahador, Nima, Shoja, Saeed, Abbasi, Sahar
• Format: Article
• Language: English
• Subjects: biofilm; chitosan; nanoparticle; pseudomonas aeruginosa
• ISSN: 2423-4486; 2423-4494
• DOI: 10.32598/PBR.9.3.1112.1

Background: Pseudomonas aeruginosa biofilm is one of the problems in antibiotic treatment of infections. Nanomedicines, such as chitosan (CS) can carry multiple drugs and improve the therapeutic effects of antibiotics. Objectives: This study aimed at the synthesis and characterization of ciprofloxacin-loaded chitosan nanoparticles for eradication of P. aeroginosa biofilm. Methods: Cipro-CS microparticles were prepared by ionic gelation method and their size, zeta potential, and drug release pattern were determined. MBEC and MBIC of different groups of antibiotics (ciprofloxacin, ciprofloxacin-PAβN, CS ciprofloxacin, and CS ciprofloxacin-PAβN) were performed on biofilm samples of P. aeroginosa. Results: Ciprofloxacin loading efficiency was 35.51%, and encapsulation efficiency was 55.06%. Released ciprofloxacin from CS nanoparticles was 80% after 24 hours. Biofilm production was positive in 96.7% of the isolates while 30.1% of the samples had strong biofilm. The best result for MBIC was CS ciprofloxacin, CS ciprofloxacin-PAβN, ciprofloxacin- PAβN, and ciprofloxacin, respectively. For MBEC the result was slightly different and from the best to better CS Ciprofloxacin-PAβN, CS Ciprofloxacin, Ciprofloxacin-PAβN, and ciprofloxacin. Conclusion: Today, with increasing antibiotic resistance, there are many challenges in treating infections. Due to the role of biofilm in antibiotic resistance, researchers are looking for new antibiotics to treat infections.