Therapeutic Role of Annona muricata Fruit and Bee Venom Against MNU-Induced Breast Cancer in Pregnant Rats and its Complications on the Ovaries

• Published in: Breast cancer targets and therapy 2021-01, Vol.13, p.431-445
• Main Authors: El-Beltagy, Abd El-Fattah B M, Elsyyad, Hassan I H, Abdelaziz, Karoline K, Madany, Amira S, Elghazaly, Mohamed M
• Format: Article
• Language: English
• Subjects: Annona muricata; Antibodies; Antioxidants; Apoptosis; bee venom; Body weight; Breast cancer; Cancer therapies; Caspase-3; Complications and side effects; Females; graviola; Hyperplasia; Inflammation; Laboratories; Males; Medical prognosis; Medical research; Microscopy; Natural products; NF-κB protein; Offspring; Original Research; Ovaries; Pregnancy; Radiation; Tumor markers; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Vagina; Venom; Weaning; Womens health
• ISSN: 1179-1314; 1179-1314
• DOI: 10.2147/BCTT.S306971

To evaluate the potential therapeutic role of (graviola) fruit and bee venom (BV) against -methylnitrosourea (MNU)-induced breast cancer in pregnant female rats and complications in the ovaries. A total of 24 female rats were induced with a single dose of MNU (50 mg/kg body weight). After confirmation of positive tumor marker, female rats were placed with the males for mating. The pregnant rats were randomly divided into four groups (n=6): MNU-induced only (group 1), MNU-induced rats and supplemented with 200 mg/kg diet (group 2), MNU-induced and treated with two doses of BV 75 μg/kg (group 3), and MNU-induced and treated with both and BV (group 4). In group 1, the breast tissue of mothers revealed pronounced cellular hyperplasia and histopathological signs. Also, the ovarian tissue of mothers and their offspring displayed deleterious histological changes. In groups 2 and 4, histopathological signs and cellular hyperplasia markedly disappeared in breast tissue. However, the histopathological signs induced by MNU in the ovarian tissue reversed to normal in groups 2-4. Also in groups 2-4, levels of serum MMP1, NFκB, and TNFα significantly decreased, and serum caspase 3 significantly increased either in mother rats or their offspring compared to the MNU-alone group. Levels of serum MDA significantly decreased; however, levels of serum antioxidants (CAT and SOD) significantly increased in all groups 2-4 compared to MNU-alone group. has a more powerful therapeutic role than BV against MNU-induced breast cancer in rats; however, both have a powerful ameliorative role against ovarian histopathological alterations induced by MNU. Such ameliorative effects of and BV are mainly attributed to their antioxidant, anti-inflammatory, and antiproliferative constituents.