Apolipoprotein Signature of HDL and LDL from Atherosclerotic Patients in Relation with Carotid Plaque Typology: A Preliminary Report

In the past years, it has become increasingly clear that the protein cargo of the different lipoprotein classes is largely responsible for carrying out their various functions, also in relation to pathological conditions, including atherosclerosis. Accordingly, detailed information about their apoli...

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Bibliographic Details
Published in:Biomedicines 2021-09, Vol.9 (9), p.1156
Main Authors: Finamore, Francesco, Nieddu, Gabriele, Rocchiccioli, Silvia, Spirito, Rita, Guarino, Anna, Formato, Marilena, Lepedda, Antonio Junior
Format: Article
Language:English
Subjects:
Apolipoproteins
Arteriosclerosis
Atherogenesis
Atherosclerosis
Cardiovascular disease
Cholesterol
Degeneration
High density lipoprotein
Inflammation
Laboratories
Lipoproteins
lipoproteomics
Low density lipoprotein
plaque instability
Plaques
Plasma
Proteins
Proteomes
Proteomics
Surgery
Typology
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Summary:In the past years, it has become increasingly clear that the protein cargo of the different lipoprotein classes is largely responsible for carrying out their various functions, also in relation to pathological conditions, including atherosclerosis. Accordingly, detailed information about their apolipoprotein composition and structure may contribute to the revelation of their role in atherogenesis and the understanding of the mechanisms that lead to atherosclerotic degeneration and toward vulnerable plaque formation. With this aim, shotgun proteomics was applied to identify the apolipoprotein signatures of both high-density and low-density lipoproteins (HDL and LDL) plasma fractions purified from healthy volunteers and atherosclerotic patients with different plaque typologies who underwent carotid endarterectomy. By this approach, two proteins with potential implications in inflammatory, immune, and hemostatic pathways, namely, integrin beta-2 (P05107) and secretoglobin family 3A member 2 (Q96PL1), have been confirmed to belong to the HDL proteome. Similarly, the list of LDL-associated proteins has been enriched with 21 proteins involved in complement and coagulation cascades and the acute-phase response, which potentially double the protein species of LDL cargo. Moreover, differential expression analysis has shown protein signatures specific for patients with “hard” or “soft” plaques.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines9091156