Assessment of the Inhibitory Effect of Rifampicin on Amyloid Formation of Hen Egg White Lysozyme: Thioflavin T Fluorescence Assay versus FTIR Difference Spectroscopy

The inhibitory effect of rifampicin on the amyloid formation of hen egg white lysozyme was assessed with both Thioflavin T (ThT) fluorescence assay and Fourier transform infrared (FTIR) difference spectroscopy. We reveal that ThT fluorescence assay gives a false positive result due to rifampicin int...

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Bibliographic Details
Published in:Journal of spectroscopy (Hindawi) 2014-01, Vol.2014 (2014), p.1-5
Main Authors: Hu, Xiaoqian, Zeng, Xiaodan, Guo, Jianhua, Zhang, Hong, Ma, Gang, Hao, Wenying
Format: Article
Language:English
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Summary:The inhibitory effect of rifampicin on the amyloid formation of hen egg white lysozyme was assessed with both Thioflavin T (ThT) fluorescence assay and Fourier transform infrared (FTIR) difference spectroscopy. We reveal that ThT fluorescence assay gives a false positive result due to rifampicin interference, while FTIR difference spectroscopy provides a reliable assessment. With FTIR, we show that rifampicin only has marginally inhibitory effect. We then propose that FTIR difference spectroscopy can potentially be a convenient method for inhibitor screening in amyloid study.
ISSN:2314-4920
2314-4939
DOI:10.1155/2014/285806