Comparative genomics of Helicobacter pylori isolates recovered from ulcer disease patients in England

Genomic diversity of H. pylori from many different human populations is largely unknown. We compared genomes of 65 H. pylori strains from Nottingham, England. Molecular analysis was carried out to identify rearrangements within and outside the cag-pathogenicity-island (cag PAI) and DNA sequence dive...

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Bibliographic Details
Published in:BMC microbiology 2005-05, Vol.5 (1), p.32-32, Article 32
Main Authors: Kauser, Farhana, Hussain, M Abid, Ahmed, Irshad, Srinivas, Sriramula, Devi, S Manjulata, Majeed, Ahmed A, Rao, K Rajender, Khan, Aleem A, Sechi, Leonardo A, Ahmed, Niyaz
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
England - epidemiology
Genetic Variation
Genome, Bacterial
Genomics
Helicobacter Infections - epidemiology
Helicobacter Infections - microbiology
Helicobacter pylori
Helicobacter pylori - genetics
Helicobacter pylori - isolation & purification
Humans
Phylogeny
Stomach Ulcer - epidemiology
Stomach Ulcer - microbiology
Virulence Factors
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Summary:Genomic diversity of H. pylori from many different human populations is largely unknown. We compared genomes of 65 H. pylori strains from Nottingham, England. Molecular analysis was carried out to identify rearrangements within and outside the cag-pathogenicity-island (cag PAI) and DNA sequence divergence in candidate genes. Phylogenetic analysis was carried out based on various high-resolution genotyping techniques. Analyses of virulence genes (cagT, cagE, cagA, vacA, iceA, oipA and babB) revealed that H. pylori strains from England are genetically distinct from strains obtained from other countries. The toxigenic vacA s1m1 genotype was found to be less common and the plasticity region cluster was found to be disrupted in all the isolates. English isolates showed a predominance of iceA1 alleles and a functional proinflammatory oipA gene. The English H. pylori gene pool revealed several Asian/oriental features. This included the predominance of cagA - glr (cagA right junction) motif types III and II (up to 42%), presence of vacA m1c alleles and phylogenetic affinity towards East Asian / Amerindian gene pools based on fluorescent amplified fragment length polymorphism (FAFLP) analysis and glmM sequence analysis. Overall, our results demonstrated genetic affinities of H. pylori in England with both European and the Asian gene pools and some distinctive genetic features of virulence genes that may have evolved in this important European population.
ISSN:1471-2180
1471-2180
DOI:10.1186/1471-2180-5-32