Loading…
Effects of a True Prophylactic Treatment on Hippocampal and Amygdala Synaptic Plasticity and Gene Expression in a Rodent Chronic Stress Model of Social Defeat
Post-traumatic stress disorder (PTSD) is a complex stress-related disorder induced by exposure to traumatic stress that is characterized by symptoms of re-experiencing, avoidance, and hyper-arousal. While it is widely accepted that brain regions involved in emotional regulation and memory-e.g., the...
Saved in:
Published in: | International journal of molecular sciences 2023-07, Vol.24 (13), p.11193 |
---|---|
Main Authors: | , , , , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c549t-2b1b4af5c0e77d93bab2c65e6c8b4f92e923c6e495907b4d7ccb6c0b5f2383a13 |
---|---|
cites | cdi_FETCH-LOGICAL-c549t-2b1b4af5c0e77d93bab2c65e6c8b4f92e923c6e495907b4d7ccb6c0b5f2383a13 |
container_end_page | |
container_issue | 13 |
container_start_page | 11193 |
container_title | International journal of molecular sciences |
container_volume | 24 |
creator | Winzenried, Eric T Everett, Anna C Saito, Erin R Miller, Roxanne M Johnson, Taylor Neal, Eliza Boyce, Zachary Smith, Calvin Jensen, Chloe Kimball, Spencer Brantley, Adam Melendez, Gabriel Moffat, Devin Davis, Erin Aponik, Lyndsey Crofts, Tyler Dabney, Bryson Edwards, Jeffrey G |
description | Post-traumatic stress disorder (PTSD) is a complex stress-related disorder induced by exposure to traumatic stress that is characterized by symptoms of re-experiencing, avoidance, and hyper-arousal. While it is widely accepted that brain regions involved in emotional regulation and memory-e.g., the amygdala and hippocampus-are dysregulated in PTSD, the pathophysiology of the disorder is not well defined and therefore, pharmacological interventions are extremely limited. Because stress hormones norepinephrine and cortisol (corticosterone in rats) are heavily implicated in the disorder, we explored whether preemptively and systemically antagonizing β-adrenergic and glucocorticoid receptors with propranolol and mifepristone are sufficient to mitigate pathological changes in synaptic plasticity, gene expression, and anxiety induced by a modified social defeat (SD) stress protocol. Young adult, male Sprague Dawley rats were initially pre-screened for anxiety. The rats were then exposed to SD and chronic light stress to induce anxiety-like symptoms. Drug-treated rats were administered propranolol and mifepristone injections prior to and continuing throughout SD stress. Using competitive ELISAs on plasma, field electrophysiology at CA1 of the ventral hippocampus (VH) and the basolateral amygdala (BLA), quantitative RT-PCR, and behavior assays, we demonstrate that our SD stress increased anxiety-like behavior, elevated long-term potentiation (LTP) in the VH and BLA, and altered the expression of mineralocorticoid, glucocorticoid, and glutamate receptors. These measures largely reverted to control levels with the administration of propranolol and mifepristone. Our findings indicate that SD stress increases LTP in the VH and BLA and that prophylactic treatment with propranolol and mifepristone may have the potential in mitigating these and other stress-induced effects. |
doi_str_mv | 10.3390/ijms241311193 |
format | article |
fullrecord | <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_3937a283fe85467a8d2974abc826ea38</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A758317823</galeid><doaj_id>oai_doaj_org_article_3937a283fe85467a8d2974abc826ea38</doaj_id><sourcerecordid>A758317823</sourcerecordid><originalsourceid>FETCH-LOGICAL-c549t-2b1b4af5c0e77d93bab2c65e6c8b4f92e923c6e495907b4d7ccb6c0b5f2383a13</originalsourceid><addsrcrecordid>eNptkk1v1DAQhiMEomXhyBVF4sJli78SJye0ape2UhEVW87WxBnvepXYwc4i9s_wW3Hasuoi5IOtmXee12NPlr2l5Izzmny02z4yQTmltObPslMqGJsTUsrnT84n2asYt4Qwzor6ZXbCpRAll_Q0-700BvUYc29yyO_CDvPb4IfNvgM9Wp0iCGOPbsy9y6_sMHgN_QBdDq7NF_1-3UIH-WrvYJjktx3EtNtxfy-4RIf58tcQMEabANYlk2--nXjnm-BdKlmNUzb_kqLddIuV1zbxL9Ak59fZCwNdxDeP-yz7_nl5d341v_l6eX2-uJnrQtTjnDW0EWAKTVDKtuYNNEyXBZa6aoSpGdaM6xJFXdRENqKVWjelJk1hGK84UD7Lrh-4rYetGoLtIeyVB6vuAz6sFYTUWIeK11wCq7jBqhClhKpltRTQ6IqVCAk3yz49sIZd02OrU7MBuiPoccbZjVr7n4oSLlhVskT48EgI_scO46h6GzV2HTj0u6iSe8UEkdVk9v4f6dbvgktvNalKwUuefvqgWkPqwDrjk7GeoGohi4pTWTGeVGf_UaXVYm-1d2hsih8VzB8KdPAxBjSHJilR03Sqo-lM-ndPX-ag_juO_A8nG-Cc</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2836436346</pqid></control><display><type>article</type><title>Effects of a True Prophylactic Treatment on Hippocampal and Amygdala Synaptic Plasticity and Gene Expression in a Rodent Chronic Stress Model of Social Defeat</title><source>PubMed Central</source><source>ProQuest Publicly Available Content database</source><creator>Winzenried, Eric T ; Everett, Anna C ; Saito, Erin R ; Miller, Roxanne M ; Johnson, Taylor ; Neal, Eliza ; Boyce, Zachary ; Smith, Calvin ; Jensen, Chloe ; Kimball, Spencer ; Brantley, Adam ; Melendez, Gabriel ; Moffat, Devin ; Davis, Erin ; Aponik, Lyndsey ; Crofts, Tyler ; Dabney, Bryson ; Edwards, Jeffrey G</creator><creatorcontrib>Winzenried, Eric T ; Everett, Anna C ; Saito, Erin R ; Miller, Roxanne M ; Johnson, Taylor ; Neal, Eliza ; Boyce, Zachary ; Smith, Calvin ; Jensen, Chloe ; Kimball, Spencer ; Brantley, Adam ; Melendez, Gabriel ; Moffat, Devin ; Davis, Erin ; Aponik, Lyndsey ; Crofts, Tyler ; Dabney, Bryson ; Edwards, Jeffrey G</creatorcontrib><description>Post-traumatic stress disorder (PTSD) is a complex stress-related disorder induced by exposure to traumatic stress that is characterized by symptoms of re-experiencing, avoidance, and hyper-arousal. While it is widely accepted that brain regions involved in emotional regulation and memory-e.g., the amygdala and hippocampus-are dysregulated in PTSD, the pathophysiology of the disorder is not well defined and therefore, pharmacological interventions are extremely limited. Because stress hormones norepinephrine and cortisol (corticosterone in rats) are heavily implicated in the disorder, we explored whether preemptively and systemically antagonizing β-adrenergic and glucocorticoid receptors with propranolol and mifepristone are sufficient to mitigate pathological changes in synaptic plasticity, gene expression, and anxiety induced by a modified social defeat (SD) stress protocol. Young adult, male Sprague Dawley rats were initially pre-screened for anxiety. The rats were then exposed to SD and chronic light stress to induce anxiety-like symptoms. Drug-treated rats were administered propranolol and mifepristone injections prior to and continuing throughout SD stress. Using competitive ELISAs on plasma, field electrophysiology at CA1 of the ventral hippocampus (VH) and the basolateral amygdala (BLA), quantitative RT-PCR, and behavior assays, we demonstrate that our SD stress increased anxiety-like behavior, elevated long-term potentiation (LTP) in the VH and BLA, and altered the expression of mineralocorticoid, glucocorticoid, and glutamate receptors. These measures largely reverted to control levels with the administration of propranolol and mifepristone. Our findings indicate that SD stress increases LTP in the VH and BLA and that prophylactic treatment with propranolol and mifepristone may have the potential in mitigating these and other stress-induced effects.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms241311193</identifier><identifier>PMID: 37446371</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Adrenergic receptors ; Amygdala ; Amygdala - metabolism ; Animals ; Anxiety ; Arousal ; Behavior ; Corticosterone ; Disease prevention ; Electrophysiology ; Females ; Gene Expression ; Genes ; Glucocorticoid receptors ; Glucocorticoids ; Glutamic acid receptors ; Hippocampal plasticity ; Hippocampus ; Hippocampus - metabolism ; Hormones ; Long-term potentiation ; LTP ; Male ; Males ; Medicine, Preventive ; Mental disorders ; Mifepristone ; Mifepristone - pharmacology ; Neuronal Plasticity ; Neurophysiology ; Norepinephrine ; Physiological aspects ; Post traumatic stress disorder ; Preventive health services ; Propranolol ; Propranolol - pharmacology ; Propranolol hydrochloride ; Psychological stress ; PTSD ; rat ; Rats ; Rats, Sprague-Dawley ; Rodentia ; Rodents ; Social Defeat ; Social interactions ; Stress, Psychological - complications ; Synaptic plasticity ; Young adults</subject><ispartof>International journal of molecular sciences, 2023-07, Vol.24 (13), p.11193</ispartof><rights>COPYRIGHT 2023 MDPI AG</rights><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 by the authors. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c549t-2b1b4af5c0e77d93bab2c65e6c8b4f92e923c6e495907b4d7ccb6c0b5f2383a13</citedby><cites>FETCH-LOGICAL-c549t-2b1b4af5c0e77d93bab2c65e6c8b4f92e923c6e495907b4d7ccb6c0b5f2383a13</cites><orcidid>0000-0001-7298-1889 ; 0000-0003-1649-4722 ; 0000-0002-3174-0605 ; 0009-0001-2787-5822 ; 0000-0002-8987-3443 ; 0000-0002-8862-6969 ; 0000-0003-4603-0320 ; 0000-0002-2881-0772</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2836436346/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2836436346?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37446371$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Winzenried, Eric T</creatorcontrib><creatorcontrib>Everett, Anna C</creatorcontrib><creatorcontrib>Saito, Erin R</creatorcontrib><creatorcontrib>Miller, Roxanne M</creatorcontrib><creatorcontrib>Johnson, Taylor</creatorcontrib><creatorcontrib>Neal, Eliza</creatorcontrib><creatorcontrib>Boyce, Zachary</creatorcontrib><creatorcontrib>Smith, Calvin</creatorcontrib><creatorcontrib>Jensen, Chloe</creatorcontrib><creatorcontrib>Kimball, Spencer</creatorcontrib><creatorcontrib>Brantley, Adam</creatorcontrib><creatorcontrib>Melendez, Gabriel</creatorcontrib><creatorcontrib>Moffat, Devin</creatorcontrib><creatorcontrib>Davis, Erin</creatorcontrib><creatorcontrib>Aponik, Lyndsey</creatorcontrib><creatorcontrib>Crofts, Tyler</creatorcontrib><creatorcontrib>Dabney, Bryson</creatorcontrib><creatorcontrib>Edwards, Jeffrey G</creatorcontrib><title>Effects of a True Prophylactic Treatment on Hippocampal and Amygdala Synaptic Plasticity and Gene Expression in a Rodent Chronic Stress Model of Social Defeat</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Post-traumatic stress disorder (PTSD) is a complex stress-related disorder induced by exposure to traumatic stress that is characterized by symptoms of re-experiencing, avoidance, and hyper-arousal. While it is widely accepted that brain regions involved in emotional regulation and memory-e.g., the amygdala and hippocampus-are dysregulated in PTSD, the pathophysiology of the disorder is not well defined and therefore, pharmacological interventions are extremely limited. Because stress hormones norepinephrine and cortisol (corticosterone in rats) are heavily implicated in the disorder, we explored whether preemptively and systemically antagonizing β-adrenergic and glucocorticoid receptors with propranolol and mifepristone are sufficient to mitigate pathological changes in synaptic plasticity, gene expression, and anxiety induced by a modified social defeat (SD) stress protocol. Young adult, male Sprague Dawley rats were initially pre-screened for anxiety. The rats were then exposed to SD and chronic light stress to induce anxiety-like symptoms. Drug-treated rats were administered propranolol and mifepristone injections prior to and continuing throughout SD stress. Using competitive ELISAs on plasma, field electrophysiology at CA1 of the ventral hippocampus (VH) and the basolateral amygdala (BLA), quantitative RT-PCR, and behavior assays, we demonstrate that our SD stress increased anxiety-like behavior, elevated long-term potentiation (LTP) in the VH and BLA, and altered the expression of mineralocorticoid, glucocorticoid, and glutamate receptors. These measures largely reverted to control levels with the administration of propranolol and mifepristone. Our findings indicate that SD stress increases LTP in the VH and BLA and that prophylactic treatment with propranolol and mifepristone may have the potential in mitigating these and other stress-induced effects.</description><subject>Adrenergic receptors</subject><subject>Amygdala</subject><subject>Amygdala - metabolism</subject><subject>Animals</subject><subject>Anxiety</subject><subject>Arousal</subject><subject>Behavior</subject><subject>Corticosterone</subject><subject>Disease prevention</subject><subject>Electrophysiology</subject><subject>Females</subject><subject>Gene Expression</subject><subject>Genes</subject><subject>Glucocorticoid receptors</subject><subject>Glucocorticoids</subject><subject>Glutamic acid receptors</subject><subject>Hippocampal plasticity</subject><subject>Hippocampus</subject><subject>Hippocampus - metabolism</subject><subject>Hormones</subject><subject>Long-term potentiation</subject><subject>LTP</subject><subject>Male</subject><subject>Males</subject><subject>Medicine, Preventive</subject><subject>Mental disorders</subject><subject>Mifepristone</subject><subject>Mifepristone - pharmacology</subject><subject>Neuronal Plasticity</subject><subject>Neurophysiology</subject><subject>Norepinephrine</subject><subject>Physiological aspects</subject><subject>Post traumatic stress disorder</subject><subject>Preventive health services</subject><subject>Propranolol</subject><subject>Propranolol - pharmacology</subject><subject>Propranolol hydrochloride</subject><subject>Psychological stress</subject><subject>PTSD</subject><subject>rat</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rodentia</subject><subject>Rodents</subject><subject>Social Defeat</subject><subject>Social interactions</subject><subject>Stress, Psychological - complications</subject><subject>Synaptic plasticity</subject><subject>Young adults</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptkk1v1DAQhiMEomXhyBVF4sJli78SJye0ape2UhEVW87WxBnvepXYwc4i9s_wW3Hasuoi5IOtmXee12NPlr2l5Izzmny02z4yQTmltObPslMqGJsTUsrnT84n2asYt4Qwzor6ZXbCpRAll_Q0-700BvUYc29yyO_CDvPb4IfNvgM9Wp0iCGOPbsy9y6_sMHgN_QBdDq7NF_1-3UIH-WrvYJjktx3EtNtxfy-4RIf58tcQMEabANYlk2--nXjnm-BdKlmNUzb_kqLddIuV1zbxL9Ak59fZCwNdxDeP-yz7_nl5d341v_l6eX2-uJnrQtTjnDW0EWAKTVDKtuYNNEyXBZa6aoSpGdaM6xJFXdRENqKVWjelJk1hGK84UD7Lrh-4rYetGoLtIeyVB6vuAz6sFYTUWIeK11wCq7jBqhClhKpltRTQ6IqVCAk3yz49sIZd02OrU7MBuiPoccbZjVr7n4oSLlhVskT48EgI_scO46h6GzV2HTj0u6iSe8UEkdVk9v4f6dbvgktvNalKwUuefvqgWkPqwDrjk7GeoGohi4pTWTGeVGf_UaXVYm-1d2hsih8VzB8KdPAxBjSHJilR03Sqo-lM-ndPX-ag_juO_A8nG-Cc</recordid><startdate>20230701</startdate><enddate>20230701</enddate><creator>Winzenried, Eric T</creator><creator>Everett, Anna C</creator><creator>Saito, Erin R</creator><creator>Miller, Roxanne M</creator><creator>Johnson, Taylor</creator><creator>Neal, Eliza</creator><creator>Boyce, Zachary</creator><creator>Smith, Calvin</creator><creator>Jensen, Chloe</creator><creator>Kimball, Spencer</creator><creator>Brantley, Adam</creator><creator>Melendez, Gabriel</creator><creator>Moffat, Devin</creator><creator>Davis, Erin</creator><creator>Aponik, Lyndsey</creator><creator>Crofts, Tyler</creator><creator>Dabney, Bryson</creator><creator>Edwards, Jeffrey G</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-7298-1889</orcidid><orcidid>https://orcid.org/0000-0003-1649-4722</orcidid><orcidid>https://orcid.org/0000-0002-3174-0605</orcidid><orcidid>https://orcid.org/0009-0001-2787-5822</orcidid><orcidid>https://orcid.org/0000-0002-8987-3443</orcidid><orcidid>https://orcid.org/0000-0002-8862-6969</orcidid><orcidid>https://orcid.org/0000-0003-4603-0320</orcidid><orcidid>https://orcid.org/0000-0002-2881-0772</orcidid></search><sort><creationdate>20230701</creationdate><title>Effects of a True Prophylactic Treatment on Hippocampal and Amygdala Synaptic Plasticity and Gene Expression in a Rodent Chronic Stress Model of Social Defeat</title><author>Winzenried, Eric T ; Everett, Anna C ; Saito, Erin R ; Miller, Roxanne M ; Johnson, Taylor ; Neal, Eliza ; Boyce, Zachary ; Smith, Calvin ; Jensen, Chloe ; Kimball, Spencer ; Brantley, Adam ; Melendez, Gabriel ; Moffat, Devin ; Davis, Erin ; Aponik, Lyndsey ; Crofts, Tyler ; Dabney, Bryson ; Edwards, Jeffrey G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c549t-2b1b4af5c0e77d93bab2c65e6c8b4f92e923c6e495907b4d7ccb6c0b5f2383a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Adrenergic receptors</topic><topic>Amygdala</topic><topic>Amygdala - metabolism</topic><topic>Animals</topic><topic>Anxiety</topic><topic>Arousal</topic><topic>Behavior</topic><topic>Corticosterone</topic><topic>Disease prevention</topic><topic>Electrophysiology</topic><topic>Females</topic><topic>Gene Expression</topic><topic>Genes</topic><topic>Glucocorticoid receptors</topic><topic>Glucocorticoids</topic><topic>Glutamic acid receptors</topic><topic>Hippocampal plasticity</topic><topic>Hippocampus</topic><topic>Hippocampus - metabolism</topic><topic>Hormones</topic><topic>Long-term potentiation</topic><topic>LTP</topic><topic>Male</topic><topic>Males</topic><topic>Medicine, Preventive</topic><topic>Mental disorders</topic><topic>Mifepristone</topic><topic>Mifepristone - pharmacology</topic><topic>Neuronal Plasticity</topic><topic>Neurophysiology</topic><topic>Norepinephrine</topic><topic>Physiological aspects</topic><topic>Post traumatic stress disorder</topic><topic>Preventive health services</topic><topic>Propranolol</topic><topic>Propranolol - pharmacology</topic><topic>Propranolol hydrochloride</topic><topic>Psychological stress</topic><topic>PTSD</topic><topic>rat</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Rodentia</topic><topic>Rodents</topic><topic>Social Defeat</topic><topic>Social interactions</topic><topic>Stress, Psychological - complications</topic><topic>Synaptic plasticity</topic><topic>Young adults</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Winzenried, Eric T</creatorcontrib><creatorcontrib>Everett, Anna C</creatorcontrib><creatorcontrib>Saito, Erin R</creatorcontrib><creatorcontrib>Miller, Roxanne M</creatorcontrib><creatorcontrib>Johnson, Taylor</creatorcontrib><creatorcontrib>Neal, Eliza</creatorcontrib><creatorcontrib>Boyce, Zachary</creatorcontrib><creatorcontrib>Smith, Calvin</creatorcontrib><creatorcontrib>Jensen, Chloe</creatorcontrib><creatorcontrib>Kimball, Spencer</creatorcontrib><creatorcontrib>Brantley, Adam</creatorcontrib><creatorcontrib>Melendez, Gabriel</creatorcontrib><creatorcontrib>Moffat, Devin</creatorcontrib><creatorcontrib>Davis, Erin</creatorcontrib><creatorcontrib>Aponik, Lyndsey</creatorcontrib><creatorcontrib>Crofts, Tyler</creatorcontrib><creatorcontrib>Dabney, Bryson</creatorcontrib><creatorcontrib>Edwards, Jeffrey G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest research library</collection><collection>Research Library (Corporate)</collection><collection>ProQuest Publicly Available Content database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Winzenried, Eric T</au><au>Everett, Anna C</au><au>Saito, Erin R</au><au>Miller, Roxanne M</au><au>Johnson, Taylor</au><au>Neal, Eliza</au><au>Boyce, Zachary</au><au>Smith, Calvin</au><au>Jensen, Chloe</au><au>Kimball, Spencer</au><au>Brantley, Adam</au><au>Melendez, Gabriel</au><au>Moffat, Devin</au><au>Davis, Erin</au><au>Aponik, Lyndsey</au><au>Crofts, Tyler</au><au>Dabney, Bryson</au><au>Edwards, Jeffrey G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of a True Prophylactic Treatment on Hippocampal and Amygdala Synaptic Plasticity and Gene Expression in a Rodent Chronic Stress Model of Social Defeat</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2023-07-01</date><risdate>2023</risdate><volume>24</volume><issue>13</issue><spage>11193</spage><pages>11193-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Post-traumatic stress disorder (PTSD) is a complex stress-related disorder induced by exposure to traumatic stress that is characterized by symptoms of re-experiencing, avoidance, and hyper-arousal. While it is widely accepted that brain regions involved in emotional regulation and memory-e.g., the amygdala and hippocampus-are dysregulated in PTSD, the pathophysiology of the disorder is not well defined and therefore, pharmacological interventions are extremely limited. Because stress hormones norepinephrine and cortisol (corticosterone in rats) are heavily implicated in the disorder, we explored whether preemptively and systemically antagonizing β-adrenergic and glucocorticoid receptors with propranolol and mifepristone are sufficient to mitigate pathological changes in synaptic plasticity, gene expression, and anxiety induced by a modified social defeat (SD) stress protocol. Young adult, male Sprague Dawley rats were initially pre-screened for anxiety. The rats were then exposed to SD and chronic light stress to induce anxiety-like symptoms. Drug-treated rats were administered propranolol and mifepristone injections prior to and continuing throughout SD stress. Using competitive ELISAs on plasma, field electrophysiology at CA1 of the ventral hippocampus (VH) and the basolateral amygdala (BLA), quantitative RT-PCR, and behavior assays, we demonstrate that our SD stress increased anxiety-like behavior, elevated long-term potentiation (LTP) in the VH and BLA, and altered the expression of mineralocorticoid, glucocorticoid, and glutamate receptors. These measures largely reverted to control levels with the administration of propranolol and mifepristone. Our findings indicate that SD stress increases LTP in the VH and BLA and that prophylactic treatment with propranolol and mifepristone may have the potential in mitigating these and other stress-induced effects.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>37446371</pmid><doi>10.3390/ijms241311193</doi><orcidid>https://orcid.org/0000-0001-7298-1889</orcidid><orcidid>https://orcid.org/0000-0003-1649-4722</orcidid><orcidid>https://orcid.org/0000-0002-3174-0605</orcidid><orcidid>https://orcid.org/0009-0001-2787-5822</orcidid><orcidid>https://orcid.org/0000-0002-8987-3443</orcidid><orcidid>https://orcid.org/0000-0002-8862-6969</orcidid><orcidid>https://orcid.org/0000-0003-4603-0320</orcidid><orcidid>https://orcid.org/0000-0002-2881-0772</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1422-0067 |
ispartof | International journal of molecular sciences, 2023-07, Vol.24 (13), p.11193 |
issn | 1422-0067 1661-6596 1422-0067 |
language | eng |
recordid | cdi_doaj_primary_oai_doaj_org_article_3937a283fe85467a8d2974abc826ea38 |
source | PubMed Central; ProQuest Publicly Available Content database |
subjects | Adrenergic receptors Amygdala Amygdala - metabolism Animals Anxiety Arousal Behavior Corticosterone Disease prevention Electrophysiology Females Gene Expression Genes Glucocorticoid receptors Glucocorticoids Glutamic acid receptors Hippocampal plasticity Hippocampus Hippocampus - metabolism Hormones Long-term potentiation LTP Male Males Medicine, Preventive Mental disorders Mifepristone Mifepristone - pharmacology Neuronal Plasticity Neurophysiology Norepinephrine Physiological aspects Post traumatic stress disorder Preventive health services Propranolol Propranolol - pharmacology Propranolol hydrochloride Psychological stress PTSD rat Rats Rats, Sprague-Dawley Rodentia Rodents Social Defeat Social interactions Stress, Psychological - complications Synaptic plasticity Young adults |
title | Effects of a True Prophylactic Treatment on Hippocampal and Amygdala Synaptic Plasticity and Gene Expression in a Rodent Chronic Stress Model of Social Defeat |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T06%3A24%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20a%20True%20Prophylactic%20Treatment%20on%20Hippocampal%20and%20Amygdala%20Synaptic%20Plasticity%20and%20Gene%20Expression%20in%20a%20Rodent%20Chronic%20Stress%20Model%20of%20Social%20Defeat&rft.jtitle=International%20journal%20of%20molecular%20sciences&rft.au=Winzenried,%20Eric%20T&rft.date=2023-07-01&rft.volume=24&rft.issue=13&rft.spage=11193&rft.pages=11193-&rft.issn=1422-0067&rft.eissn=1422-0067&rft_id=info:doi/10.3390/ijms241311193&rft_dat=%3Cgale_doaj_%3EA758317823%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c549t-2b1b4af5c0e77d93bab2c65e6c8b4f92e923c6e495907b4d7ccb6c0b5f2383a13%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2836436346&rft_id=info:pmid/37446371&rft_galeid=A758317823&rfr_iscdi=true |