Bronchodilator Secondary Metabolites from Rhazya stricta Decne Aerial Parts

The plant kingdom comprises medicinally useful plants that have provided many new drugs used to treat various diseases. In our search for bronchodilator secondary metabolites from plants growing in Saudi Arabia, the total extract of Rhazya stricta showed activity against carbamylcholine- (CCh) induc...

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Bibliographic Details
Published in:Separations 2022-12, Vol.9 (12), p.412
Main Authors: Abdel-Kader, Maged S., Rehman, Najeeb U., Aldosari, Abdullah F., Almutib, Fahad S., Al Muwinea, Ali I., Saeedan, Abdulaziz S.
Format: Article
Language:English
Subjects:
alkaloids
Antimicrobial agents
bronchodilator
Calibration
Cancer
Chromatography
Ethanol
Ethyl acetate
Experiments
Glycosides
guinea pig trachea
Guinea pigs
iridoids
Laboratory animals
Liquid-liquid extraction
Medical research
Metabolites
Muscles
Pharmacy
Rhazya stricta
Smooth muscle
structure elucidation
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Summary:The plant kingdom comprises medicinally useful plants that have provided many new drugs used to treat various diseases. In our search for bronchodilator secondary metabolites from plants growing in Saudi Arabia, the total extract of Rhazya stricta showed activity against carbamylcholine- (CCh) induced bronchoconstriction in guinea pig tracheal muscles used as an ex vivo model. The fractions obtained from liquid–liquid extraction process were tested for bronchodilator effects. The most active ethyl acetate fraction (RS-E) and aqueous fraction (RS-H) were subjected to biologically guided phytochemical study using different stationary phases and chromatographic techniques to isolate the pure secondary metabolites. Five known compounds were isolated from the active fractions. Three alkaloids namely; (-)-quebrachamine (1), (+)-eburenine (2), (+)-stemmadenine (3) as well as the two iridoid glycosides loganic acid (4) and loganine (5) were identified by various spectroscopic methods. Among the isolated compounds 1 and 5 were the only active as bronchodilators in the plant. It is worth to mention that iridoid glycosides are isolated for the first time from R. stricta.
ISSN:2297-8739
2297-8739
DOI:10.3390/separations9120412