BnaA03.MKK5-BnaA06.MPK3/BnaC03.MPK3 Module Positively Contributes to Sclerotinia sclerotiorum Resistance in Brassica napus

(oilseed rape) is one of the most important oil crops worldwide, but its growth is seriously threatened by . The mechanism of oilseed rape response to this pathogen has rarely been studied. Here, it was identified that BnaA03.MKK5 whose expression was induced by infection was involved in plant immun...

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Bibliographic Details
Published in:Plants (Basel) 2022-02, Vol.11 (5), p.609
Main Authors: Zhang, Ka, Zhuo, Chenjian, Wang, Zhixin, Liu, Fei, Wen, Jing, Yi, Bin, Shen, Jinxiong, Ma, Chaozhi, Fu, Tingdong, Tu, Jinxing
Format: Article
Language:English
Subjects:
Abiotic stress
Amino acids
Apoptosis
Biosynthesis
Brassica
Brassica napus
CRISPR
Gene expression
Genes
Genetic modification
Genetic transformation
Genome editing
Genomes
Immune system
Immunoprecipitation
Infections
Kinases
mitogen-activated protein kinase
Modules
molecular mechanism
Oilseed crops
Oilseeds
Pathogens
Phosphorylation
plant defense
Plant diseases
Plant immunity
Rape plants
Rapeseed
Sclerotinia sclerotiorum
Signal transduction
Signs and symptoms
Substrates
Transgenic plants
Yeasts
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Summary:(oilseed rape) is one of the most important oil crops worldwide, but its growth is seriously threatened by . The mechanism of oilseed rape response to this pathogen has rarely been studied. Here, it was identified that BnaA03.MKK5 whose expression was induced by infection was involved in plant immunity. overexpression lines exhibited decreased disease symptoms compared to wild-type plants, accompanied by the increased expression of camalexin-biosynthesis-related genes, including and . In addition, two copies of ( and ) were induced by incubation, and BnaA03.MKK5 interacted with BnaA06.MPK3/BnaC03.MPK3 in yeast. These interactions were confirmed using in vivo co-immunoprecipitation assays. In vitro phosphorylation assays showed that BnaA06.MPK3 and BnaC03.MPK3 were the direct phosphorylation substrates of BnaA03.MKK5. The transgenic oilseed rape plants including and overexpression lines and gene editing lines mediated by CRISPR/Cas9 were generated; the results of the genetic transformation of indicate that BnMPK3 also has a positive role in resistance. This study provides information about the potential mechanism of defense against mediated by a detailed BnaA03.MKK5-BnaA06.MPK3/BnaC03.MPK3 module.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants11050609