"Miswak" Based Green Synthesis of Silver Nanoparticles: Evaluation and Comparison of Their Microbicidal Activities with the Chemical Synthesis

• Published in: Molecules (Basel, Switzerland) Switzerland), 2016-11, Vol.21 (11), p.1478-1478
• Main Authors: Shaik, Mohammed Rafi, Albalawi, Ghadeer H, Khan, Shams Tabrez, Khan, Merajuddin, Adil, Syed Farooq, Kuniyil, Mufsir, Al-Warthan, Abdulrahman, Siddiqui, Mohammed Rafiq H, Alkhathlan, Hamad Z, Khan, Mujeeb
• Format: Article
• Language: English
• Subjects: Anti-Infective Agents - chemistry; Anti-Infective Agents - pharmacology; anti-microbial activity; Chemical synthesis; Fourier transforms; Gram-Negative Bacteria - drug effects; Gram-Positive Bacteria - drug effects; Green chemistry; Green Chemistry Technology - methods; Metal Nanoparticles - chemistry; Microbial Sensitivity Tests; Microorganisms; Morphology; Nanoparticles; Natural products; Particle Size; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Roots - chemistry; Reducing Agents - chemistry; Reducing Agents - pharmacology; Salvadora persica; Salvadoraceae - chemistry; Silver; Silver - chemistry; Silver - pharmacology; silver nanoparticles; Solubility; Spectroscopy, Fourier Transform Infrared
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules21111478

Microbicidal potential of silver nanoparticles (Ag-NPs) can be drastically improved by improving their solubility or wettability in the aqueous medium. In the present study, we report the synthesis of both green and chemical synthesis of Ag-NPs, and evaluate the effect of the dispersion qualities of as-prepared Ag-NPs from both methods on their antimicrobial activities. The green synthesis of Ag-NPs is carried out by using an aqueous solution of readily available L. root extract (RE) as a bioreductant. The formation of highly crystalline Ag-NPs was established by various analytical and microscopic techniques. The rich phenolic contents of L. RE (Miswak) not only promoted the reduction and formation of NPs but they also facilitated the stabilization of the Ag-NPs, which was established by Fourier transform infrared spectroscopy (FT-IR) analysis. Furthermore, the influence of the volume of the RE on the size and the dispersion qualities of the NPs was also evaluated. It was revealed that with increasing the volume of RE the size of the NPs was deteriorated, whereas at lower concentrations of RE smaller size and less aggregated NPs were obtained. During this study, the antimicrobial activities of both chemically and green synthesized Ag-NPs, along with the aqueous RE of L., were evaluated against various microorganisms. It was observed that the green synthesized Ag-NPs exhibit comparable or slightly higher antibacterial activities than the chemically obtained Ag-NPs.