Identification of novel biomarkers for varicocele using iTRAQ LC-MS/MS technology

Differentially expressed proteins were screened between VC and normal rats, and the function of differential proteins was classified based on gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. In order to certify the differential proteins, enzyme-link...

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Bibliographic Details
Published in:Chinese medical journal 2024-02, Vol.137 (3), p.371-372
Main Authors: Lu, Xianfeng, Li, Na, Li, Lufang, Wu, Yongai, Lyu, Xuefeng, Cao, Yingli, Liu, Jianrong, Qin, Qin
Format: Article
Language:English
Subjects:
Biomarkers
Chromatography, Liquid
Correspondence
Hospitals
Humans
Infertility
Liquid Chromatography-Mass Spectrometry
Male
Mass spectrometry
Proteins
Scientific imaging
Signal transduction
Spermatogenesis
Tandem Mass Spectrometry
Testes
Varicocele - diagnosis
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Summary:Differentially expressed proteins were screened between VC and normal rats, and the function of differential proteins was classified based on gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. In order to certify the differential proteins, enzyme-linked immunology assay (ELISA) was used to detect the differential proteins in the human seminal plasmas from 30 infertile patients with VC (VC group) and 30 age-matched normal controls (control group) who were recruited from the Department of Reproductive Medicine, Shanxi Provincial People's Hospital from October to December 2019; all participants shared similar demographic characteristics in age, BMI, and endocrine level [Supplementary Table 1, http://links.lww.com/CM9/B733]. According to literature research, two down-regulated proteins of ATPase and Cu2+-transporting alpha (ATP7A) and calcium and integrin-binding protein 1 (CIB1) were screened due to the correlation with spermatogenesis.
ISSN:0366-6999
2542-5641
DOI:10.1097/CM9.0000000000002798