NMR Analyses of the Enzymatic Degradation End-Products of Diabolican: The Secreted EPS of Vibrio diabolicus CNCM I-1629

Diabolican, or HE800, is an exopolysaccharide secreted by the non-pathogenic Gram-negative marine bacterium (CNCM I-1629). This polysaccharide was enzymatically degraded by the WH2 hyaluronan lyase. The end products were purified by size-exclusion chromatography and their structures were analyzed in...

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Bibliographic Details
Published in:Marine drugs 2022-11, Vol.20 (12), p.731
Main Authors: Drouillard, Sophie, Poulet, Laurent, Boisset, Claire, Delbarre-Ladrat, Christine, Helbert, William
Format: Article
Language:English
Subjects:
Bacteria
Bacterial Proteins - chemistry
Bacteroides - enzymology
Carbon
Chromatography
Communication
diabolican
Enzymes
exopolysaccharide
Exopolysaccharides
Glycosaminoglycans
hyaluronan lyase
Hyaluronic acid
Life Sciences
Molecular structure
Molecular weight
NMR
Nuclear magnetic resonance
Nuclear Magnetic Resonance, Biomolecular
Oligosaccharides
Oligosaccharides - chemistry
Pathogens
Polysaccharide-Lyases - chemistry
Polysaccharides
Polysaccharides, Bacterial - chemistry
Size exclusion chromatography
Substrates
Vibrio - chemistry
Vibrio diabolicus
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Summary:Diabolican, or HE800, is an exopolysaccharide secreted by the non-pathogenic Gram-negative marine bacterium (CNCM I-1629). This polysaccharide was enzymatically degraded by the WH2 hyaluronan lyase. The end products were purified by size-exclusion chromatography and their structures were analyzed in depth by nuclear magnetic resonance (NMR). The oligosaccharide structures confirmed the possible site of cleavage of the enzyme showing plasticity in the substrate recognitions. The production of glycosaminoglycan-mimetic oligosaccharides of defined molecular weight and structure opens new perspectives in the valorization of the marine polysaccharide diabolican.
ISSN:1660-3397
1660-3397
DOI:10.3390/md20120731