Loading…
Molecular Survey of Vector-Borne Pathogens of Dogs and Cats in Two Regions of Saudi Arabia
Dogs and cats play an important role as reservoirs of vector-borne pathogens, yet reports of canine and feline vector-borne diseases in Saudi Arabia are scarce. Blood samples were collected from 188 free-roaming dogs and cats in Asir (70 dogs and 44 cats) and Riyadh (74 dogs), Saudi Arabia. The pres...
Saved in:
Published in: | Pathogens (Basel) 2020-12, Vol.10 (1), p.25 |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c493t-20bf34358c435960da5c9c176f851c2adbbaee0fcee19fd64ad601ba290645363 |
---|---|
cites | cdi_FETCH-LOGICAL-c493t-20bf34358c435960da5c9c176f851c2adbbaee0fcee19fd64ad601ba290645363 |
container_end_page | |
container_issue | 1 |
container_start_page | 25 |
container_title | Pathogens (Basel) |
container_volume | 10 |
creator | Alanazi, Abdullah D Alouffi, Abdulaziz S Alyousif, Mohamed S Alshahrani, Mohammad Y Abdullah, Hend H A M Abdel-Shafy, Sobhy Calvani, Nichola Eliza Davies Ansari-Lari, Maryam Sazmand, Alireza Otranto, Domenico |
description | Dogs and cats play an important role as reservoirs of vector-borne pathogens, yet reports of canine and feline vector-borne diseases in Saudi Arabia are scarce. Blood samples were collected from 188 free-roaming dogs and cats in Asir (70 dogs and 44 cats) and Riyadh (74 dogs), Saudi Arabia. The presence of
spp.,
spp., hemotropic
spp.,
spp., and
spp. was detected using a multiplex tandem real-time PCR. PCR-positive samples were further examined with specific conventional and real-time PCR followed by sequencing. Dogs from Riyadh tested negative for all pathogens, while 46 out of 70 dogs (65.7%) and 17 out of 44 cats (38.6%) from Asir were positive for at least one pathogen. Positive dogs were infected with
(57.1%),
(30%),
(15.7%), and
(1.4%), and cats were infected with
(13.6%),
Mycoplasma haemominutum (13.6%),
(9.2%), and
(2.27%), all of which are reported for the first time in Saudi Arabia. Co-infection with
and
was detected in 17 dogs (24.28%), while coinfections were not detected in cats. These results suggest that effective control and public awareness strategies for minimizing infection in animals are necessary. |
doi_str_mv | 10.3390/pathogens10010025 |
format | article |
fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_39e7a57212c14034901a57cddad99dfa</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_39e7a57212c14034901a57cddad99dfa</doaj_id><sourcerecordid>2475019625</sourcerecordid><originalsourceid>FETCH-LOGICAL-c493t-20bf34358c435960da5c9c176f851c2adbbaee0fcee19fd64ad601ba290645363</originalsourceid><addsrcrecordid>eNplkk1v1DAQhi1ERattfwAXZIkLl4A_k_iCVJYClYpAtHDgYk3sSZpVNl7spFX_Pd5uW7VgWbbH887jjxlCXnL2VkrD3m1gugwdjokzlrvQz8iBYFVZsJpXzx-t98lRSiuWW8229guyLzOhVIYfkN9fw4BuHiDS8zle4Q0NLf2Fbgqx-BDiiPT7_Tlbz8fQJQqjp0uYEu1HenEd6A_s-rDzn8Pse3ocoenhkOy1MCQ8upsX5Oenk4vll-Ls2-fT5fFZ4ZSRUyFY00olde3yYErmQTvjeFW2teZOgG8aQGStQ-Sm9aUCXzLegDCsVFqWckFOd1wfYGU3sV9DvLEBenu7EWJnIU69G9BKgxXoSnDhuGJSGcaz6bwHb4xvIbPe71ibuVmjdzhOEYYn0Keesb-0XbiyVS2k0CoD3twBYvgzY5rsuk8OhwFGDHOyQlVaMaZzBhbk9T_SVZjjmL_qVsW4KYXOKr5TuRhSitg-XIYzuy0E-18h5JhXj1_xEHGfdvkXLgev8A</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2475019625</pqid></control><display><type>article</type><title>Molecular Survey of Vector-Borne Pathogens of Dogs and Cats in Two Regions of Saudi Arabia</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Alanazi, Abdullah D ; Alouffi, Abdulaziz S ; Alyousif, Mohamed S ; Alshahrani, Mohammad Y ; Abdullah, Hend H A M ; Abdel-Shafy, Sobhy ; Calvani, Nichola Eliza Davies ; Ansari-Lari, Maryam ; Sazmand, Alireza ; Otranto, Domenico</creator><creatorcontrib>Alanazi, Abdullah D ; Alouffi, Abdulaziz S ; Alyousif, Mohamed S ; Alshahrani, Mohammad Y ; Abdullah, Hend H A M ; Abdel-Shafy, Sobhy ; Calvani, Nichola Eliza Davies ; Ansari-Lari, Maryam ; Sazmand, Alireza ; Otranto, Domenico</creatorcontrib><description>Dogs and cats play an important role as reservoirs of vector-borne pathogens, yet reports of canine and feline vector-borne diseases in Saudi Arabia are scarce. Blood samples were collected from 188 free-roaming dogs and cats in Asir (70 dogs and 44 cats) and Riyadh (74 dogs), Saudi Arabia. The presence of
spp.,
spp., hemotropic
spp.,
spp., and
spp. was detected using a multiplex tandem real-time PCR. PCR-positive samples were further examined with specific conventional and real-time PCR followed by sequencing. Dogs from Riyadh tested negative for all pathogens, while 46 out of 70 dogs (65.7%) and 17 out of 44 cats (38.6%) from Asir were positive for at least one pathogen. Positive dogs were infected with
(57.1%),
(30%),
(15.7%), and
(1.4%), and cats were infected with
(13.6%),
Mycoplasma haemominutum (13.6%),
(9.2%), and
(2.27%), all of which are reported for the first time in Saudi Arabia. Co-infection with
and
was detected in 17 dogs (24.28%), while coinfections were not detected in cats. These results suggest that effective control and public awareness strategies for minimizing infection in animals are necessary.</description><identifier>ISSN: 2076-0817</identifier><identifier>EISSN: 2076-0817</identifier><identifier>DOI: 10.3390/pathogens10010025</identifier><identifier>PMID: 33396491</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Anaplasma ; Babesia ; Bartonella ; Cats ; Deoxyribonucleic acid ; DNA ; Dogs ; Genetic testing ; Humidity ; Infections ; Infectious diseases ; Mycoplasma ; one health ; Parasites ; Pathogens ; Public awareness ; Public health ; Real time ; Saudi Arabia ; Vector-borne diseases ; Vectors (Biology) ; Zoonoses</subject><ispartof>Pathogens (Basel), 2020-12, Vol.10 (1), p.25</ispartof><rights>2021. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 by the authors. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-20bf34358c435960da5c9c176f851c2adbbaee0fcee19fd64ad601ba290645363</citedby><cites>FETCH-LOGICAL-c493t-20bf34358c435960da5c9c176f851c2adbbaee0fcee19fd64ad601ba290645363</cites><orcidid>0000-0002-4862-7668 ; 0000-0002-8450-2993 ; 0000-0002-7518-476X ; 0000-0001-6462-585X ; 0000-0001-8863-3870 ; 0000-0002-7096-0221 ; 0000-0002-8787-6755</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2475019625/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2475019625?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33396491$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alanazi, Abdullah D</creatorcontrib><creatorcontrib>Alouffi, Abdulaziz S</creatorcontrib><creatorcontrib>Alyousif, Mohamed S</creatorcontrib><creatorcontrib>Alshahrani, Mohammad Y</creatorcontrib><creatorcontrib>Abdullah, Hend H A M</creatorcontrib><creatorcontrib>Abdel-Shafy, Sobhy</creatorcontrib><creatorcontrib>Calvani, Nichola Eliza Davies</creatorcontrib><creatorcontrib>Ansari-Lari, Maryam</creatorcontrib><creatorcontrib>Sazmand, Alireza</creatorcontrib><creatorcontrib>Otranto, Domenico</creatorcontrib><title>Molecular Survey of Vector-Borne Pathogens of Dogs and Cats in Two Regions of Saudi Arabia</title><title>Pathogens (Basel)</title><addtitle>Pathogens</addtitle><description>Dogs and cats play an important role as reservoirs of vector-borne pathogens, yet reports of canine and feline vector-borne diseases in Saudi Arabia are scarce. Blood samples were collected from 188 free-roaming dogs and cats in Asir (70 dogs and 44 cats) and Riyadh (74 dogs), Saudi Arabia. The presence of
spp.,
spp., hemotropic
spp.,
spp., and
spp. was detected using a multiplex tandem real-time PCR. PCR-positive samples were further examined with specific conventional and real-time PCR followed by sequencing. Dogs from Riyadh tested negative for all pathogens, while 46 out of 70 dogs (65.7%) and 17 out of 44 cats (38.6%) from Asir were positive for at least one pathogen. Positive dogs were infected with
(57.1%),
(30%),
(15.7%), and
(1.4%), and cats were infected with
(13.6%),
Mycoplasma haemominutum (13.6%),
(9.2%), and
(2.27%), all of which are reported for the first time in Saudi Arabia. Co-infection with
and
was detected in 17 dogs (24.28%), while coinfections were not detected in cats. These results suggest that effective control and public awareness strategies for minimizing infection in animals are necessary.</description><subject>Anaplasma</subject><subject>Babesia</subject><subject>Bartonella</subject><subject>Cats</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Dogs</subject><subject>Genetic testing</subject><subject>Humidity</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Mycoplasma</subject><subject>one health</subject><subject>Parasites</subject><subject>Pathogens</subject><subject>Public awareness</subject><subject>Public health</subject><subject>Real time</subject><subject>Saudi Arabia</subject><subject>Vector-borne diseases</subject><subject>Vectors (Biology)</subject><subject>Zoonoses</subject><issn>2076-0817</issn><issn>2076-0817</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNplkk1v1DAQhi1ERattfwAXZIkLl4A_k_iCVJYClYpAtHDgYk3sSZpVNl7spFX_Pd5uW7VgWbbH887jjxlCXnL2VkrD3m1gugwdjokzlrvQz8iBYFVZsJpXzx-t98lRSiuWW8229guyLzOhVIYfkN9fw4BuHiDS8zle4Q0NLf2Fbgqx-BDiiPT7_Tlbz8fQJQqjp0uYEu1HenEd6A_s-rDzn8Pse3ocoenhkOy1MCQ8upsX5Oenk4vll-Ls2-fT5fFZ4ZSRUyFY00olde3yYErmQTvjeFW2teZOgG8aQGStQ-Sm9aUCXzLegDCsVFqWckFOd1wfYGU3sV9DvLEBenu7EWJnIU69G9BKgxXoSnDhuGJSGcaz6bwHb4xvIbPe71ibuVmjdzhOEYYn0Keesb-0XbiyVS2k0CoD3twBYvgzY5rsuk8OhwFGDHOyQlVaMaZzBhbk9T_SVZjjmL_qVsW4KYXOKr5TuRhSitg-XIYzuy0E-18h5JhXj1_xEHGfdvkXLgev8A</recordid><startdate>20201231</startdate><enddate>20201231</enddate><creator>Alanazi, Abdullah D</creator><creator>Alouffi, Abdulaziz S</creator><creator>Alyousif, Mohamed S</creator><creator>Alshahrani, Mohammad Y</creator><creator>Abdullah, Hend H A M</creator><creator>Abdel-Shafy, Sobhy</creator><creator>Calvani, Nichola Eliza Davies</creator><creator>Ansari-Lari, Maryam</creator><creator>Sazmand, Alireza</creator><creator>Otranto, Domenico</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-4862-7668</orcidid><orcidid>https://orcid.org/0000-0002-8450-2993</orcidid><orcidid>https://orcid.org/0000-0002-7518-476X</orcidid><orcidid>https://orcid.org/0000-0001-6462-585X</orcidid><orcidid>https://orcid.org/0000-0001-8863-3870</orcidid><orcidid>https://orcid.org/0000-0002-7096-0221</orcidid><orcidid>https://orcid.org/0000-0002-8787-6755</orcidid></search><sort><creationdate>20201231</creationdate><title>Molecular Survey of Vector-Borne Pathogens of Dogs and Cats in Two Regions of Saudi Arabia</title><author>Alanazi, Abdullah D ; Alouffi, Abdulaziz S ; Alyousif, Mohamed S ; Alshahrani, Mohammad Y ; Abdullah, Hend H A M ; Abdel-Shafy, Sobhy ; Calvani, Nichola Eliza Davies ; Ansari-Lari, Maryam ; Sazmand, Alireza ; Otranto, Domenico</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-20bf34358c435960da5c9c176f851c2adbbaee0fcee19fd64ad601ba290645363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Anaplasma</topic><topic>Babesia</topic><topic>Bartonella</topic><topic>Cats</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Dogs</topic><topic>Genetic testing</topic><topic>Humidity</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Mycoplasma</topic><topic>one health</topic><topic>Parasites</topic><topic>Pathogens</topic><topic>Public awareness</topic><topic>Public health</topic><topic>Real time</topic><topic>Saudi Arabia</topic><topic>Vector-borne diseases</topic><topic>Vectors (Biology)</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alanazi, Abdullah D</creatorcontrib><creatorcontrib>Alouffi, Abdulaziz S</creatorcontrib><creatorcontrib>Alyousif, Mohamed S</creatorcontrib><creatorcontrib>Alshahrani, Mohammad Y</creatorcontrib><creatorcontrib>Abdullah, Hend H A M</creatorcontrib><creatorcontrib>Abdel-Shafy, Sobhy</creatorcontrib><creatorcontrib>Calvani, Nichola Eliza Davies</creatorcontrib><creatorcontrib>Ansari-Lari, Maryam</creatorcontrib><creatorcontrib>Sazmand, Alireza</creatorcontrib><creatorcontrib>Otranto, Domenico</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Pathogens (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alanazi, Abdullah D</au><au>Alouffi, Abdulaziz S</au><au>Alyousif, Mohamed S</au><au>Alshahrani, Mohammad Y</au><au>Abdullah, Hend H A M</au><au>Abdel-Shafy, Sobhy</au><au>Calvani, Nichola Eliza Davies</au><au>Ansari-Lari, Maryam</au><au>Sazmand, Alireza</au><au>Otranto, Domenico</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Survey of Vector-Borne Pathogens of Dogs and Cats in Two Regions of Saudi Arabia</atitle><jtitle>Pathogens (Basel)</jtitle><addtitle>Pathogens</addtitle><date>2020-12-31</date><risdate>2020</risdate><volume>10</volume><issue>1</issue><spage>25</spage><pages>25-</pages><issn>2076-0817</issn><eissn>2076-0817</eissn><abstract>Dogs and cats play an important role as reservoirs of vector-borne pathogens, yet reports of canine and feline vector-borne diseases in Saudi Arabia are scarce. Blood samples were collected from 188 free-roaming dogs and cats in Asir (70 dogs and 44 cats) and Riyadh (74 dogs), Saudi Arabia. The presence of
spp.,
spp., hemotropic
spp.,
spp., and
spp. was detected using a multiplex tandem real-time PCR. PCR-positive samples were further examined with specific conventional and real-time PCR followed by sequencing. Dogs from Riyadh tested negative for all pathogens, while 46 out of 70 dogs (65.7%) and 17 out of 44 cats (38.6%) from Asir were positive for at least one pathogen. Positive dogs were infected with
(57.1%),
(30%),
(15.7%), and
(1.4%), and cats were infected with
(13.6%),
Mycoplasma haemominutum (13.6%),
(9.2%), and
(2.27%), all of which are reported for the first time in Saudi Arabia. Co-infection with
and
was detected in 17 dogs (24.28%), while coinfections were not detected in cats. These results suggest that effective control and public awareness strategies for minimizing infection in animals are necessary.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33396491</pmid><doi>10.3390/pathogens10010025</doi><orcidid>https://orcid.org/0000-0002-4862-7668</orcidid><orcidid>https://orcid.org/0000-0002-8450-2993</orcidid><orcidid>https://orcid.org/0000-0002-7518-476X</orcidid><orcidid>https://orcid.org/0000-0001-6462-585X</orcidid><orcidid>https://orcid.org/0000-0001-8863-3870</orcidid><orcidid>https://orcid.org/0000-0002-7096-0221</orcidid><orcidid>https://orcid.org/0000-0002-8787-6755</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 2076-0817 |
ispartof | Pathogens (Basel), 2020-12, Vol.10 (1), p.25 |
issn | 2076-0817 2076-0817 |
language | eng |
recordid | cdi_doaj_primary_oai_doaj_org_article_39e7a57212c14034901a57cddad99dfa |
source | Publicly Available Content Database; PubMed Central |
subjects | Anaplasma Babesia Bartonella Cats Deoxyribonucleic acid DNA Dogs Genetic testing Humidity Infections Infectious diseases Mycoplasma one health Parasites Pathogens Public awareness Public health Real time Saudi Arabia Vector-borne diseases Vectors (Biology) Zoonoses |
title | Molecular Survey of Vector-Borne Pathogens of Dogs and Cats in Two Regions of Saudi Arabia |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T23%3A19%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20Survey%20of%20Vector-Borne%20Pathogens%20of%20Dogs%20and%20Cats%20in%20Two%20Regions%20of%20Saudi%20Arabia&rft.jtitle=Pathogens%20(Basel)&rft.au=Alanazi,%20Abdullah%20D&rft.date=2020-12-31&rft.volume=10&rft.issue=1&rft.spage=25&rft.pages=25-&rft.issn=2076-0817&rft.eissn=2076-0817&rft_id=info:doi/10.3390/pathogens10010025&rft_dat=%3Cproquest_doaj_%3E2475019625%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c493t-20bf34358c435960da5c9c176f851c2adbbaee0fcee19fd64ad601ba290645363%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2475019625&rft_id=info:pmid/33396491&rfr_iscdi=true |