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Molecular Survey of Vector-Borne Pathogens of Dogs and Cats in Two Regions of Saudi Arabia

Dogs and cats play an important role as reservoirs of vector-borne pathogens, yet reports of canine and feline vector-borne diseases in Saudi Arabia are scarce. Blood samples were collected from 188 free-roaming dogs and cats in Asir (70 dogs and 44 cats) and Riyadh (74 dogs), Saudi Arabia. The pres...

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Published in:Pathogens (Basel) 2020-12, Vol.10 (1), p.25
Main Authors: Alanazi, Abdullah D, Alouffi, Abdulaziz S, Alyousif, Mohamed S, Alshahrani, Mohammad Y, Abdullah, Hend H A M, Abdel-Shafy, Sobhy, Calvani, Nichola Eliza Davies, Ansari-Lari, Maryam, Sazmand, Alireza, Otranto, Domenico
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creator Alanazi, Abdullah D
Alouffi, Abdulaziz S
Alyousif, Mohamed S
Alshahrani, Mohammad Y
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Calvani, Nichola Eliza Davies
Ansari-Lari, Maryam
Sazmand, Alireza
Otranto, Domenico
description Dogs and cats play an important role as reservoirs of vector-borne pathogens, yet reports of canine and feline vector-borne diseases in Saudi Arabia are scarce. Blood samples were collected from 188 free-roaming dogs and cats in Asir (70 dogs and 44 cats) and Riyadh (74 dogs), Saudi Arabia. The presence of spp., spp., hemotropic spp., spp., and spp. was detected using a multiplex tandem real-time PCR. PCR-positive samples were further examined with specific conventional and real-time PCR followed by sequencing. Dogs from Riyadh tested negative for all pathogens, while 46 out of 70 dogs (65.7%) and 17 out of 44 cats (38.6%) from Asir were positive for at least one pathogen. Positive dogs were infected with (57.1%), (30%), (15.7%), and (1.4%), and cats were infected with (13.6%), Mycoplasma haemominutum (13.6%), (9.2%), and (2.27%), all of which are reported for the first time in Saudi Arabia. Co-infection with and was detected in 17 dogs (24.28%), while coinfections were not detected in cats. These results suggest that effective control and public awareness strategies for minimizing infection in animals are necessary.
doi_str_mv 10.3390/pathogens10010025
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subjects Anaplasma
Babesia
Bartonella
Cats
Deoxyribonucleic acid
DNA
Dogs
Genetic testing
Humidity
Infections
Infectious diseases
Mycoplasma
one health
Parasites
Pathogens
Public awareness
Public health
Real time
Saudi Arabia
Vector-borne diseases
Vectors (Biology)
Zoonoses
title Molecular Survey of Vector-Borne Pathogens of Dogs and Cats in Two Regions of Saudi Arabia
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