Mulberry Leaf ( Morus alba L.) Extracts and Its Chlorogenic Acid Isomer Component Improve Glucolipotoxicity-Induced Hepatic Lipid Accumulation via Downregulating miR-34a and Decreased Inflammation

• Published in: Nutrients 2022-11, Vol.14 (22), p.4808
• Main Authors: Yang, Tsung-Yuan, Yu, Meng-Hsun, Wu, Yi-Liang, Hong, Ching-Chun, Chen, Chin-Shuh, Chan, Kuei-Chuan, Wang, Chau-Jong
• Format: Article
• Language: English
• Subjects: Accumulation; Care and treatment; Chemical properties; Chlorogenic acid; Chlorogenic Acid - metabolism; Chlorogenic Acid - pharmacology; Cholesterol; Complications and side effects; Development and progression; Diabetes; Diabetes mellitus; Enzymes; Fatty acids; Fatty liver; Fatty Liver - metabolism; Glucose; Health aspects; hepatic lipid accumulation and inflammation; Homeostasis; Hyperlipidemia; Hypertension; Inflammation; Inflammation - drug therapy; Inflammation - metabolism; Insulin resistance; Interleukin 6; Kinases; Leaves; Lipids; Liver; Materia medica, Vegetable; Metabolic disorders; Metabolism; MicroRNAs - genetics; MicroRNAs - metabolism; miR-34a; Morus; Morus alba; Mulberry; mulberry leaf extract; Natural & organic foods; neochlorogenic acid; NF-κB protein; nonalcoholic fatty liver disease; Oxidation; Pathogenesis; Physiological aspects; Plant extracts; Plant Extracts - metabolism; Plant Extracts - pharmacology; Plant Leaves - metabolism; Proteins; Risk factors; SIRT1 protein; Sterol Regulatory Element Binding Protein 1 - genetics; Sterol Regulatory Element Binding Protein 1 - metabolism; Sterol regulatory element-binding protein; Tumor necrosis factor-TNF; Tumor necrosis factor-α
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu14224808

Mulberry leaf ( L.) is used as a traditional medicine and potential health food to treat various metabolic diseases, such as hypertension, diabetes, and hyperlipidemia. However, we sought the mechanisms by which functional components of mulberry leaves mediate diabetic steatohepatitis. We applied an in vitro model of HepG2 cells induced by glucolipotoxicity and evaluated the effects of MLE and its major components nCGA, Crp, and CGA. The results showed that MLE and nCGA reduced liver fat accumulation by inhibiting SREBP-1/FASN, SREBP-2/HMG-CoAR, and activating PPARα/CPT-1. Additionally, MLE and nCGA decreased inflammatory responses associated with NF-κB, TNF-α, and IL-6 to alleviate steatohepatitis. Furthermore, we showed that MLE and nCGA exerted anti-glucolipotoxicity effects by downregulating miR-34a, thus activating SIRT1/AMPK signaling, and subsequently suppressing hepatic lipid accumulation.