Extraction, Characterization and Immunological Activity of Polysaccharides from Rhizoma gastrodiae

A response surface and Box-Behnken design approach was applied to augment polysaccharide extraction from the residue of Rhizoma gastrodiae. Statistical analysis revealed that the linear and quadratic terms for three variables during extraction exhibited obvious effects on extraction yield. The optim...

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Bibliographic Details
Published in:International journal of molecular sciences 2016-07, Vol.17 (7), p.1011-1011
Main Authors: Chen, Juncheng, Tian, Shan, Shu, Xiaoying, Du, Hongtao, Li, Na, Wang, Junru
Format: Article
Language:English
Subjects:
Animals
Carbohydrates
Cell Line
characterization
Extraction processes
Herbs
Immunologic Factors - chemistry
Immunologic Factors - pharmacology
immunological activity
Immunology
Macrophages - drug effects
Macrophages - immunology
Mice
Nitric Oxide - metabolism
optimized extraction
Orchidaceae - chemistry
Phagocytosis
Plant Extracts - chemistry
Plant Extracts - pharmacology
polysaccharides
Polysaccharides - chemistry
Polysaccharides - pharmacology
Rhizoma gastrodiae
Rhizome - chemistry
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Summary:A response surface and Box-Behnken design approach was applied to augment polysaccharide extraction from the residue of Rhizoma gastrodiae. Statistical analysis revealed that the linear and quadratic terms for three variables during extraction exhibited obvious effects on extraction yield. The optimum conditions were determined to be a liquid-to-solid ratio of 54 mL/g, an extraction temperature of 74 °C, an extraction time of 66 min, and three extractions. These conditions resulted in a maximum Rhizoma gastrodiae polysaccharide (RGP) extraction yield of 6.11% ± 0.13%. Two homogeneous polysaccharides (RGP-1a and RGP-1b) were obtained using DEAE cellulose-52 and Sephadex G-100 columns. The preliminary characterization of RGP-1a and RGP-1b was performed using HPLC-RID, HPGPC, and FTIR. Tests of the immunological activity in vitro showed that the two polysaccharides could significantly stimulate macrophages to release NO and enhance phagocytosis in a dose-dependent manner. In particular, RGP-1b (200 μg/mL) and LPS (2 μg/mL) had almost the same influence on the NO production and phagocytic activity of RAW 264.7 macrophages (p > 0.05). All the data obtained indicate that RGP-1a and RGP-1b have the potential to be developed as a health food.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms17071011