DNA barcoding ferns in an unexplored tropical montane cloud forest area of southeast Oaxaca, Mexico

DNA barcoding can be useful for species identification and phylogenetic analysis, but its effectivity has not been verified in most neotropical cloud forest plants. We tested three plastid barcodes, rbcLa, matK , and trnH-psbA , in selected pteridophytes, a well-represented group in these forests, f...

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Bibliographic Details
Published in:Scientific reports 2021-11, Vol.11 (1), p.22837-22837, Article 22837
Main Authors: Trujillo-Argueta, Sonia, del Castillo, Rafael F., Tejero-Diez, Daniel, Matias-Cervantes, Carlos Alberto, Velasco-Murguía, Abril
Format: Article
Language:English
Subjects:
631/1647
631/337
631/449
Bayesian analysis
Cloud forests
Deoxyribonucleic acid
Divergence
DNA
DNA Barcoding, Taxonomic
Ferns
Ferns - classification
Ferns - genetics
Ferns - growth & development
Forests
Gene Expression Regulation, Plant
Genes, Plant
Genetic analysis
Genetic distance
Humanities and Social Sciences
Mexico
multidisciplinary
Phylogenetics
Phylogeny
Science
Science (multidisciplinary)
Species
Species Specificity
Tropical Climate
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Summary:DNA barcoding can be useful for species identification and phylogenetic analysis, but its effectivity has not been verified in most neotropical cloud forest plants. We tested three plastid barcodes, rbcLa, matK , and trnH-psbA , in selected pteridophytes, a well-represented group in these forests, from a little-explored area in Oaxaca, Mexico, applying the CBOL criteria for barcoding. We used BLASTn, genetic distance, and monophyly tree-based analyses employing neighbor-joining (NJ), maximum likelihood (ML), and Bayesian inference methods. Universal primers for rbcLa and trnH-psbA were successfully amplified and bi-directionally sequenced, but matK could not be amplified for most species. rbcLa showed the highest species discrimination in BLASTn (66.67%). trnH-psbA exhibited higher significant interspecific divergence values than rbcL and rbcLa  +  trnH-psbA (two-sample sign test, P value 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-02237-8