PPARγ regulates the expression of genes involved in the DNA damage response in an inflamed endometrium

Inflammation is a biological response of the immune system, which can be triggered by many factors, including pathogens. These factors may induce acute or chronic inflammation in various organs, including the reproductive system, leading to tissue damage or disease. In this study, the RNA-Seq techni...

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Published in:Scientific reports 2022-03, Vol.12 (1), p.4026-4026, Article 4026
Main Authors: Mierzejewski, Karol, Paukszto, Łukasz, Kurzyńska, Aleksandra, Kunicka, Zuzanna, Jastrzębski, Jan P., Makowczenko, Karol G., Golubska, Monika, Bogacka, Iwona
Format: Article
Language:English
Subjects:
631/208/199
631/250/256
Animals
DNA Damage
Endometrium - metabolism
Female
Humanities and Social Sciences
Inflammation - metabolism
Ligands
Lipopolysaccharides - metabolism
multidisciplinary
Pioglitazone - adverse effects
PPAR gamma - metabolism
Prostaglandin D2 - metabolism
RNA, Long Noncoding - metabolism
Science
Science (multidisciplinary)
Swine
Thiazolidinediones - adverse effects
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Summary:Inflammation is a biological response of the immune system, which can be triggered by many factors, including pathogens. These factors may induce acute or chronic inflammation in various organs, including the reproductive system, leading to tissue damage or disease. In this study, the RNA-Seq technique was used to determine the in vitro effects of peroxisome proliferator-activated receptor gamma (PPARγ) ligands on the expression of genes and long non-coding RNA, and alternative splicing events (ASEs) in LPS-induced inflammation of the porcine endometrium during the follicular phase of the estrous cycle. Endometrial slices were incubated in the presence of LPS and PPARγ agonists (PGJ 2 or pioglitazone) and a PPARγ antagonist (T0070907). We identified 169, 200, 599 and 557 differentially expressed genes after LPS, PGJ 2 , pioglitazone or T0070907 treatment, respectively. Moreover, changes in differentially expressed long non-coding RNA and differential alternative splicing events were described after the treatments. The study revealed that PPARγ ligands influence the LPS-triggered expression of genes controlling the DNA damage response ( GADD45β, CDK1, CCNA1, CCNG1, ATM ). Pioglitazone treatment exerted a considerable effect on the expression of genes regulating the DNA damage response.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-07986-8