Mesenchymal stem cells alleviate airway inflammation via modulation of T-helper 17/regulatory T cells balance in mice with ovalbumin-induced asthma

• Published in: Chinese medical journal 2021-09, Vol.134 (17), p.2116-2118
• Main Authors: Zeng, Shao-Lin, Cui, Juan, Hu, Ke, Nie, Han-Xiang, Zhang, Yun-Ting
• Format: Article
• Language: English
• Subjects: Airway management; Animals; Asthma; Asthma - immunology; Asthma - therapy; Bone marrow; Bronchoalveolar Lavage Fluid; Connective tissue; Cytokines; Disease Models, Animal; Inflammation; Inflammation - therapy; Laboratory animals; Lung; Lymphocytes; Mesenchymal Stem Cells; Mice; Mice, Inbred BALB C; Neutrophils; Ostomy; Ovalbumin; Rapid Report; Stem cells; T-Lymphocytes, Regulatory - immunology; Th17 Cells - immunology
• ISSN: 0366-6999; 2542-5641
• DOI: 10.1097/CM9.0000000000001699

Female BALB/c mice (n = 8 for each group; 16–24 g, 6–8 weeks) were provided by the Laboratory Animal Center of Wuhan University (Wuhan, China). [1] BALB/c mice were divided into three groups: (1) control group: mice were sensitized with phosphate buffer saline (PBS), challenged with OVA and treated with PBS; (2) asthma model group (OVA group): mice were sensitized and challenged with OVA and treated with PBS; (3) MSCs treatment group (OVA + MSCs group): mice were sensitized and challenged with OVA and treated with 106 MSCs which were injected via tail vein. Flow cytometric analysis of splenocytes revealed markedly elevated population of CD4+IL-17A+Th17 cells in OVA group compared with the normal control group ([26.14 ± 4.21]% vs. Varieties of medical remedies for asthma relief have diverse side effects and limited efficacies after long-time treatment. [...]new effective therapeutic approach is desirable.