Loading…
Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection
Salmonella Typhimurium causes a self-limiting gastroenteritis that may lead to systemic disease. Bacteria invade the small intestine, crossing the intestinal epithelium from where they are transported to the mesenteric lymph nodes (MLNs) within migrating immune cells. MLNs are an important site at w...
Saved in:
Published in: | Scientific reports 2017-06, Vol.7 (1), p.2786-13, Article 2786 |
---|---|
Main Authors: | , , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c577t-b13936fe80c969c548f3d758b512c840a8711606a5d584dd0932e05086cd29ed3 |
---|---|
cites | cdi_FETCH-LOGICAL-c577t-b13936fe80c969c548f3d758b512c840a8711606a5d584dd0932e05086cd29ed3 |
container_end_page | 13 |
container_issue | 1 |
container_start_page | 2786 |
container_title | Scientific reports |
container_volume | 7 |
creator | Hulme, Heather E. Meikle, Lynsey M. Wessel, Hannah Strittmatter, Nicole Swales, John Thomson, Carolyn Nilsson, Anna Nibbs, Robert J. B. Milling, Simon Andren, Per E. Mackay, C. Logan Dexter, Alex Bunch, Josephine Goodwin, Richard J. A. Burchmore, Richard Wall, Daniel M. |
description | Salmonella
Typhimurium causes a self-limiting gastroenteritis that may lead to systemic disease. Bacteria invade the small intestine, crossing the intestinal epithelium from where they are transported to the mesenteric lymph nodes (MLNs) within migrating immune cells. MLNs are an important site at which the innate and adaptive immune responses converge but their architecture and function is severely disrupted during
S
. Typhimurium infection. To further understand host-pathogen interactions at this site, we used mass spectrometry imaging (MSI) to analyse MLN tissue from a murine model of
S
. Typhimurium infection. A molecule, identified as palmitoylcarnitine (PalC), was of particular interest due to its high abundance at loci of
S
. Typhimurium infection and MLN disruption. High levels of PalC localised to sites within the MLNs where B and T cells were absent and where the perimeter of CD169
+
sub capsular sinus macrophages was disrupted. MLN cells cultured
ex vivo
and treated with PalC had reduced CD4
+
CD25
+
T cells and an increased number of B220
+
CD19
+
B cells. The reduction in CD4
+
CD25
+
T cells was likely due to apoptosis driven by increased caspase-3/7 activity. These data indicate that PalC significantly alters the host response in the MLNs, acting as a decisive factor in infection outcome. |
doi_str_mv | 10.1038/s41598-017-03100-5 |
format | article |
fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_3a24c8a8c4bd40bd88a9c66a0de21e92</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_3a24c8a8c4bd40bd88a9c66a0de21e92</doaj_id><sourcerecordid>1906465706</sourcerecordid><originalsourceid>FETCH-LOGICAL-c577t-b13936fe80c969c548f3d758b512c840a8711606a5d584dd0932e05086cd29ed3</originalsourceid><addsrcrecordid>eNp1ks1vFCEYxidGY5vaf8CDIfHiwVG-By4mTf1qUuPB6pUwwGzZzMAIMzWb9I8vu7M2uyZygcDvfeB9eKrqJYLvECTifaaISVFD1NSQIAhr9qQ6xZCyGhOMnx6sT6rznNewDIYlRfJ5dYIFExQLdFrdf9M5gzw6M6U4uCltgB_0yocV8NaFyXfeZTDqfvBT3PRGp-AnHxzQGehQ2GEOsY8rb3QPBme9nmICdk5bhR-lLAbX9xrcbMZbP5TteQA-dOU6H8OL6lmn--zO9_NZ9fPzp5vLr_X19y9XlxfXtWFNM9UtIpLwzgloJJeGUdER2zDRMoSNoFCLBiEOuWa2tGUtlAQ7yKDgxmLpLDmrrhZdG_Vajal0mDYqaq92GzGtlE6TN71TRGNqhBaGtpbC1gqhpeFcQ-swchIXrbeLVv7jxrk9Uvvof13s1OZZEdxgTAv-YcELW-wxxdKk-6Oq45Pgb9Uq3ilGmWykLAJv9gIp_p5dntTgs9l6Glycs0IScspZA3lBX_-DruOcQnG2UIwxzBuKCoUXyqSYc3Ld42MQVNtoqSVaqkRL7aKlWCl6ddjGY8nfIBWA7G0Zt1_v0sHd_5d9AOvf3JM</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1955526741</pqid></control><display><type>article</type><title>Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection</title><source>PubMed Central (Open Access)</source><source>ProQuest - Publicly Available Content Database</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature - nature.com Journals - Fully Open Access</source><creator>Hulme, Heather E. ; Meikle, Lynsey M. ; Wessel, Hannah ; Strittmatter, Nicole ; Swales, John ; Thomson, Carolyn ; Nilsson, Anna ; Nibbs, Robert J. B. ; Milling, Simon ; Andren, Per E. ; Mackay, C. Logan ; Dexter, Alex ; Bunch, Josephine ; Goodwin, Richard J. A. ; Burchmore, Richard ; Wall, Daniel M.</creator><creatorcontrib>Hulme, Heather E. ; Meikle, Lynsey M. ; Wessel, Hannah ; Strittmatter, Nicole ; Swales, John ; Thomson, Carolyn ; Nilsson, Anna ; Nibbs, Robert J. B. ; Milling, Simon ; Andren, Per E. ; Mackay, C. Logan ; Dexter, Alex ; Bunch, Josephine ; Goodwin, Richard J. A. ; Burchmore, Richard ; Wall, Daniel M.</creatorcontrib><description>Salmonella
Typhimurium causes a self-limiting gastroenteritis that may lead to systemic disease. Bacteria invade the small intestine, crossing the intestinal epithelium from where they are transported to the mesenteric lymph nodes (MLNs) within migrating immune cells. MLNs are an important site at which the innate and adaptive immune responses converge but their architecture and function is severely disrupted during
S
. Typhimurium infection. To further understand host-pathogen interactions at this site, we used mass spectrometry imaging (MSI) to analyse MLN tissue from a murine model of
S
. Typhimurium infection. A molecule, identified as palmitoylcarnitine (PalC), was of particular interest due to its high abundance at loci of
S
. Typhimurium infection and MLN disruption. High levels of PalC localised to sites within the MLNs where B and T cells were absent and where the perimeter of CD169
+
sub capsular sinus macrophages was disrupted. MLN cells cultured
ex vivo
and treated with PalC had reduced CD4
+
CD25
+
T cells and an increased number of B220
+
CD19
+
B cells. The reduction in CD4
+
CD25
+
T cells was likely due to apoptosis driven by increased caspase-3/7 activity. These data indicate that PalC significantly alters the host response in the MLNs, acting as a decisive factor in infection outcome.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-017-03100-5</identifier><identifier>PMID: 28584281</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13 ; 13/31 ; 14 ; 631/1647/245 ; 631/250/254 ; 631/326/421 ; 64 ; 64/60 ; Adaptive immunity ; Animal models ; Animals ; Apoptosis ; Biomarkers ; Caspase ; Caspase-3 ; CD19 antigen ; CD25 antigen ; CD4 antigen ; Epithelium ; Female ; Gastroenteritis ; Host-pathogen interactions ; Humanities and Social Sciences ; Immune response ; Immunologic Factors - metabolism ; Infections ; Lymph nodes ; Lymphocytes ; Lymphocytes B ; Lymphocytes T ; Macrophages ; Mass Spectrometry ; Mass spectroscopy ; Mice ; multidisciplinary ; Palmitoylcarnitine - metabolism ; Salmonella ; Salmonella Infections, Animal - immunology ; Salmonella Infections, Animal - metabolism ; Salmonella Infections, Animal - microbiology ; Salmonella Infections, Animal - pathology ; Salmonella typhimurium - immunology ; Science ; Science (multidisciplinary) ; Scientific imaging ; Sinus ; Small intestine ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><ispartof>Scientific reports, 2017-06, Vol.7 (1), p.2786-13, Article 2786</ispartof><rights>The Author(s) 2017</rights><rights>Copyright Nature Publishing Group Jun 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c577t-b13936fe80c969c548f3d758b512c840a8711606a5d584dd0932e05086cd29ed3</citedby><cites>FETCH-LOGICAL-c577t-b13936fe80c969c548f3d758b512c840a8711606a5d584dd0932e05086cd29ed3</cites><orcidid>0000-0003-4384-5516</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1955526741/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1955526741?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28584281$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-327224$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Hulme, Heather E.</creatorcontrib><creatorcontrib>Meikle, Lynsey M.</creatorcontrib><creatorcontrib>Wessel, Hannah</creatorcontrib><creatorcontrib>Strittmatter, Nicole</creatorcontrib><creatorcontrib>Swales, John</creatorcontrib><creatorcontrib>Thomson, Carolyn</creatorcontrib><creatorcontrib>Nilsson, Anna</creatorcontrib><creatorcontrib>Nibbs, Robert J. B.</creatorcontrib><creatorcontrib>Milling, Simon</creatorcontrib><creatorcontrib>Andren, Per E.</creatorcontrib><creatorcontrib>Mackay, C. Logan</creatorcontrib><creatorcontrib>Dexter, Alex</creatorcontrib><creatorcontrib>Bunch, Josephine</creatorcontrib><creatorcontrib>Goodwin, Richard J. A.</creatorcontrib><creatorcontrib>Burchmore, Richard</creatorcontrib><creatorcontrib>Wall, Daniel M.</creatorcontrib><title>Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Salmonella
Typhimurium causes a self-limiting gastroenteritis that may lead to systemic disease. Bacteria invade the small intestine, crossing the intestinal epithelium from where they are transported to the mesenteric lymph nodes (MLNs) within migrating immune cells. MLNs are an important site at which the innate and adaptive immune responses converge but their architecture and function is severely disrupted during
S
. Typhimurium infection. To further understand host-pathogen interactions at this site, we used mass spectrometry imaging (MSI) to analyse MLN tissue from a murine model of
S
. Typhimurium infection. A molecule, identified as palmitoylcarnitine (PalC), was of particular interest due to its high abundance at loci of
S
. Typhimurium infection and MLN disruption. High levels of PalC localised to sites within the MLNs where B and T cells were absent and where the perimeter of CD169
+
sub capsular sinus macrophages was disrupted. MLN cells cultured
ex vivo
and treated with PalC had reduced CD4
+
CD25
+
T cells and an increased number of B220
+
CD19
+
B cells. The reduction in CD4
+
CD25
+
T cells was likely due to apoptosis driven by increased caspase-3/7 activity. These data indicate that PalC significantly alters the host response in the MLNs, acting as a decisive factor in infection outcome.</description><subject>13</subject><subject>13/31</subject><subject>14</subject><subject>631/1647/245</subject><subject>631/250/254</subject><subject>631/326/421</subject><subject>64</subject><subject>64/60</subject><subject>Adaptive immunity</subject><subject>Animal models</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Biomarkers</subject><subject>Caspase</subject><subject>Caspase-3</subject><subject>CD19 antigen</subject><subject>CD25 antigen</subject><subject>CD4 antigen</subject><subject>Epithelium</subject><subject>Female</subject><subject>Gastroenteritis</subject><subject>Host-pathogen interactions</subject><subject>Humanities and Social Sciences</subject><subject>Immune response</subject><subject>Immunologic Factors - metabolism</subject><subject>Infections</subject><subject>Lymph nodes</subject><subject>Lymphocytes</subject><subject>Lymphocytes B</subject><subject>Lymphocytes T</subject><subject>Macrophages</subject><subject>Mass Spectrometry</subject><subject>Mass spectroscopy</subject><subject>Mice</subject><subject>multidisciplinary</subject><subject>Palmitoylcarnitine - metabolism</subject><subject>Salmonella</subject><subject>Salmonella Infections, Animal - immunology</subject><subject>Salmonella Infections, Animal - metabolism</subject><subject>Salmonella Infections, Animal - microbiology</subject><subject>Salmonella Infections, Animal - pathology</subject><subject>Salmonella typhimurium - immunology</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Scientific imaging</subject><subject>Sinus</subject><subject>Small intestine</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp1ks1vFCEYxidGY5vaf8CDIfHiwVG-By4mTf1qUuPB6pUwwGzZzMAIMzWb9I8vu7M2uyZygcDvfeB9eKrqJYLvECTifaaISVFD1NSQIAhr9qQ6xZCyGhOMnx6sT6rznNewDIYlRfJ5dYIFExQLdFrdf9M5gzw6M6U4uCltgB_0yocV8NaFyXfeZTDqfvBT3PRGp-AnHxzQGehQ2GEOsY8rb3QPBme9nmICdk5bhR-lLAbX9xrcbMZbP5TteQA-dOU6H8OL6lmn--zO9_NZ9fPzp5vLr_X19y9XlxfXtWFNM9UtIpLwzgloJJeGUdER2zDRMoSNoFCLBiEOuWa2tGUtlAQ7yKDgxmLpLDmrrhZdG_Vajal0mDYqaq92GzGtlE6TN71TRGNqhBaGtpbC1gqhpeFcQ-swchIXrbeLVv7jxrk9Uvvof13s1OZZEdxgTAv-YcELW-wxxdKk-6Oq45Pgb9Uq3ilGmWykLAJv9gIp_p5dntTgs9l6Glycs0IScspZA3lBX_-DruOcQnG2UIwxzBuKCoUXyqSYc3Ld42MQVNtoqSVaqkRL7aKlWCl6ddjGY8nfIBWA7G0Zt1_v0sHd_5d9AOvf3JM</recordid><startdate>20170605</startdate><enddate>20170605</enddate><creator>Hulme, Heather E.</creator><creator>Meikle, Lynsey M.</creator><creator>Wessel, Hannah</creator><creator>Strittmatter, Nicole</creator><creator>Swales, John</creator><creator>Thomson, Carolyn</creator><creator>Nilsson, Anna</creator><creator>Nibbs, Robert J. B.</creator><creator>Milling, Simon</creator><creator>Andren, Per E.</creator><creator>Mackay, C. Logan</creator><creator>Dexter, Alex</creator><creator>Bunch, Josephine</creator><creator>Goodwin, Richard J. A.</creator><creator>Burchmore, Richard</creator><creator>Wall, Daniel M.</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><general>Nature Portfolio</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>ACNBI</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>DF2</scope><scope>ZZAVC</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-4384-5516</orcidid></search><sort><creationdate>20170605</creationdate><title>Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection</title><author>Hulme, Heather E. ; Meikle, Lynsey M. ; Wessel, Hannah ; Strittmatter, Nicole ; Swales, John ; Thomson, Carolyn ; Nilsson, Anna ; Nibbs, Robert J. B. ; Milling, Simon ; Andren, Per E. ; Mackay, C. Logan ; Dexter, Alex ; Bunch, Josephine ; Goodwin, Richard J. A. ; Burchmore, Richard ; Wall, Daniel M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c577t-b13936fe80c969c548f3d758b512c840a8711606a5d584dd0932e05086cd29ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>13</topic><topic>13/31</topic><topic>14</topic><topic>631/1647/245</topic><topic>631/250/254</topic><topic>631/326/421</topic><topic>64</topic><topic>64/60</topic><topic>Adaptive immunity</topic><topic>Animal models</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Biomarkers</topic><topic>Caspase</topic><topic>Caspase-3</topic><topic>CD19 antigen</topic><topic>CD25 antigen</topic><topic>CD4 antigen</topic><topic>Epithelium</topic><topic>Female</topic><topic>Gastroenteritis</topic><topic>Host-pathogen interactions</topic><topic>Humanities and Social Sciences</topic><topic>Immune response</topic><topic>Immunologic Factors - metabolism</topic><topic>Infections</topic><topic>Lymph nodes</topic><topic>Lymphocytes</topic><topic>Lymphocytes B</topic><topic>Lymphocytes T</topic><topic>Macrophages</topic><topic>Mass Spectrometry</topic><topic>Mass spectroscopy</topic><topic>Mice</topic><topic>multidisciplinary</topic><topic>Palmitoylcarnitine - metabolism</topic><topic>Salmonella</topic><topic>Salmonella Infections, Animal - immunology</topic><topic>Salmonella Infections, Animal - metabolism</topic><topic>Salmonella Infections, Animal - microbiology</topic><topic>Salmonella Infections, Animal - pathology</topic><topic>Salmonella typhimurium - immunology</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Scientific imaging</topic><topic>Sinus</topic><topic>Small intestine</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hulme, Heather E.</creatorcontrib><creatorcontrib>Meikle, Lynsey M.</creatorcontrib><creatorcontrib>Wessel, Hannah</creatorcontrib><creatorcontrib>Strittmatter, Nicole</creatorcontrib><creatorcontrib>Swales, John</creatorcontrib><creatorcontrib>Thomson, Carolyn</creatorcontrib><creatorcontrib>Nilsson, Anna</creatorcontrib><creatorcontrib>Nibbs, Robert J. B.</creatorcontrib><creatorcontrib>Milling, Simon</creatorcontrib><creatorcontrib>Andren, Per E.</creatorcontrib><creatorcontrib>Mackay, C. Logan</creatorcontrib><creatorcontrib>Dexter, Alex</creatorcontrib><creatorcontrib>Bunch, Josephine</creatorcontrib><creatorcontrib>Goodwin, Richard J. A.</creatorcontrib><creatorcontrib>Burchmore, Richard</creatorcontrib><creatorcontrib>Wall, Daniel M.</creatorcontrib><collection>Springer_OA刊</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Science Journals</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest - Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SWEPUB Uppsala universitet full text</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SWEPUB Uppsala universitet</collection><collection>SwePub Articles full text</collection><collection>Directory of Open Access Journals(OpenAccess)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hulme, Heather E.</au><au>Meikle, Lynsey M.</au><au>Wessel, Hannah</au><au>Strittmatter, Nicole</au><au>Swales, John</au><au>Thomson, Carolyn</au><au>Nilsson, Anna</au><au>Nibbs, Robert J. B.</au><au>Milling, Simon</au><au>Andren, Per E.</au><au>Mackay, C. Logan</au><au>Dexter, Alex</au><au>Bunch, Josephine</au><au>Goodwin, Richard J. A.</au><au>Burchmore, Richard</au><au>Wall, Daniel M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2017-06-05</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>2786</spage><epage>13</epage><pages>2786-13</pages><artnum>2786</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Salmonella
Typhimurium causes a self-limiting gastroenteritis that may lead to systemic disease. Bacteria invade the small intestine, crossing the intestinal epithelium from where they are transported to the mesenteric lymph nodes (MLNs) within migrating immune cells. MLNs are an important site at which the innate and adaptive immune responses converge but their architecture and function is severely disrupted during
S
. Typhimurium infection. To further understand host-pathogen interactions at this site, we used mass spectrometry imaging (MSI) to analyse MLN tissue from a murine model of
S
. Typhimurium infection. A molecule, identified as palmitoylcarnitine (PalC), was of particular interest due to its high abundance at loci of
S
. Typhimurium infection and MLN disruption. High levels of PalC localised to sites within the MLNs where B and T cells were absent and where the perimeter of CD169
+
sub capsular sinus macrophages was disrupted. MLN cells cultured
ex vivo
and treated with PalC had reduced CD4
+
CD25
+
T cells and an increased number of B220
+
CD19
+
B cells. The reduction in CD4
+
CD25
+
T cells was likely due to apoptosis driven by increased caspase-3/7 activity. These data indicate that PalC significantly alters the host response in the MLNs, acting as a decisive factor in infection outcome.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28584281</pmid><doi>10.1038/s41598-017-03100-5</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-4384-5516</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 2045-2322 |
ispartof | Scientific reports, 2017-06, Vol.7 (1), p.2786-13, Article 2786 |
issn | 2045-2322 2045-2322 |
language | eng |
recordid | cdi_doaj_primary_oai_doaj_org_article_3a24c8a8c4bd40bd88a9c66a0de21e92 |
source | PubMed Central (Open Access); ProQuest - Publicly Available Content Database; Free Full-Text Journals in Chemistry; Springer Nature - nature.com Journals - Fully Open Access |
subjects | 13 13/31 14 631/1647/245 631/250/254 631/326/421 64 64/60 Adaptive immunity Animal models Animals Apoptosis Biomarkers Caspase Caspase-3 CD19 antigen CD25 antigen CD4 antigen Epithelium Female Gastroenteritis Host-pathogen interactions Humanities and Social Sciences Immune response Immunologic Factors - metabolism Infections Lymph nodes Lymphocytes Lymphocytes B Lymphocytes T Macrophages Mass Spectrometry Mass spectroscopy Mice multidisciplinary Palmitoylcarnitine - metabolism Salmonella Salmonella Infections, Animal - immunology Salmonella Infections, Animal - metabolism Salmonella Infections, Animal - microbiology Salmonella Infections, Animal - pathology Salmonella typhimurium - immunology Science Science (multidisciplinary) Scientific imaging Sinus Small intestine Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
title | Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T08%3A08%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mass%20spectrometry%20imaging%20identifies%20palmitoylcarnitine%20as%20an%20immunological%20mediator%20during%20Salmonella%20Typhimurium%20infection&rft.jtitle=Scientific%20reports&rft.au=Hulme,%20Heather%20E.&rft.date=2017-06-05&rft.volume=7&rft.issue=1&rft.spage=2786&rft.epage=13&rft.pages=2786-13&rft.artnum=2786&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-017-03100-5&rft_dat=%3Cproquest_doaj_%3E1906465706%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c577t-b13936fe80c969c548f3d758b512c840a8711606a5d584dd0932e05086cd29ed3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1955526741&rft_id=info:pmid/28584281&rfr_iscdi=true |