Inactivation of Acanthamoeba Cysts in Suspension and on Contaminated Contact Lenses Using Non-Thermal Plasma

Water suspensions of cysts of a pathogenic clinical isolate of Acanthamoeba sp. were prepared, and the cysts were inactivated either in suspension or placed on the surface of contact lenses by the non-thermal plasma produced by the DC corona transient spark discharge. The efficacy of this treatment...

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Bibliographic Details
Published in:Microorganisms (Basel) 2021-09, Vol.9 (9), p.1879
Main Authors: Měřínská, Tereza, Scholtz, Vladimír, Khun, Josef, Julák, Jaroslav, Nohýnková, Eva
Format: Article
Language:English
Subjects:
Acanthamoeba
Bacteria
Contact lenses
Cornea
Cysts
DC corona discharge
Deactivation
Disinfection & disinfectants
E coli
Electric sparks
Electrodes
Experiments
Gases
Hydrogels
Inactivation
Infections
Infrared spectra
IR spectra
Microorganisms
Moisture content
Plasma
Raman spectra
Raman spectroscopy
Thermal plasmas
Trophozoites
Water content
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Summary:Water suspensions of cysts of a pathogenic clinical isolate of Acanthamoeba sp. were prepared, and the cysts were inactivated either in suspension or placed on the surface of contact lenses by the non-thermal plasma produced by the DC corona transient spark discharge. The efficacy of this treatment was determined by cultivation and the presence of vegetative trophozoites indicating non-inactivated cysts. The negative discharge appeared to be more effective than the positive one. The complete inactivation occurred in water suspension after 40 min and on contaminated lenses after 50 min of plasma exposure. The properties of lenses seem to not be affected by plasma exposure; that is, their optical power, diameter, curvature, water content and infrared and Raman spectra remain unchanged.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms9091879