Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine

The measurement of cysteine in human urine and live cells is crucial for evaluating biological metabolism, monitoring and maintaining the immune system, preventing tissue/DNA damage caused by free radicals, preventing autoimmune diseases, and diagnosing disorders such as cystinuria and cancer. A met...

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Bibliographic Details
Published in:Biosensors (Basel) 2021-10, Vol.11 (11), p.420
Main Authors: Yeom, Gyu Seong, Song, In-Ho, Warkad, Shrikant Dashrath, Shinde, Pramod B, Kim, Taewoon, Park, Seong-Min, Nimse, Satish Balasaheb
Format: Article
Language:English
Subjects:
Amino acids
Autoimmune diseases
Benzimidazoles
bio-imaging
Biomonitoring
biothiols
Chromatography
Cysteine
Cysteine - urine
Cystinuria
Damage prevention
DNA damage
Ethanol
Fluorescence
Fluorescent Dyes
Fluorescent indicators
fluorimeter
Fluorometers
Free radicals
Glutathione
Homocysteine
Human wastes
Humans
Immune system
portable
Portable equipment
Response time
Selectivity
Spectrometry, Fluorescence
Spectrum analysis
Urine
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Summary:The measurement of cysteine in human urine and live cells is crucial for evaluating biological metabolism, monitoring and maintaining the immune system, preventing tissue/DNA damage caused by free radicals, preventing autoimmune diseases, and diagnosing disorders such as cystinuria and cancer. A method that uses a fluorescence turn-on probe and a portable fluorescence spectrometer device are crucial for highly sensitive, simple, rapid, and inexpensive cysteine detection. Herein, we present the synthesis and application of a benzimidazole-based fluorescent probe ( ) along with the design and development of a portable fluorescence spectrometer device ( ) for detecting cysteine in simulated human urine. showed excellent selectivity and sensitivity in detecting cysteine over homocysteine, glutathione, and other amino acids with the response time of 1 min and demonstrated a detection limit of 16.3 nM using the developed . Further, also demonstrated its utility in detecting intracellular cysteine, making it an excellent probe for bio-imaging assay.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios11110420