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Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine
The measurement of cysteine in human urine and live cells is crucial for evaluating biological metabolism, monitoring and maintaining the immune system, preventing tissue/DNA damage caused by free radicals, preventing autoimmune diseases, and diagnosing disorders such as cystinuria and cancer. A met...
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| Published in: | Biosensors (Basel) 2021-10, Vol.11 (11), p.420 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c478t-c7c69e60d6578a2ac853f52b838c199f8bc4001f68b0d10ac69efa5eb3b7683 |
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| cites | cdi_FETCH-LOGICAL-c478t-c7c69e60d6578a2ac853f52b838c199f8bc4001f68b0d10ac69efa5eb3b7683 |
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| container_issue | 11 |
| container_start_page | 420 |
| container_title | Biosensors (Basel) |
| container_volume | 11 |
| creator | Yeom, Gyu Seong Song, In-Ho Warkad, Shrikant Dashrath Shinde, Pramod B Kim, Taewoon Park, Seong-Min Nimse, Satish Balasaheb |
| description | The measurement of cysteine in human urine and live cells is crucial for evaluating biological metabolism, monitoring and maintaining the immune system, preventing tissue/DNA damage caused by free radicals, preventing autoimmune diseases, and diagnosing disorders such as cystinuria and cancer. A method that uses a fluorescence turn-on probe and a portable fluorescence spectrometer device are crucial for highly sensitive, simple, rapid, and inexpensive cysteine detection. Herein, we present the synthesis and application of a benzimidazole-based fluorescent probe (
) along with the design and development of a portable fluorescence spectrometer device (
) for detecting cysteine in simulated human urine.
showed excellent selectivity and sensitivity in detecting cysteine over homocysteine, glutathione, and other amino acids with the response time of 1 min and demonstrated a detection limit of 16.3 nM using the developed
. Further,
also demonstrated its utility in detecting intracellular cysteine, making it an excellent probe for bio-imaging assay. |
| doi_str_mv | 10.3390/bios11110420 |
| format | article |
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) along with the design and development of a portable fluorescence spectrometer device (
) for detecting cysteine in simulated human urine.
showed excellent selectivity and sensitivity in detecting cysteine over homocysteine, glutathione, and other amino acids with the response time of 1 min and demonstrated a detection limit of 16.3 nM using the developed
. Further,
also demonstrated its utility in detecting intracellular cysteine, making it an excellent probe for bio-imaging assay.</description><identifier>ISSN: 2079-6374</identifier><identifier>EISSN: 2079-6374</identifier><identifier>DOI: 10.3390/bios11110420</identifier><identifier>PMID: 34821635</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Amino acids ; Autoimmune diseases ; Benzimidazoles ; bio-imaging ; Biomonitoring ; biothiols ; Chromatography ; Cysteine ; Cysteine - urine ; Cystinuria ; Damage prevention ; DNA damage ; Ethanol ; Fluorescence ; Fluorescent Dyes ; Fluorescent indicators ; fluorimeter ; Fluorometers ; Free radicals ; Glutathione ; Homocysteine ; Human wastes ; Humans ; Immune system ; portable ; Portable equipment ; Response time ; Selectivity ; Spectrometry, Fluorescence ; Spectrum analysis ; Urine</subject><ispartof>Biosensors (Basel), 2021-10, Vol.11 (11), p.420</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-c7c69e60d6578a2ac853f52b838c199f8bc4001f68b0d10ac69efa5eb3b7683</citedby><cites>FETCH-LOGICAL-c478t-c7c69e60d6578a2ac853f52b838c199f8bc4001f68b0d10ac69efa5eb3b7683</cites><orcidid>0000-0002-5137-0584 ; 0000-0002-3173-0896</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2602001244/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2602001244?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,74998</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34821635$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yeom, Gyu Seong</creatorcontrib><creatorcontrib>Song, In-Ho</creatorcontrib><creatorcontrib>Warkad, Shrikant Dashrath</creatorcontrib><creatorcontrib>Shinde, Pramod B</creatorcontrib><creatorcontrib>Kim, Taewoon</creatorcontrib><creatorcontrib>Park, Seong-Min</creatorcontrib><creatorcontrib>Nimse, Satish Balasaheb</creatorcontrib><title>Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine</title><title>Biosensors (Basel)</title><addtitle>Biosensors (Basel)</addtitle><description>The measurement of cysteine in human urine and live cells is crucial for evaluating biological metabolism, monitoring and maintaining the immune system, preventing tissue/DNA damage caused by free radicals, preventing autoimmune diseases, and diagnosing disorders such as cystinuria and cancer. A method that uses a fluorescence turn-on probe and a portable fluorescence spectrometer device are crucial for highly sensitive, simple, rapid, and inexpensive cysteine detection. Herein, we present the synthesis and application of a benzimidazole-based fluorescent probe (
) along with the design and development of a portable fluorescence spectrometer device (
) for detecting cysteine in simulated human urine.
showed excellent selectivity and sensitivity in detecting cysteine over homocysteine, glutathione, and other amino acids with the response time of 1 min and demonstrated a detection limit of 16.3 nM using the developed
. Further,
also demonstrated its utility in detecting intracellular cysteine, making it an excellent probe for bio-imaging assay.</description><subject>Amino acids</subject><subject>Autoimmune diseases</subject><subject>Benzimidazoles</subject><subject>bio-imaging</subject><subject>Biomonitoring</subject><subject>biothiols</subject><subject>Chromatography</subject><subject>Cysteine</subject><subject>Cysteine - urine</subject><subject>Cystinuria</subject><subject>Damage prevention</subject><subject>DNA damage</subject><subject>Ethanol</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes</subject><subject>Fluorescent indicators</subject><subject>fluorimeter</subject><subject>Fluorometers</subject><subject>Free radicals</subject><subject>Glutathione</subject><subject>Homocysteine</subject><subject>Human wastes</subject><subject>Humans</subject><subject>Immune system</subject><subject>portable</subject><subject>Portable equipment</subject><subject>Response time</subject><subject>Selectivity</subject><subject>Spectrometry, Fluorescence</subject><subject>Spectrum analysis</subject><subject>Urine</subject><issn>2079-6374</issn><issn>2079-6374</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdkk1v1DAQhiMEolXpjTOyxIUDW_wVx7kg0S2llSpAAs7WxBm3XiX2Yicrtb8eb7ettszFM-NH73xoquotoydCtPRT52Nmxajk9EV1yGnTLpRo5Ms9_6A6znlFizWyaUXzujoQUnOmRH1Ybc5wg0NcjxgmEh0B8j2WBDnFcOdH38NdHHBxChl78jPFDgmE4sU0QTcgOR_mmPyIEybiYiLTDZKzEtnJx7DVW97mCX1A4gO5mEcI5E8q4ZvqlYMh4_HDe1T9Ov_6e3mxuPrx7XL55WphZaOnhW2salHRXtWNBg5W18LVvNNCW9a2TndWUsqc0h3tGYUt7aDGTnSN0uKoutyp9hFWZl0ahXRrInhzn4jp2kCavB3QCKipZEpzqp100Goq2ho6R6nVHJAXrc87rfXcjdjbsq8EwzPR5z_B35jruDFasbpWrAh8eBBI8e-MeTKjzxaHAQLGORuuKFeSc7Wt9f4_dBXnFMqi7qkyMZeyUB93lE0x54TuqRlGzfY6zP51FPzd_gBP8OMtiH-zC7W6</recordid><startdate>20211026</startdate><enddate>20211026</enddate><creator>Yeom, Gyu Seong</creator><creator>Song, In-Ho</creator><creator>Warkad, Shrikant Dashrath</creator><creator>Shinde, Pramod B</creator><creator>Kim, Taewoon</creator><creator>Park, Seong-Min</creator><creator>Nimse, Satish Balasaheb</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-5137-0584</orcidid><orcidid>https://orcid.org/0000-0002-3173-0896</orcidid></search><sort><creationdate>20211026</creationdate><title>Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine</title><author>Yeom, Gyu Seong ; Song, In-Ho ; Warkad, Shrikant Dashrath ; Shinde, Pramod B ; Kim, Taewoon ; Park, Seong-Min ; Nimse, Satish Balasaheb</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-c7c69e60d6578a2ac853f52b838c199f8bc4001f68b0d10ac69efa5eb3b7683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Amino acids</topic><topic>Autoimmune diseases</topic><topic>Benzimidazoles</topic><topic>bio-imaging</topic><topic>Biomonitoring</topic><topic>biothiols</topic><topic>Chromatography</topic><topic>Cysteine</topic><topic>Cysteine - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Biosensors (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yeom, Gyu Seong</au><au>Song, In-Ho</au><au>Warkad, Shrikant Dashrath</au><au>Shinde, Pramod B</au><au>Kim, Taewoon</au><au>Park, Seong-Min</au><au>Nimse, Satish Balasaheb</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine</atitle><jtitle>Biosensors (Basel)</jtitle><addtitle>Biosensors (Basel)</addtitle><date>2021-10-26</date><risdate>2021</risdate><volume>11</volume><issue>11</issue><spage>420</spage><pages>420-</pages><issn>2079-6374</issn><eissn>2079-6374</eissn><abstract>The measurement of cysteine in human urine and live cells is crucial for evaluating biological metabolism, monitoring and maintaining the immune system, preventing tissue/DNA damage caused by free radicals, preventing autoimmune diseases, and diagnosing disorders such as cystinuria and cancer. A method that uses a fluorescence turn-on probe and a portable fluorescence spectrometer device are crucial for highly sensitive, simple, rapid, and inexpensive cysteine detection. Herein, we present the synthesis and application of a benzimidazole-based fluorescent probe (
) along with the design and development of a portable fluorescence spectrometer device (
) for detecting cysteine in simulated human urine.
showed excellent selectivity and sensitivity in detecting cysteine over homocysteine, glutathione, and other amino acids with the response time of 1 min and demonstrated a detection limit of 16.3 nM using the developed
. Further,
also demonstrated its utility in detecting intracellular cysteine, making it an excellent probe for bio-imaging assay.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>34821635</pmid><doi>10.3390/bios11110420</doi><orcidid>https://orcid.org/0000-0002-5137-0584</orcidid><orcidid>https://orcid.org/0000-0002-3173-0896</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Biosensors (Basel), 2021-10, Vol.11 (11), p.420 |
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| language | eng |
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| source | Open Access: PubMed Central; Publicly Available Content Database (Proquest) (PQ_SDU_P3) |
| subjects | Amino acids Autoimmune diseases Benzimidazoles bio-imaging Biomonitoring biothiols Chromatography Cysteine Cysteine - urine Cystinuria Damage prevention DNA damage Ethanol Fluorescence Fluorescent Dyes Fluorescent indicators fluorimeter Fluorometers Free radicals Glutathione Homocysteine Human wastes Humans Immune system portable Portable equipment Response time Selectivity Spectrometry, Fluorescence Spectrum analysis Urine |
| title | Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine |
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