Production of Arachidonic Acid and Eicosapentaenoic Acid by Mortierella alpina CBS 528.72 on Date Waste

Arachidonic acid (ARA) and eicosapentaenoic acid (EPA) are important for human health and are produced by the fungus . Fermentation conditions may affect fatty acid profile of produced oil. Substrates in solid-state fermentation are usually renewable and low-cost agro-industrial waste. The aim of th...

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Bibliographic Details
Published in:Food technology and biotechnology 2018-04, Vol.56 (2), p.197-207
Main Authors: Asadi, Seyedeh Zeinab, Khosravi-Darani, Kianoush, Nikoopour, Houshang, Bakhoda, Hossein
Format: Article
Language:English
Subjects:
Agricultural wastes
Algae
Arachidonic acid
arachidonic acid (ARA)
Barley
Carbon
Carbon sources
Chemical properties
date waste
Design optimization
Eicosapentaenoic acid
eicosapentaenoic acid (EPA)
experimental design
Fatty acids
Fermentation
Fish oils
Food
Fungi
Industrial wastes
Linseed oil
Lipids
Microorganisms
Mortierella alpina
Nitrogen
Nitrogen sources
Omega 3 fatty acids
Original Scientific Papers
Process variables
Solid state
Solid state fermentation
Solids
Soybeans
Substrates
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Summary:Arachidonic acid (ARA) and eicosapentaenoic acid (EPA) are important for human health and are produced by the fungus . Fermentation conditions may affect fatty acid profile of produced oil. Substrates in solid-state fermentation are usually renewable and low-cost agro-industrial waste. The aim of this research is to study simultaneous production of ARA and EPA by CBS 528.72 in solid-state fermentation. Date waste and soybean meal served as carbon and nitrogen sources, respectively. Plackett-Burman design was used to evaluate the impact of eleven process variables on ARA and EPA production. Analysis of the results showed that the main variables with significant impact on production include seed age, and supplementation of linseed oil and nitrogen. These three variables were then studied in a central composite design for the optimization of production of both fatty acids. Finally, reconfirmation test was conducted to obtain experimental results from predicted optimum conditions. This report showed that simultaneous optimization of the production of ARA and EPA by CBS 528.72 on date waste can be achieved by modification of process variables in solid-state fermentation.
ISSN:1330-9862
1334-2606
DOI:10.17113/ftb.56.02.18.5379