Genetic Diversity Analysis of Rice Germplasm in Tripura State of Northeast India Using Drought and Blast Linked Markers

• Published in: Rice science 2017, Vol.24 (1), p.10-20
• Main Authors: Anupam, Alpana, Imam, Jahangir, Quatadah, Syed Mohammad, Siddaiah, Anantha, Prasad Das, Shankar, Variar, Mukund, Prasad Mandal, Nimai
• Format: Article
• Language: English
• Subjects: blast disease; drought; gene; genetic diversity; germplasm; molecular marker; quantitative trait locus; rice; 东北地区; 抗旱; 抗稻瘟病基因; 水稻种质; 种质资源; 稻瘟病抗性基因; 连锁标记; 遗传多样性分析
• ISSN: 1672-6308; 1876-4762
• DOI: 10.1016/j.rsci.2016.07.003

We genotyped 74 rice germplasms including Tripura＇s local landraces, improved varieties, cultivars and breeding lines and other rice varieties using molecular markers for genetic diversity, drought QTLs, and blast resistance genes. The number of alleles per locus ranged from 2 to 5 with an average of 2.9. The polymorphic information content value per locus ranged from 0.059（RM537） to 0.755（RM252） with an average of 0.475. Cluster analysis based on 30 simple sequence repeat markers revealed 5 clusters and also indicated the presence of variability within the rice accessions. The drought QTL q DTY2.1 was found in 56.0% of germplasms and q DTY1.1 was detected in only 6.8% of the germplasms. Out of seven rice blast resistance genes screened, only two rice varieties, RCPL-1-82 and Buh Vubuk（Lubuk）, were positive for four blast resistance genes while only Releng possessed two blast resistance genes. Among 74 rice germplasms, only three accessions, Releng, RCPL1-82 and Buh Vubuk（Lubuk）, possessed both drought-related QTLs and blast resistance genes. Overall, the 74 indigenous rice genotypes showed low level of genetic diversity, which is in contrast to high level of genetic diversity among rice varieties in northeast India, where highlights the good farming practice, conservation of germplasms and the limitation of molecular markers employed in this study. The presence of both drought related QTLs and blast resistance genes in some of the germplasms can be useful in future breeding programmes.