L-Arginine Inhibited Inflammatory Response and Oxidative Stress Induced by Lipopolysaccharide via Arginase-1 Signaling in IPEC-J2 Cells

This study aimed to explore the effect of L-arginine on lipopolysaccharide (LPS)-induced inflammatory response and oxidative stress in IPEC-2 cells. We found that the expression of toll-like receptor 4 ( ), myeloid differentiation primary response 88 ( ), cluster of differentiation 14 , nuclear fact...

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Bibliographic Details
Published in:International journal of molecular sciences 2019-04, Vol.20 (7), p.1800
Main Authors: Qiu, Yueqin, Yang, Xuefen, Wang, Li, Gao, Kaiguo, Jiang, Zongyong
Format: Article
Language:English
Subjects:
Apoptosis
Arginase
Arginase-1
Arginine
Bacteria
Cell cycle
Cell growth
Epithelial cells
Gram-negative bacteria
Immune response
Immune system
Inflammation
inflammatory effect
Inflammatory response
Innate immunity
Intestine
L-arginine
Lipid peroxidation
Lipids
lipopolysaccharide
Lipopolysaccharides
Mucosal immunity
Nitric oxide
Oxidative stress
Pathogens
Pattern recognition
Polyamines
Probiotics
Proteins
Salmonella
Studies
Swine
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Summary:This study aimed to explore the effect of L-arginine on lipopolysaccharide (LPS)-induced inflammatory response and oxidative stress in IPEC-2 cells. We found that the expression of toll-like receptor 4 ( ), myeloid differentiation primary response 88 ( ), cluster of differentiation 14 , nuclear factor-kappaBp65 ( ), chemokine-8 ( ), tumor necrosis factor ( and chemokine-6 ( ) mRNA were significantly increased by LPS. Exposure to LPS induced oxidative stress as reactive oxygen species (ROS) and malonaldehyde (MDA) production were increased while glutathione peroxidase (GSH-Px) were decreased in LPS-treated cells compared to those in the control. LPS administration also effectively induced cell growth inhibition through induction of G0/G1 cell cycle arrest. However, compared with the LPS group, cells co-treatment with L-arginine effectively increased cell viability and promoted the cell cycle into the S phase; L-arginine exhibited an anti-inflammatory effect in alleviating inflammation induced by LPS by reducing the abundance of , , , , and transcripts. Cells treated with LPS+L-arginine significantly enhanced the content of GSH-Px, while they decreased the production of ROS and MDA compared with the LPS group. Furthermore, L-arginine increased the activity of arginase-1 (Arg-1), while Arg-1 inhibitor abolished the protection of arginine against LPS-induced inflammation and oxidative stress. Taken together, these results suggested that L-arginine exerted its anti-inflammatory and antioxidant effects to protect IPEC-J2 cells from inflammatory response and oxidative stress challenged by LPS at least partly via the Arg-1 signaling pathway.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms20071800