Myostatin promotes distinct responses on protein metabolism of skeletal and cardiac muscle fibers of rodents

Myostatin is a novel negative regulator of skeletal muscle mass. Myostatin expression is also found in heart in a much less extent, but it can be upregulated in pathological conditions, such as heart failure. Myostatin may be involved in inhibiting protein synthesis and/or increasing protein degrada...

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Bibliographic Details
Published in:Brazilian journal of medical and biological research 2017-12, Vol.50 (12), p.e6733-e6733
Main Authors: Manfredi, L H, Paula-Gomes, S, Zanon, N M, Kettelhut, I C
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Cardiomyocytes
Cells, Cultured
Gene Expression
Health aspects
Male
Muscle Fibers, Skeletal - drug effects
Muscle Fibers, Skeletal - metabolism
Muscle Proteins - drug effects
Muscle Proteins - metabolism
Myocytes, Cardiac - drug effects
Myocytes, Cardiac - metabolism
Myostatin
Myostatin - pharmacology
Phosphorylation - drug effects
Phosphorylation - physiology
Protein degradation
Protein metabolism
Protein synthesis
Proteolysis - drug effects
Rats, Wistar
Real-Time Polymerase Chain Reaction
Reproducibility of Results
Rodents
Skeletal muscle
Time Factors
Tyrosine - drug effects
Tyrosine - metabolism
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Summary:Myostatin is a novel negative regulator of skeletal muscle mass. Myostatin expression is also found in heart in a much less extent, but it can be upregulated in pathological conditions, such as heart failure. Myostatin may be involved in inhibiting protein synthesis and/or increasing protein degradation in skeletal and cardiac muscles. Herein, we used cell cultures and isolated muscles from rats to determine protein degradation and synthesis. Muscles incubated with myostatin exhibited an increase in proteolysis with an increase of Atrogin-1, MuRF1 and LC3 genes. Extensor digitorum longus muscles and C2C12 myotubes exhibited a reduction in protein turnover. Cardiomyocytes showed an increase in proteolysis by activating autophagy and the ubiquitin proteasome system, and a decrease in protein synthesis by decreasing P70S6K. The effect of myostatin on protein metabolism is related to fiber type composition, which may be associated to the extent of atrophy mediated effect of myostatin on muscle.
ISSN:0100-879X
1414-431X
1414-431X
DOI:10.1590/1414-431X20176733