A Biomimetic Polynucleotides–Hyaluronic Acid Hydrogel Promotes Wound Healing in a Primary Gingival Fibroblast Model

Polynucleotides (PN) have long been known as an effective supportive therapy for wound healing. The present study investigated whether a hydrogel formulation containing PN and hyaluronic acid (PN + HA) could promote wound healing in an in vitro model of gingival fibroblasts. PN promoted cell growth...

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Bibliographic Details
Published in:Applied sciences 2021-05, Vol.11 (10), p.4405
Main Authors: Colangelo, Maria Teresa, Belletti, Silvana, Govoni, Paolo, Guizzardi, Stefano, Galli, Carlo
Format: Article
Language:English
Subjects:
Biomimetics
cell viability
Collagen
Dentistry
Extracellular matrix
Fibroblasts
gingiva
Hyaluronic acid
Hydrogels
Medical equipment
Nodules
Oral cavity
Polynucleotides
Proteins
Proteoglycans
Reagents
Soft tissues
Wound healing
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Summary:Polynucleotides (PN) have long been known as an effective supportive therapy for wound healing. The present study investigated whether a hydrogel formulation containing PN and hyaluronic acid (PN + HA) could promote wound healing in an in vitro model of gingival fibroblasts. PN promoted cell growth and viability as assessed by different assays, and PN + HA, though not significantly further increasing cell growth as compared to PN, supported the formation of dense multilayered cell nodules. PN promoted fibroblasts’ clonogenic efficiency and PN + HA further enhanced the formation of more numerous dense colonies. PN + HA appeared to significantly increase the expression of collagen 1a1 and 3a1, while not affecting proteoglycans deposition. Interestingly, when tested in a scratch assay, PN + HA achieved gap closure after 48 h, while cells in the comparison groups had not completely bridged the scratch even after 96 h. Taken together, these results demonstrate that PN + HA is a promising candidate for a supportive therapy to promote soft tissue healing in the oral cavity.
ISSN:2076-3417
2076-3417
DOI:10.3390/app11104405