Functional and Comparative Analysis of Two Subtypes of Cofilin Family on Cattle Myoblasts Differentiation

Agricultural meat composition and quality are not independent of the effects of skeletal muscle growth and development in animals. Cofilin is distributed extensively in muscle and non-muscle cells, and its function is tightly regulated in the cell. Cofilin has two variants in mammals, cofilin-1 (CFL...

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Published in:Agriculture (Basel) 2022-09, Vol.12 (9), p.1420
Main Authors: Sun, Yujia, Ma, Yaoyao, Wu, Xinyi, Zhao, Tianqi, Lu, Lu, Yang, Zhangping
Format: Article
Language:English
Subjects:
Animal genetics
Animal husbandry
Animal tissues
Beef cattle
Biomarkers
Cattle
Cofilin
cofilin-1
cofilin-2
Comparative analysis
Cytoskeleton
Deoxyribonucleic acid
Differentiation
DNA
DNA methylation
expression pattern
Fetuses
Gene expression
Genes
Genetics
Laboratories
Mammals
Meat quality
Methylation
mRNA
Muscles
Musculoskeletal system
myoblast differentiation
Myoblasts
MyoD protein
Physiology
Polymerization
Proteins
Reagents
RNA
Skeletal muscle
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Summary:Agricultural meat composition and quality are not independent of the effects of skeletal muscle growth and development in animals. Cofilin is distributed extensively in muscle and non-muscle cells, and its function is tightly regulated in the cell. Cofilin has two variants in mammals, cofilin-1 (CFL1, non-muscle type) and cofilin-2 (CFL2, muscle type), and has a dual function on skeletal muscle fibers. Our study examined the expression pattern of CFL1 and CFL2 in different fetal bovine, calf, and adult cattle tissues. The content of the CFL2 gene increased significantly with the increase in cattle age in muscle tissues; CFL1 showed the opposite trend. In muscle tissues, DNA methylation levels of CFL1 and CFL2 were high in fetal bovine, and the mRNA level of CFL2 was significantly lower compared to CFL1. However, DNA methylation levels of CFL2 were lower than CFL1, and the mRNA level of CFL2 was remarkably higher compared to CFL1 in adult cattle. Overexpression of CFL1 or knockdown CFL2 reduced the expression levels of muscle differentiation markers, i.e., MYOD, MYOG, and MYH3. Overexpression of CFL2 or knockdown CFL1 stimulated the expression of these marker genes. Therefore, CFL2 may be superior to CFL1 as a candidate gene for subsequent research on cattle genetics and breeding.
ISSN:2077-0472
2077-0472
DOI:10.3390/agriculture12091420