Potential roles of melatonin and ABA on apple dwarfing in semi-arid area of Xinjiang China

• Published in: PeerJ (San Francisco, CA) CA), 2022-03, Vol.10, p.e13008-e13008, Article e13008
• Main Authors: Yan, Tianci, Mei, Chuang, Song, Handong, Shan, Dongqian, Sun, Yanzhao, Hu, Zehui, Wang, Lin, Zhang, Tong, Wang, Jixun, Kong, Jin
• Format: Article
• Language: English
• Subjects: Abscisic acid; Abscisic acid (ABA); Abscisic Acid - metabolism; Agricultural Science; Apple; Biochemistry; Biosynthesis; Brassinolide; Cell division; Cytokinins; Drought; Drought stress; Dwarfing; Enzymes; Experiments; Genes; Germplasm; Gibberellins; Gibberellins - metabolism; Hormones; Indoleacetic acid; Malus - genetics; Melatonin; Melatonin (MT); Molecular Biology; Orchards; Phenotypes; Plant Breeding; Plant growth; Plant Science; Variance analysis; Zeatin
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.13008

Dwarfing is a typic breeding trait for mechanical strengthening and relatively high yield in modern apple orchards. Clarification of the mechanisms associated with dwarfing is important for use of molecular technology to breed apple. Herein, we identified four dwarfing apple germplasms in semi-arid area of Xinjiang, China. The internodal distance of these four germplasms were significantly shorter than non-dwarfing control. Their high melatonin (MT) contents are negatively associated with their malondialdehyde (MDA) levels and oxidative damage. In addition, among the detected hormones including auxin (IAA), gibberellin (GA), brassinolide (BR), zeatin-riboside (ZR), and abscisic acid (ABA), only ABA and ZR levels were in good correlation with the dwarfing phenotype. The qPCR results showed that the expression of melatonin synthetic enzyme genes and , ABA synthetic enzyme gene and degradative gene , ZR synthetic enzyme gene all correlated well with the enhanced levels of MT, ABA and the reduced level of of ZR in the dwarfing germplasms. Furthermore, the significantly higher expression of ABA marker genes ( and ) and the lower expression of ZR marker genes ( and ) in all the four dwarf germplasms were consistent with the ABA and ZR levels. Considering the yearly long-term drought occurring in Xinjiang, China, it seems that dwarfing with high contents of MT and ABA may be a good strategy for these germplasms to survive against drought stress. This trait of dwarfing may also benefit apple production and breeding in this semi-arid area.