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Phthalates and phthalate metabolites in urine from Tianjin and implications for platelet mitochondrial DNA methylation
Phthalates (PAEs) are important synthetic substances in plastics, attracting much attention due to their potential effects on the cardiovascular system. In this study, urine and blood samples from 39 individuals were collected in Tianjin, China. Phthalates and phthalate metabolites (mPAEs) were anal...
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Published in: | Frontiers in public health 2023-04, Vol.11, p.1108555-1108555 |
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Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Phthalates (PAEs) are important synthetic substances in plastics, attracting much attention due to their potential effects on the cardiovascular system.
In this study, urine and blood samples from 39 individuals were collected in Tianjin, China. Phthalates and phthalate metabolites (mPAEs) were analyzed using gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography-mass spectrometry (HPLC-MS), respectively. The polymerase chain reaction (PCR) products from bisulfite-treated mitochondrial DNA (
) samples were analyzed using pyrosequencing technology.
The detection frequencies for 9 PAEs varied from 2.56 to 92.31%, and those for 10 mPAEs varied from 30.77 to 100%. The estimated daily intakes (EDIs) and cumulative risk of PAEs were calculated based on the experimental statistics of urinary PAEs and mPAEs. For PAEs, the HI
(hazard index corresponding to reference doses) values of 10.26% of participants and the HI
(hazard index corresponding to tolerable daily intake) values of 30.77% of participants were estimated to exceed 1, suggesting a relatively high exposure risk. The
methylation levels in the
and
were observed to be lower than in the
. Mono-ethyl phthalate (MEP) and
were positively correlated with triglyceride levels (
< 0.05). Based on the association of PAEs,
methylation, and triglycerides, the mediating role of
methylation between PAEs and cardiovascular diseases (CVDs) was analyzed in this study, but no mediated effect was observed.
The effects of PAE exposure on cardiovascular diseases (CVDs) should be investigated further. |
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ISSN: | 2296-2565 2296-2565 |
DOI: | 10.3389/fpubh.2023.1108555 |