In vivo bioluminescent imaging (BLI): noninvasive visualization and interrogation of biological processes in living animals

In vivo bioluminescent imaging (BLI) is increasingly being utilized as a method for modern biological research. This process, which involves the noninvasive interrogation of living animals using light emitted from luciferase-expressing bioreporter cells, has been applied to study a wide range of bio...

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Bibliographic Details
Published in:Sensors 2011-01, Vol.11 (1), p.180-206
Main Authors: Close, Dan M, Xu, Tingting, Sayler, Gary S, Ripp, Steven
Format: Article
Language:English
Subjects:
Animals
bacterial luciferase
bioluminescent imaging
Biosensing Techniques
BLI
firefly luciferase
Genes, Reporter
Luc
Luciferases - genetics
Luciferases - metabolism
Luminescent Measurements
Lux
Models, Animal
Review
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Summary:In vivo bioluminescent imaging (BLI) is increasingly being utilized as a method for modern biological research. This process, which involves the noninvasive interrogation of living animals using light emitted from luciferase-expressing bioreporter cells, has been applied to study a wide range of biomolecular functions such as gene function, drug discovery and development, cellular trafficking, protein-protein interactions, and especially tumorigenesis, cancer treatment, and disease progression. This article will review the various bioreporter/biosensor integrations of BLI and discuss how BLI is being applied towards a new visual understanding of biological processes within the living organism.
ISSN:1424-8220
1424-8220
DOI:10.3390/s110100180