Extracting DNA of nematodes communities from Argentine Pampas agricultural soils

We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation experim...

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Bibliographic Details
Published in:Anais da Academia Brasileira de Ciências 2015-06, Vol.87 (2), p.691-697
Main Authors: Mondino, Eduardo A, Covacevich, Fernanda, Studdert, Guillermo A, Pimentel, João P, Berbara, Ricardo L L
Format: Article
Language:English
Subjects:
Agriculture
Agroecossistemas
Animals
Argentina
CTAB
DNA, Helminth - isolation & purification
extra ção de ácido nucleico
MULTIDISCIPLINARY SCIENCES
Nematoda - genetics
nematóides
Polymerase Chain Reaction
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Summary:We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful method because positive amplifications were obtained by using both eukaryotic and nematode specific primers. This work could contribute to biodiversity studies of nematodes on agroecosystems.
ISSN:0001-3765
1678-2690
1678-2690
0001-3765
DOI:10.1590/0001-3765201520130110