Comparative transcriptomics of spotted seatrout (Cynoscion nebulosus) populations to cold and heat stress

• Published in: Ecology and evolution 2021-02, Vol.11 (3), p.1352-1367
• Main Authors: Song, Jingwei, McDowell, Jan R.
• Format: Article
• Language: English
• Subjects: Apoptosis; Climate change; Colds; Cynoscion nebulosus; Estuaries; Experiments; Fish; Gene expression; Gene regulation; Gene sequencing; Genes; GRP78 protein; Heat; Heat stress; Heat waves; Immune response; Liver; Metabolism; Original Research; Phenotypic plasticity; Physiology; Plastic properties; Plasticity; Population; Population control; Ribonucleic acid; RNA; RNA‐seq; Sample size; Stress response; Temperature gradients; temperature stress; Transcription; transcriptome; Transcriptomics; Water temperature
• ISSN: 2045-7758; 2045-7758
• DOI: 10.1002/ece3.7138

Resilience to climate change depends on a species' adaptive potential and phenotypic plasticity. The latter can enhance survival of individual organisms during short periods of extreme environmental perturbations, allowing genetic adaptation to take place over generations. Along the U.S. East Coast, estuarine‐dependent spotted seatrout (Cynoscion nebulosus) populations span a steep temperature gradient that provides an ideal opportunity to explore the molecular basis of phenotypic plasticity. Genetically distinct spotted seatrout sampled from a northern and a southern population were exposed to acute cold and heat stress (5 biological replicates in each treatment and control group), and their transcriptomic responses were compared using RNA‐sequencing (RNA‐seq). The southern population showed a larger transcriptomic response to acute cold stress, whereas the northern population showed a larger transcriptomic response to acute heat stress compared with their respective population controls. Shared transcripts showing significant differences in expression levels were predominantly enriched in pathways that included metabolism, transcriptional regulation, and immune response. In response to heat stress, only the northern population significantly upregulated genes in the apoptosis pathway, which could suggest greater vulnerability to future heat waves in this population as compared to the southern population. Genes showing population‐specific patterns of expression, including hpt, acot, hspa5, and hsc71, are candidates for future studies aiming to monitor intraspecific differences in temperature stress responses in spotted seatrout. Our findings contribute to the current understanding of phenotypic plasticity and provide a basis for predicting the response of a eurythermal fish species to future extreme temperatures. Spotted seatrout, Cynoscion nebulosus, is a popular saltwater sport fish in the United States, and distinct populations exist along the US east coast. We de novo assembled the first high‐quality liver transcriptome of the species and discovered both shared and unique transcriptomic responses after cold and heat stress in fish with distinct genetic background.