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Characterization of a Type VI Secretion System vgrG2 Gene in the Pathogenicity of Burkholderia thailandensis BPM
is a clinically underestimated conditional pathogen in the genus , the pathogenicity of the infection caused by remains poorly understood. According to previous studies, Type-VI secretion system (T6SS) is a protein secreting device widely existing in Gram-negative bacilli. Valine-glycine repeat prot...
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Published in: | Frontiers in microbiology 2022-01, Vol.12, p.811343-811343 |
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Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | is a clinically underestimated conditional pathogen in the genus
, the pathogenicity of the infection caused by
remains poorly understood. According to previous studies, Type-VI secretion system (T6SS) is a protein secreting device widely existing in Gram-negative bacilli. Valine-glycine repeat protein G (VgrG) is not only an important component of T6SS, but also a virulence factor of many Gram-negative bacilli. In one of our previous studies, a unique T6SS
gene (
gene) was present in a virulent
strain BPM (BPM), but not in the relatively avirulent
strain E264 (E264). Meanwhile, transcriptome analysis of BPM and E264 showed that the
gene was strongly expressed in BPM, but not in E264. Therefore, we identified the function of the
gene by constructing the mutant and complemented strains in this study.
, the
gene was observed to be involved in the interactions with host cells. The animal model experiment showed that the deletion of
gene significantly led to the decrease in the lethality of BPM and impaired its ability to trigger host immune response. In conclusion, our study provides a new perspective for studying the pathogenicity of
and lays the foundation for discovering the potential T6SS effectors. |
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ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2021.811343 |