A simple method for human peripheral blood monocyte isolation

We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient...

Full description

Saved in:
Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2000-04, Vol.95 (2), p.221-223
Main Authors: de Almeida, M C, Silva, A C, Barral, A, Barral Netto, M
Format: Article
Language:English
Subjects:
Cell Separation - methods
Centrifugation, Density Gradient - methods
gradient-isolation
Humans
Monocytes
PARASITOLOGY
percoll
TROPICAL MEDICINE
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.
ISSN:0074-0276
1678-8060
0074-0276
1678-8060
DOI:10.1590/S0074-02762000000200014