Antioxidant, Antidiabetic, Anticholinergic, and Antiglaucoma Effects of Magnofluorine

• Published in: Molecules (Basel, Switzerland) Switzerland), 2022-09, Vol.27 (18), p.5902
• Main Authors: Durmaz, Lokman, Kiziltas, Hatice, Guven, Leyla, Karagecili, Hasan, Alwasel, Saleh, Gulcin, İlhami
• Format: Article
• Language: English
• Subjects: acetylcholinesterase; Acetylcholinesterase - metabolism; Acids; alpha-Tocopherol - pharmacology; Alzheimer's disease; Analysis; Anticholinergics; Antidiabetics; antioxidant activity; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Aporphines - pharmacology; BHA; Biphenyl Compounds; Butylated Hydroxyanisole - pharmacology; Butylated Hydroxytoluene - pharmacology; butyrylcholinesterase; Butyrylcholinesterase - metabolism; Carbonic anhydrase; Carbonic Anhydrase II; Cholinergic Antagonists; Cholinesterase Inhibitors - chemistry; Cholinesterase Inhibitors - pharmacology; Diabetes mellitus; Enzymes; Free radicals; Glaucoma; Glycosidases; Glycoside Hydrolases; Humans; Hypoglycemic Agents - pharmacology; Industrial applications; Magnofluorine; Metabolites; Molecular docking; Molecular Docking Simulation; Oxidative stress; phenolic compound; Phenylenediamine; Picrates; Potassium ferricyanide; Scavenging; Sulfonic acid; Sulfonic Acids - chemistry; Tocopherol; Tocopherols
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27185902

Magnofluorine, a secondary metabolite commonly found in various plants, has pharmacological potential; however, its antioxidant and enzyme inhibition effects have not been investigated. We investigated the antioxidant potential of Magnofluorine using bioanalytical assays with 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS ), , -dimethyl- -phenylenediamine dihydrochloride (DMPD ), and 1,1-diphenyl-2-picrylhydrazyl (DPPH ) scavenging abilities and K [Fe(CN) ] and Cu reduction abilities. Further, we compared the effects of Magnofluorine and butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), α-Tocopherol, and Trolox as positive antioxidant controls. According to the analysis results, Magnofluorine removed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals with an IC value of 10.58 μg/mL. The IC values of BHA, BHT, Trolox, and α-Tocopherol were 10.10 μg/mL, 25.95 μg/mL, 7.059 μg/mL, and 11.31 μg/mL, respectively. Our results indicated that the DPPH· scavenging effect of Magnofluorine was similar to that of BHA, close to that of Trolox, and better than that of BHT and α-tocopherol. The inhibition effect of Magnofluorine was examined against enzymes, such as acetylcholinesterase (AChE), α-glycosidase, butyrylcholinesterase (BChE), and human carbonic anhydrase II (hCA II), which are linked to global disorders, such as diabetes, Alzheimer's disease (AD), and glaucoma. Magnofluorine inhibited these metabolic enzymes with Ki values of 10.251.94, 5.991.79, 25.411.10, and 30.563.36 nM, respectively. Thus, Magnofluorine, which has been proven to be an antioxidant, antidiabetic, and anticholinergic in our study, can treat glaucoma. In addition, molecular docking was performed to understand the interactions between Magnofluorine and target enzymes BChE (D: 6T9P), hCA II (A:3HS4), AChE (B:4EY7), and α-glycosidase (C:5NN8). The results suggest that Magnofluorine may be an important compound in the transition from natural sources to industrial applications, especially new drugs.