Using core genome multilocus sequence typing (cgMLST) for vancomycin-resistant Enterococcus faecium isolates to guide infection control interventions and end an outbreak

• Published in: Journal of global antimicrobial resistance. 2021-03, Vol.24, p.418-423
• Main Authors: Kjær Hansen, Sanne, Andersen, Lise, Detlefsen, Mette, Holm, Anette, Roer, Louise, Antoniadis, Panagiotis, Skov, Marianne Nielsine, Hammerum, Anette M., Kemp, Michael
• Format: Article
• Language: English
• Subjects: cgMLST; Epidemiology; Infection control; Outbreak; Transmission; VRE
• ISSN: 2213-7165; 2213-7173
• DOI: 10.1016/j.jgar.2021.02.007

•cgMLST is a useful tool to reveal transmission of VRE in and between hospitals.•cgMLST delineates infection control interventions in local outbreak investigations.•ST80-CT993 vanA E. faecium outbreak in a Danish university hospital. Until July 2016, vancomycin-resistantEnterococcus faecium (VREfm) was sporadically detected in Odense University Hospital, Denmark. After July 2016, the number of VREfm cases increased. This study aimed to apply a core genome multilocus sequence typing (cgMLST) scheme for E. faecium to type and analyse VREfm isolates collected at a single Danish hospital and to compare the results with cgMLST data from other regions of Denmark to trace transmission. A total of 38 VREfm clinical isolates from inpatients at the hospital in the period January 2014 through June 2017 were included in the study and analysed using whole-genome sequencing. Use of SeqSphere + software was initiated from the beginning of June 2017 to obtain MLST, cgMLST and epi curves. Admission histories were incorporated and national surveillance data on cgMLST were used to identify transmission routes. Six different sequence types (STs) were identified, the most frequent being ST80, ST117 and ST203. cgMLST subdivided the 38 isolates into 18 different complex types (CTs) with 13 isolates (34%) belonging to ST80-CT993. Epi curves indicated transmission of ST80-CT993 in several departments. Transmission from patients transferred from other hospitals was not identifiable. Infection control interventions launched in one department ended the outbreak. The high resolution of cgMLST allowed for detailed interpretation with evidence of nosocomial transmission of specific CTs. cgMLST made it easy to compare our local isolates with national findings, thereby clarifying transmission routes. Supplemented with admission histories, cgMLST targeted the epidemiological investigation and delineated the expensive and time-consuming infection control interventions.