Co-culture of Bacillus amyloliquefaciens ACCC11060 and Trichoderma asperellum GDFS1009 enhanced pathogen-inhibition and amino acid yield

• Published in: Microbial cell factories 2018-10, Vol.17 (1), p.155-155, Article 155
• Main Authors: Wu, Qiong, Ni, Mi, Dou, Kai, Tang, Jun, Ren, Jianhong, Yu, Chuanjin, Chen, Jie
• Format: Article
• Language: English
• Subjects: Amino acid; Amino acids; Amino Acids - metabolism; Antibiosis; Antiinfectives and antibacterials; Antimicrobial agents; Ascomycota; Bacillus; Bacillus amyloliquefaciens; Bacillus amyloliquefaciens - metabolism; Bacteria; Biocontrol metabolites; Biological control; Cell culture; Chemical compounds; Chromatography, Liquid - methods; Coculture Techniques; Cultivation; Fermentation; Fungi; Growth; Innovations; Inoculation; Liquid chromatography; Liquid co-cultivation; Liquor; Mass spectrometry; Mass spectroscopy; Metabolites; Morphology; Plant resistance; Scientific imaging; Spectroscopy; Trichoderma - metabolism; Trichoderma asperellum
• ISSN: 1475-2859; 1475-2859
• DOI: 10.1186/s12934-018-1004-x

Bacillus spp. are a genus of biocontrol bacteria widely used for antibiosis, while Trichoderma spp. are biocontrol fungi that are abundantly explored. In this study, a liquid co-cultivation of these two organisms was tried firstly. Through liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS), it was discovered that with an inoculation in the ratio of 1.9:1, the antimicrobial effect of the co-cultured fermentation liquor of Bacillus amyloliquefaciens ACCC11060 and Trichoderma asperellum GDFS1009 was found to be significantly higher than that of pure-cultivation. A raise in the synthesis of antimicrobial substances contributed to this significant increase. Additionally, a co-culture with the inoculation of the two organisms in the ratio of 1:1 was found to enhance the production of specific amino acids. This technique could be further explored for either a large scale production of amino acids or could serve as a theoretical base for the generation of certain rare amino acids. This work clearly demonstrated that co-cultivation of B. amyloliquefaciens ACCC11060 and T. asperellum GDFS1009 could produce more specific biocontrol substances and amino acids.