Metabolites of Medicarpin and Their Distributions in Rats

Medicarpin is a bioactive pterocarpan that has been attracting increasing attention in recent years. However, its metabolic fate in vivo is still unknown. To clarify its metabolism and the distribution of its metabolites in rats after oral administration, the HPLC-ESI-IT-TOF-MS technique was used. A...

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Published in:Molecules (Basel, Switzerland) Switzerland), 2019-05, Vol.24 (10), p.1966
Main Authors: Wang, Hong-Yan, Li, Teng, Ji, Rui, Xu, Feng, Liu, Guang-Xue, Li, Yao-Li, Shang, Ming-Ying, Cai, Shao-Qing
Format: Article
Language:English
Subjects:
Adipocytes
Animals
Ascorbic acid
Colon
Conjugation
Demethylation
distribution
Glycosylation
High-performance liquid chromatography
HPLC-ESI-IT-TOF-MSn
Hydrogenation
Hydroxylation
in vivo
In vivo methods and tests
Information retrieval
Intestine
Isomerization
Kinases
Liquid chromatography
medicarpin
Metabolism
Metabolites
Methylation
Oral administration
Organs
pterocarpan
R&D
Research & development
Spleen
Sulfation
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Summary:Medicarpin is a bioactive pterocarpan that has been attracting increasing attention in recent years. However, its metabolic fate in vivo is still unknown. To clarify its metabolism and the distribution of its metabolites in rats after oral administration, the HPLC-ESI-IT-TOF-MS technique was used. A total of 165 new metabolites (13 phase I and 152 phase II metabolites) were tentatively identified, and 104, 29, 38, 41, 74, 28, 24, 15, 42, 8, 10, 3, and 17 metabolites were identified in urine, feces, plasma, the colon, intestine, stomach, liver, spleen, kidney, lung, heart, brain, and thymus, respectively. Metabolic reactions included demethylation, hydrogenation, hydroxylation, glucuronidation, sulfation, methylation, glycosylation, and vitamin C conjugation. (medicarpin glucuronide), (vestitol-1'- -glucuronide) were distributed to 10 organs, and was the most abundant metabolite in seven organs. Moreover, we found that isomerization of medicarpin must occur in vivo. At least 93 metabolites were regarded as potential new compounds by retrieving information from the Scifinder database. This is the first detailed report on the metabolism of ptercarpans in animals, which will help to deepen the understanding of the metabolism characteristics of medicarpin in vivo and provide a solid basis for further studies on the metabolism of other pterocarpans in animals.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules24101966