Different Expressions of Pericardial Fluid MicroRNAs in Patients With Arrhythmogenic Right Ventricular Cardiomyopathy and Ischemic Heart Disease Undergoing Ventricular Tachycardia Ablation

Pericardial fluid is enriched with biologically active molecules of cardiovascular origin including microRNAs. Investigation of the disease-specific extracellular microRNAs could shed light on the molecular processes underlying disease development. Arrhythmogenic right ventricular cardiomyopathy (AR...

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Bibliographic Details
Published in:Frontiers in cardiovascular medicine 2021-03, Vol.8, p.647812-647812
Main Authors: Khudiakov, Aleksandr A, Panshin, Daniil D, Fomicheva, Yulia V, Knyazeva, Anastasia A, Simonova, Ksenia A, Lebedev, Dmitry S, Mikhaylov, Evgeny N, Kostareva, Anna A
Format: Article
Language:English
Subjects:
arrhythmogenic (right ventricular) cardiomyopathy
Cardiovascular Medicine
expression analyses
Medicin och hälsovetenskap
microRNA
pericardial fluid
small RNA sequencing
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Summary:Pericardial fluid is enriched with biologically active molecules of cardiovascular origin including microRNAs. Investigation of the disease-specific extracellular microRNAs could shed light on the molecular processes underlying disease development. Arrhythmogenic right ventricular cardiomyopathy (ARVC) is an inherited heart disease characterized by life-threatening arrhythmias and progressive heart failure development. The current data about the association between microRNAs and ARVC development are limited. We performed small RNA sequence analysis of microRNAs of pericardial fluid samples obtained during transcutaneous epicardial access for ventricular tachycardia (VT) ablation of six patients with definite ARVC and three post-infarction VT patients. Disease-associated microRNAs of pericardial fluid were identified. Five microRNAs (hsa-miR-1-3p, hsa-miR-21-5p, hsa-miR-122-5p, hsa-miR-206, and hsa-miR-3679-5p) were found to be differentially expressed between patients with ARVC and patients with post-infarction VT. Enrichment analysis of differentially expressed microRNAs revealed their close linkage to cardiac diseases. Our data extend the knowledge of pericardial fluid microRNA composition and highlight five pericardial fluid microRNAs potentially linked to ARVC pathogenesis. Further studies are required to confirm the use of pericardial fluid RNA sequencing in differential diagnosis of ARVC.
ISSN:2297-055X
2297-055X
DOI:10.3389/fcvm.2021.647812