Identification of Potential Core Genes Between Primary and Metastatic Malignant Melanoma and Analysis of Their Immune Correlation

To identify the potential differential genes between primary and metastatic melanoma, screen out immune-related genes in core genes and analyze their immune correlation, thus searching for the early diagnostic biomarkers of cutaneous malignant melanoma (CMM) and the targets of curbing metastasis. We...

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Bibliographic Details
Published in:International journal of general medicine 2022, Vol.15, p.379-391
Main Authors: Jia, Cong-Li, Yang, Fu, Li, Rui-Ning
Format: Article
Language:English
Subjects:
Bioinformatics
Biomarkers
Cancer therapies
cdh1
Cell adhesion & migration
Connectivity
cutaneous malignant melanoma
Diagnosis
dsg1
egfr
Epidermal growth factor
Extracellular matrix
flg
Gene expression
Genes
Genomes
Genomics
Keratin
Medical prognosis
Medical research
Medicine, Experimental
Melanoma
Metastasis
Original Research
pkp1
Protein-protein interactions
Proteins
Skin cancer
Survival analysis
Tumors
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Summary:To identify the potential differential genes between primary and metastatic melanoma, screen out immune-related genes in core genes and analyze their immune correlation, thus searching for the early diagnostic biomarkers of cutaneous malignant melanoma (CMM) and the targets of curbing metastasis. We analyzed two microarray datasets (GSE8401 and GSE46517) derived from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) between primary and metastatic melanoma were screened out using the GEO2R tool. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed to identify the functions and pathways of DEGs. We analyzed protein-protein interaction of these DEGs based on the Search Tool for the Retrieval of Interacting Genes database and showed by Cytoscape software. In addition, the online Gene Expression Profiling Interactive Analysis tool (GEPIA) was used to analyze the prognostic value of hub genes expressed in metastatic melanoma patients. Immune-related genes in hub genes were screened and further analyzed. A total of 178 upregulated DEGs and 4 downregulated DEGs were identified. 23 terms and 4 pathways were confirmed related to metastatic melanoma. Ten hub genes with a high degree of connectivity were found. Overexpression of three hub genes (DSG1, FLG, PKP1) (P
ISSN:1178-7074
1178-7074
DOI:10.2147/IJGM.S338890