Beneficial Effect of Gastrodia elata Blume and Poria cocos Wolf Administration on Acute UVB Irradiation by Alleviating Inflammation through Promoting the Gut-Skin Axis

• Published in: International journal of molecular sciences 2022-09, Vol.23 (18), p.10833
• Main Authors: Zhang, Ting, Huang, Shaokai, Qiu, Jingyi, Wu, Xuangao, Yuan, Heng, Park, Sunmin
• Format: Article
• Language: English
• Subjects: Acne; Agaricales; Animals; Bioactive compounds; Biosynthesis; Body weight; Butanediol; Butylene Glycols; Cellulose; Chinese medicine; Cytokines; Dietary fiber; Digestive system; Erythema; Exposure; Fecal microflora; Flavonoids; Gastrodia; Gastrodia elata Blume; Gene expression; Hypotheses; Inflammation; Inflammation - drug therapy; Interleukin 13; Interleukin 4; Intestine; Intestines; Lipopolysaccharides; Malondialdehyde; Mast cells; Metabolism; Metabolites; metagenome analysis; Mice; Mice, Inbred ICR; Microbiota; Oxidative stress; photoaging; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Polyphenols; Poria cocos; Propionates; Propionic acid; Retinoic acid; RNA, Messenger; Skin; Skin cancer; Skin diseases; Skin lesions; Tretinoin; Tumor Necrosis Factor-alpha - genetics; Tumor necrosis factor-α; Ultraviolet radiation; Ultraviolet Rays; UVB; Water; Wolfiporia
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms231810833

Bioactive compounds in some herbs can, directly and indirectly, protect against photoaging. We evaluated the effects of Blume (GE) and Wolf (PC) water extracts on ultraviolet (UV) B-induced skin lesions by acute UVB exposure in ICR mice and explored their mechanism of action. After removing the hair on the back of the mice, UVB (280-310 nm) was exposed to the back for 30 min to induce skin damage. Four UVB exposure groups were divided into the following according to the local application (1,3-butanediol extract) on the dorsal skin and oral intake (0.3 g water extract/kg body weight/day): 1,3-butanediol and cellulose(control; UV-Con), retinoic acid (positive-control; UV-Positive), PC extracts (UV-PC), and GE extracts (UV-GE). The fifth group had no UVB exposure with the same treatment as the UV-Con (Normal-control). The erythema, burns, erosion, and wounds of the UV-PC and UV-PC groups were alleviated, and the most significant improvements occurred in the UV-PC group. PC and GE reduced the thickness of the dorsal skin tissue, the penetration of mast cells, and malondialdehyde contents. The mRNA expression of , , and , inflammatory factors, were also reduced significantly in the dorsal skin of the UV-PC and UV-GE groups. UV-PC, UV-GE, and UV-Positive showed improvements in UV-induced intestinal tissue inflammation. UV-Con deteriorated the intestinal morphology, and PC and GE alleviated it. The α-diversity of the fecal microbiota decreased in the UV-control, and UV-PC and UV-GE prevented the decrease. Fecal metagenome analysis revealed increased propionate biosynthesis in the UV-PC group but decreased lipopolysaccharide biosynthesis in the UV-PC and UV-GE groups compared to UV-Con. In conclusion, the local application and intake of PC and GE had significant therapeutic effects on acute UV-induced skin damage by reducing oxidative stress and proinflammatory cytokines, potentially promoting the gut-microbiota-gut-skin axis.