Raman Microspectroscopic Analysis of Selenium Bioaccumulation by Green Alga Chlorella vulgaris

Selenium (Se) is an element with many commercial applications as well as an essential micronutrient. Dietary Se has antioxidant properties and it is known to play a role in cancer prevention. However, the general population often suffers from Se deficiency. Green algae, such as , cultivated in Se-en...

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Bibliographic Details
Published in:Biosensors (Basel) 2021-04, Vol.11 (4), p.115
Main Authors: Kizovský, Martin, Pilát, Zdeněk, Mylenko, Mykola, Hrouzek, Pavel, Kuta, Jan, Skoupý, Radim, Krzyžánek, Vladislav, Hrubanová, Kamila, Adamczyk, Olga, Ježek, Jan, Bernatová, Silvie, Klementová, Tereza, Gjevik, Alžběta, Šiler, Martin, Samek, Ota, Zemánek, Pavel
Format: Article
Language:English
Subjects:
Algae
Amino acids
Antioxidants
Aquatic plants
Bioaccumulation
Biomass
Chlorella
Chlorella vulgaris
Chlorella vulgaris - chemistry
Chlorella vulgaris - metabolism
Chromatography
Diet
Dietary supplements
EDX
Enrichment
Food
ICP-MS
Inductively coupled plasma mass spectrometry
Mass spectrometry
Mass spectroscopy
Raman spectroscopy
Scientific imaging
Selenium
Selenium - analysis
Selenium - metabolism
Spectrum Analysis, Raman
Water Pollutants, Chemical - analysis
Water Pollutants, Chemical - metabolism
X ray imagery
X-ray spectroscopy
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Summary:Selenium (Se) is an element with many commercial applications as well as an essential micronutrient. Dietary Se has antioxidant properties and it is known to play a role in cancer prevention. However, the general population often suffers from Se deficiency. Green algae, such as , cultivated in Se-enriched environment may be used as a food supplement to provide adequate levels of Se. We used Raman microspectroscopy (RS) for fast, reliable, and non-destructive measurement of Se concentration in living algal cells. We employed inductively coupled plasma-mass spectrometry as a reference method to RS and we found a substantial correlation between the Raman signal intensity at 252 cm and total Se concentration in the studied cells. We used RS to assess the uptake of Se by living and inactivated algae and demonstrated the necessity of active cellular transport for Se accumulation. Additionally, we observed the intracellular Se being transformed into an insoluble elemental form, which we further supported by the energy-dispersive X-ray spectroscopy imaging.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios11040115