Oxidative stress activates NORAD expression by H3K27ac and promotes oxaliplatin resistance in gastric cancer by enhancing autophagy flux via targeting the miR-433-3p

• Published in: Cell death & disease 2021-01, Vol.12 (1), p.90-90, Article 90
• Main Authors: Wang, Jizhao, Sun, Yuchen, Zhang, Xing, Cai, Hui, Zhang, Cheng, Qu, Hangying, Liu, Lin, Zhang, Mingxin, Fu, Junke, Zhang, Jia, Wang, Jiansheng, Zhang, Guangjian
• Format: Article
• Language: English
• Subjects: 13/1; 13/2; 14/19; 14/28; 14/32; 38/109; 38/77; 631/67/1504/1829; 631/67/395; 64/60; Antibodies; Apoptosis; Autophagy; Biochemistry; Biomedical and Life Sciences; Cell Biology; Cell Culture; Cholecystokinin; DNA damage; DNA repair; Enzyme-linked immunosorbent assay; Flow cytometry; Gastric cancer; Immunology; Life Sciences; Oxaliplatin; Oxidative stress; Phagocytosis; Reporter gene; Ribonucleic acid; RNA; Western blotting
• ISSN: 2041-4889; 2041-4889
• DOI: 10.1038/s41419-020-03368-y

Oxaliplatin resistance undermines its curative effects on cancer and usually leads to local recurrence. The oxidative stress induced DNA damage repair response is an important mechanism for inducing oxaliplatin resistance by activating autophagy. ELISA is used to detect target genes expression. TMT-based quantitative proteomic analysis was used to investigate the potential mechanisms involved in NORAD interactions based on GO analysis. Transwell assays and apoptosis flow cytometry were used for biological function analysis. CCK-8 was used to calculate IC50 and resistance index (RI) values. Dual-luciferase reporter gene assay, RIP and ChIP assays, and RNA pull-down were used to detect the interaction. Autophagy flux was evaluated using electron microscope and western blotting. Oxidative stress was enhanced by oxaliplatin; and oxaliplatin resistance gastric cancer cell showed lower oxidative stress. TMT labeling showed that NORAD may regulate autophagy flux. NORAD was highly expressed in oxaliplatin-resistant tissues. In vitro experiments indicate that NORAD knockdown decreases the RI (Resistance Index). Oxaliplatin induces oxidative stress and upregulates the expression of NORAD. SGC-7901 shows enhanced oxidative stress than oxaliplatin-resistant cells (SGC-7901-R). NORAD, activated by H3K27ac and CREBBP, enhanced the autophagy flux in SGC-7901-R to suppress the oxidative stress. NORAD binds to miR-433-3p and thereby stabilize the ATG5- ATG12 complex. Our findings illustrate that NORAD, activated by the oxidative stress, can positively regulate ATG5 and ATG12 and enhance the autophagy flux by sponging miR-433-3p. NORAD may be a potential biomarker for predicting oxaliplatin resistance and mediating oxidative stress, and provides therapeutic targets for reversing oxaliplatin resistance.