A proline rich protein from the gingival seal around teeth exhibits antimicrobial properties against Porphyromonas gingivalis

The gingival seal around teeth prevents bacteria from destroying the tooth-supporting tissues and disseminating throughout the body. Porphyromonas gingivalis , a major periodontopathogen, degrades components of the specialized extracellular matrix that mediates attachment of the gingiva to the tooth...

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Bibliographic Details
Published in:Scientific reports 2021-01, Vol.11 (1), p.2353-2353, Article 2353
Main Authors: Fouillen, Aurélien, Mary, Charline, Ponce, Katia Julissa, Moffatt, Pierre, Nanci, Antonio
Format: Article
Language:English
Subjects:
631/326/22
631/326/41
631/45/612
Amino acid sequence
Amino acids
Antimicrobial agents
Bacteria
Biodegradation
Calcium
Electron microscopy
Extracellular matrix
Gingiva
Glutamine
Humanities and Social Sciences
Mammalian cells
multidisciplinary
Porphyromonas gingivalis
Proline
Proteins
Science
Science (multidisciplinary)
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Summary:The gingival seal around teeth prevents bacteria from destroying the tooth-supporting tissues and disseminating throughout the body. Porphyromonas gingivalis , a major periodontopathogen, degrades components of the specialized extracellular matrix that mediates attachment of the gingiva to the tooth. Of these, secretory calcium-binding phosphoprotein proline-glutamine rich 1 (SCPPPQ1) protein has a distinctive resistance to degradation, suggesting that it may offer resistance to bacterial attack. In silico analysis of its amino acid sequence was used to explore its molecular characteristics and to predict its two- and three-dimensional structure. SCPPPQ1 exhibits similarities with both proline-rich and cationic antimicrobial proteins, suggesting a putative antimicrobial potential. A combination of imaging approaches showed that incubation with 20 μM of purified SCPPPQ1 decrease bacterial number (p 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-81791-7