A proline rich protein from the gingival seal around teeth exhibits antimicrobial properties against Porphyromonas gingivalis

The gingival seal around teeth prevents bacteria from destroying the tooth-supporting tissues and disseminating throughout the body. Porphyromonas gingivalis , a major periodontopathogen, degrades components of the specialized extracellular matrix that mediates attachment of the gingiva to the tooth...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2021-01, Vol.11 (1), p.2353-2353, Article 2353
Main Authors: Fouillen, Aurélien, Mary, Charline, Ponce, Katia Julissa, Moffatt, Pierre, Nanci, Antonio
Format: Article
Language:English
Subjects:
631/326/22
631/326/41
631/45/612
Amino acid sequence
Amino acids
Antimicrobial agents
Bacteria
Biodegradation
Calcium
Electron microscopy
Extracellular matrix
Gingiva
Glutamine
Humanities and Social Sciences
Mammalian cells
multidisciplinary
Porphyromonas gingivalis
Proline
Proteins
Science
Science (multidisciplinary)
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c540t-9c4af90cf7afcbe8c2f444c577010776c4ad5db1d77c5504677704062ccddff33
cites cdi_FETCH-LOGICAL-c540t-9c4af90cf7afcbe8c2f444c577010776c4ad5db1d77c5504677704062ccddff33
container_end_page 2353
container_issue 1
container_start_page 2353
container_title Scientific reports
container_volume 11
creator Fouillen, Aurélien
Mary, Charline
Ponce, Katia Julissa
Moffatt, Pierre
Nanci, Antonio
description The gingival seal around teeth prevents bacteria from destroying the tooth-supporting tissues and disseminating throughout the body. Porphyromonas gingivalis , a major periodontopathogen, degrades components of the specialized extracellular matrix that mediates attachment of the gingiva to the tooth. Of these, secretory calcium-binding phosphoprotein proline-glutamine rich 1 (SCPPPQ1) protein has a distinctive resistance to degradation, suggesting that it may offer resistance to bacterial attack. In silico analysis of its amino acid sequence was used to explore its molecular characteristics and to predict its two- and three-dimensional structure. SCPPPQ1 exhibits similarities with both proline-rich and cationic antimicrobial proteins, suggesting a putative antimicrobial potential. A combination of imaging approaches showed that incubation with 20 μM of purified SCPPPQ1 decrease bacterial number (p 
doi_str_mv 10.1038/s41598-021-81791-7
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_3b9c7539470d449683391adf587afdfd</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_3b9c7539470d449683391adf587afdfd</doaj_id><sourcerecordid>2482669024</sourcerecordid><originalsourceid>FETCH-LOGICAL-c540t-9c4af90cf7afcbe8c2f444c577010776c4ad5db1d77c5504677704062ccddff33</originalsourceid><addsrcrecordid>eNp9kklv1DAcxSMEolXpF-CAInHhEvCW2L4gVRVLpUpwgLPleEk8ytiD7anoge_Of5oytBzwwdt7_nl7TfMSo7cYUfGuMNxL0SGCO4G5xB1_0pwSxPqOUEKePuifNOelbBCUnkiG5fPmhNIeMTEMp82vi3aX0xKia3Mw82FQXYitz2nb1tm1U4hTuNFLWxxUOqd9tG11rs6t-zmHMdTS6ljDNpicxgAeQOxcrsGBMOkQS22_prybbwGZoi5HZCgvmmdeL8Wd37dnzfePH75dfu6uv3y6ury47kzPUO2kYdpLZDzX3oxOGOIZY6bnHGHE-QCy7e2ILeemh5sNHBSGBmKMtd5TetZcrVyb9EbtctjqfKuSDupuIuVJaTixWZyiozS8p5JxZBmTg6BUYm19L2Bz6y2w3q-s3X7cOmtcrFkvj6CPlRhmNaUbxQVDEmEAvLkH5PRj70pV21CMWxYdXdoXRZggwyARYWB9_Y91k_Y5wlMdXEhKKcQALrK64AdKyc4fD4OROoRFrWFREBZ1FxbFYdGrh9c4LvkTDTDQ1VBAipPLf_f-D_Y3oGDNhA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2480999886</pqid></control><display><type>article</type><title>A proline rich protein from the gingival seal around teeth exhibits antimicrobial properties against Porphyromonas gingivalis</title><source>Open Access: PubMed Central</source><source>ProQuest - Publicly Available Content Database</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature - nature.com Journals - Fully Open Access</source><creator>Fouillen, Aurélien ; Mary, Charline ; Ponce, Katia Julissa ; Moffatt, Pierre ; Nanci, Antonio</creator><creatorcontrib>Fouillen, Aurélien ; Mary, Charline ; Ponce, Katia Julissa ; Moffatt, Pierre ; Nanci, Antonio</creatorcontrib><description>The gingival seal around teeth prevents bacteria from destroying the tooth-supporting tissues and disseminating throughout the body. Porphyromonas gingivalis , a major periodontopathogen, degrades components of the specialized extracellular matrix that mediates attachment of the gingiva to the tooth. Of these, secretory calcium-binding phosphoprotein proline-glutamine rich 1 (SCPPPQ1) protein has a distinctive resistance to degradation, suggesting that it may offer resistance to bacterial attack. In silico analysis of its amino acid sequence was used to explore its molecular characteristics and to predict its two- and three-dimensional structure. SCPPPQ1 exhibits similarities with both proline-rich and cationic antimicrobial proteins, suggesting a putative antimicrobial potential. A combination of imaging approaches showed that incubation with 20 μM of purified SCPPPQ1 decrease bacterial number (p &lt; 0.01). Fluorescence intensity decreased by 70% following a 2 h incubation of Porphyromonas gingivalis with the protein. Electron microscopy analyses revealed that SCPPPQ1 induced bacterial membrane disruption and breaches. While SCPPPQ1 has no effect on mammalian cells, our results suggest that it is bactericidal to Porphyromonas gingivalis, and that this protein, normally present in the gingival seal, may be exploited to maintain a healthy seal and prevent systemic dissemination of bacteria.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-021-81791-7</identifier><identifier>PMID: 33504866</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/326/22 ; 631/326/41 ; 631/45/612 ; Amino acid sequence ; Amino acids ; Antimicrobial agents ; Bacteria ; Biodegradation ; Calcium ; Electron microscopy ; Extracellular matrix ; Gingiva ; Glutamine ; Humanities and Social Sciences ; Mammalian cells ; multidisciplinary ; Porphyromonas gingivalis ; Proline ; Proteins ; Science ; Science (multidisciplinary)</subject><ispartof>Scientific reports, 2021-01, Vol.11 (1), p.2353-2353, Article 2353</ispartof><rights>The Author(s) 2021</rights><rights>The Author(s) 2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-9c4af90cf7afcbe8c2f444c577010776c4ad5db1d77c5504677704062ccddff33</citedby><cites>FETCH-LOGICAL-c540t-9c4af90cf7afcbe8c2f444c577010776c4ad5db1d77c5504677704062ccddff33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2480999886/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2480999886?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33504866$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fouillen, Aurélien</creatorcontrib><creatorcontrib>Mary, Charline</creatorcontrib><creatorcontrib>Ponce, Katia Julissa</creatorcontrib><creatorcontrib>Moffatt, Pierre</creatorcontrib><creatorcontrib>Nanci, Antonio</creatorcontrib><title>A proline rich protein from the gingival seal around teeth exhibits antimicrobial properties against Porphyromonas gingivalis</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>The gingival seal around teeth prevents bacteria from destroying the tooth-supporting tissues and disseminating throughout the body. Porphyromonas gingivalis , a major periodontopathogen, degrades components of the specialized extracellular matrix that mediates attachment of the gingiva to the tooth. Of these, secretory calcium-binding phosphoprotein proline-glutamine rich 1 (SCPPPQ1) protein has a distinctive resistance to degradation, suggesting that it may offer resistance to bacterial attack. In silico analysis of its amino acid sequence was used to explore its molecular characteristics and to predict its two- and three-dimensional structure. SCPPPQ1 exhibits similarities with both proline-rich and cationic antimicrobial proteins, suggesting a putative antimicrobial potential. A combination of imaging approaches showed that incubation with 20 μM of purified SCPPPQ1 decrease bacterial number (p &lt; 0.01). Fluorescence intensity decreased by 70% following a 2 h incubation of Porphyromonas gingivalis with the protein. Electron microscopy analyses revealed that SCPPPQ1 induced bacterial membrane disruption and breaches. While SCPPPQ1 has no effect on mammalian cells, our results suggest that it is bactericidal to Porphyromonas gingivalis, and that this protein, normally present in the gingival seal, may be exploited to maintain a healthy seal and prevent systemic dissemination of bacteria.</description><subject>631/326/22</subject><subject>631/326/41</subject><subject>631/45/612</subject><subject>Amino acid sequence</subject><subject>Amino acids</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Biodegradation</subject><subject>Calcium</subject><subject>Electron microscopy</subject><subject>Extracellular matrix</subject><subject>Gingiva</subject><subject>Glutamine</subject><subject>Humanities and Social Sciences</subject><subject>Mammalian cells</subject><subject>multidisciplinary</subject><subject>Porphyromonas gingivalis</subject><subject>Proline</subject><subject>Proteins</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp9kklv1DAcxSMEolXpF-CAInHhEvCW2L4gVRVLpUpwgLPleEk8ytiD7anoge_Of5oytBzwwdt7_nl7TfMSo7cYUfGuMNxL0SGCO4G5xB1_0pwSxPqOUEKePuifNOelbBCUnkiG5fPmhNIeMTEMp82vi3aX0xKia3Mw82FQXYitz2nb1tm1U4hTuNFLWxxUOqd9tG11rs6t-zmHMdTS6ljDNpicxgAeQOxcrsGBMOkQS22_prybbwGZoi5HZCgvmmdeL8Wd37dnzfePH75dfu6uv3y6ury47kzPUO2kYdpLZDzX3oxOGOIZY6bnHGHE-QCy7e2ILeemh5sNHBSGBmKMtd5TetZcrVyb9EbtctjqfKuSDupuIuVJaTixWZyiozS8p5JxZBmTg6BUYm19L2Bz6y2w3q-s3X7cOmtcrFkvj6CPlRhmNaUbxQVDEmEAvLkH5PRj70pV21CMWxYdXdoXRZggwyARYWB9_Y91k_Y5wlMdXEhKKcQALrK64AdKyc4fD4OROoRFrWFREBZ1FxbFYdGrh9c4LvkTDTDQ1VBAipPLf_f-D_Y3oGDNhA</recordid><startdate>20210127</startdate><enddate>20210127</enddate><creator>Fouillen, Aurélien</creator><creator>Mary, Charline</creator><creator>Ponce, Katia Julissa</creator><creator>Moffatt, Pierre</creator><creator>Nanci, Antonio</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><general>Nature Portfolio</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20210127</creationdate><title>A proline rich protein from the gingival seal around teeth exhibits antimicrobial properties against Porphyromonas gingivalis</title><author>Fouillen, Aurélien ; Mary, Charline ; Ponce, Katia Julissa ; Moffatt, Pierre ; Nanci, Antonio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-9c4af90cf7afcbe8c2f444c577010776c4ad5db1d77c5504677704062ccddff33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>631/326/22</topic><topic>631/326/41</topic><topic>631/45/612</topic><topic>Amino acid sequence</topic><topic>Amino acids</topic><topic>Antimicrobial agents</topic><topic>Bacteria</topic><topic>Biodegradation</topic><topic>Calcium</topic><topic>Electron microscopy</topic><topic>Extracellular matrix</topic><topic>Gingiva</topic><topic>Glutamine</topic><topic>Humanities and Social Sciences</topic><topic>Mammalian cells</topic><topic>multidisciplinary</topic><topic>Porphyromonas gingivalis</topic><topic>Proline</topic><topic>Proteins</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fouillen, Aurélien</creatorcontrib><creatorcontrib>Mary, Charline</creatorcontrib><creatorcontrib>Ponce, Katia Julissa</creatorcontrib><creatorcontrib>Moffatt, Pierre</creatorcontrib><creatorcontrib>Nanci, Antonio</creatorcontrib><collection>SpringerOpen(OpenAccess)</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest - Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fouillen, Aurélien</au><au>Mary, Charline</au><au>Ponce, Katia Julissa</au><au>Moffatt, Pierre</au><au>Nanci, Antonio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A proline rich protein from the gingival seal around teeth exhibits antimicrobial properties against Porphyromonas gingivalis</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2021-01-27</date><risdate>2021</risdate><volume>11</volume><issue>1</issue><spage>2353</spage><epage>2353</epage><pages>2353-2353</pages><artnum>2353</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>The gingival seal around teeth prevents bacteria from destroying the tooth-supporting tissues and disseminating throughout the body. Porphyromonas gingivalis , a major periodontopathogen, degrades components of the specialized extracellular matrix that mediates attachment of the gingiva to the tooth. Of these, secretory calcium-binding phosphoprotein proline-glutamine rich 1 (SCPPPQ1) protein has a distinctive resistance to degradation, suggesting that it may offer resistance to bacterial attack. In silico analysis of its amino acid sequence was used to explore its molecular characteristics and to predict its two- and three-dimensional structure. SCPPPQ1 exhibits similarities with both proline-rich and cationic antimicrobial proteins, suggesting a putative antimicrobial potential. A combination of imaging approaches showed that incubation with 20 μM of purified SCPPPQ1 decrease bacterial number (p &lt; 0.01). Fluorescence intensity decreased by 70% following a 2 h incubation of Porphyromonas gingivalis with the protein. Electron microscopy analyses revealed that SCPPPQ1 induced bacterial membrane disruption and breaches. While SCPPPQ1 has no effect on mammalian cells, our results suggest that it is bactericidal to Porphyromonas gingivalis, and that this protein, normally present in the gingival seal, may be exploited to maintain a healthy seal and prevent systemic dissemination of bacteria.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>33504866</pmid><doi>10.1038/s41598-021-81791-7</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2045-2322
ispartof Scientific reports, 2021-01, Vol.11 (1), p.2353-2353, Article 2353
issn 2045-2322
2045-2322
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_3b9c7539470d449683391adf587afdfd
source Open Access: PubMed Central; ProQuest - Publicly Available Content Database; Free Full-Text Journals in Chemistry; Springer Nature - nature.com Journals - Fully Open Access
subjects 631/326/22
631/326/41
631/45/612
Amino acid sequence
Amino acids
Antimicrobial agents
Bacteria
Biodegradation
Calcium
Electron microscopy
Extracellular matrix
Gingiva
Glutamine
Humanities and Social Sciences
Mammalian cells
multidisciplinary
Porphyromonas gingivalis
Proline
Proteins
Science
Science (multidisciplinary)
title A proline rich protein from the gingival seal around teeth exhibits antimicrobial properties against Porphyromonas gingivalis
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T09%3A57%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20proline%20rich%20protein%20from%20the%20gingival%20seal%20around%20teeth%20exhibits%20antimicrobial%20properties%20against%20Porphyromonas%20gingivalis&rft.jtitle=Scientific%20reports&rft.au=Fouillen,%20Aur%C3%A9lien&rft.date=2021-01-27&rft.volume=11&rft.issue=1&rft.spage=2353&rft.epage=2353&rft.pages=2353-2353&rft.artnum=2353&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-021-81791-7&rft_dat=%3Cproquest_doaj_%3E2482669024%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c540t-9c4af90cf7afcbe8c2f444c577010776c4ad5db1d77c5504677704062ccddff33%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2480999886&rft_id=info:pmid/33504866&rfr_iscdi=true