The Pepper MAP Kinase CaAIMK1 Positively Regulates ABA and Drought Stress Responses

Protein phosphorylation and dephosphorylation are important mechanisms that regulate many cellular processes. Protein kinases usually function in the regulation of the stress responses by adjusting activity via phosphorylation of target proteins. Here, we isolated CaAIMK1 ( Capsicum annuum ABA Induc...

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Bibliographic Details
Published in:Frontiers in plant science 2020-05, Vol.11, p.720-720
Main Authors: Jeong, Soongon, Lim, Chae Woo, Lee, Sung Chul
Format: Article
Language:English
Subjects:
ABA
drought
kinase
phosphorylation
Plant Science
stomata
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Summary:Protein phosphorylation and dephosphorylation are important mechanisms that regulate many cellular processes. Protein kinases usually function in the regulation of the stress responses by adjusting activity via phosphorylation of target proteins. Here, we isolated CaAIMK1 ( Capsicum annuum ABA Induced MAP Kinase 1) from the pepper leaves that had been subjected to drought stress. CaAIMK1 transcripts were induced by drought, abscisic acid (ABA), high salinity, and H 2 O 2 ; further, the CaAIMK1-Green fluorescent protein localized in the nucleus and cytoplasm. We performed genetic studies using CaAIMK1 -silenced pepper plants and CaAIMK1- overexpressing (OX) Arabidopsis plants. CaAIMK1- silenced pepper plants showed a drought-sensitive phenotype characterized by altered ABA signaling, including low leaf temperatures, and large stomatal apertures. CaAIMK1- OX plants exhibited a contrasting drought-tolerant phenotype characterized by decreased levels of transpirational water loss and increased expression levels of Arabidopsis stress-related genes. In CaAIMK1 K32N -OX transgenic Arabidopsis plants, sensitivity to ABA and drought was restored. Collectively, these results demonstrate that CaAIMK1 positively regulates the drought stress responses via an ABA-dependent pathway.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2020.00720