Dental pulp cells cocultured with macrophages aggravate the inflammatory conditions stimulated by LPS

• Published in: BMC oral health 2023-12, Vol.23 (1), p.991-991, Article 991
• Main Authors: Wang, Min-Ching, Chang, Kuo-Wei, Lin, Shu-Chun, Hsu, Ling-Hsin, Hung, Pei-Shih
• Format: Article
• Language: English
• Subjects: Antibiotics; Cell growth; Cell viability; Coculture; Cytokines; Cytology; Dental pulp; Dental pulp cells (DPCs); Health aspects; Immunoassay; Inflammation; Interleukin 6; Interleukin 8; Lipopolysaccharides; LPS; Macrophage; Macrophages; Medical prognosis; RNA; Scientific equipment and supplies industry; Statistical analysis; Thermal cycling
• ISSN: 1472-6831; 1472-6831
• DOI: 10.1186/s12903-023-03625-4

Pulp inflammation is complex interactions between different types of cells and cytokines. To mimic the interactions of different types of cells in inflamed dental pulp tissues, dental pulp cells (DPCs) were cocultured with different ratios of macrophages (THP-1) or LPS treatment. DPCs were cocultured with various ratios of THP-1, then photographed cell morphology and determined cell viability by MTT assay at preset times. Total RNA was also extracted to measure the inflammation marker-IL-6 and IL-8 expressions by RT-Q-PCR. The DPCs and THP-1 were treated with 0.01 - 1μg/ml lipopolysaccharide (LPS) and extract RNA at preset times, and detected IL-6 and IL-8 expression. DPCs were cocultured with various ratios of THP-1 with 0.1 μg/mL LPS, and detected IL-6 and IL-8 expression after 24 and 48 h. The data were analyzed by unpaired t-test or Mann-Whitney test. Differences were considered statistically significant when p