Oxidative stress in the latissimus dorsi muscle of diabetic rats

The purpose of the present study was to investigate the effects of experimental diabetes on the oxidant and antioxidant status of latissimus dorsi (LD) muscles of male Wistar rats (220 +/- 5 g, N = 11). Short-term (5 days) diabetes was induced by a single injection of streptozotocin (STZ, 50 mg/kg,...

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Published in:Brazilian journal of medical and biological research 2000-11, Vol.33 (11), p.1363-1368
Main Authors: De Angelis, K L, Cestari, I A, Barp, J, Dall'Ago, P, Fernandes, T G, de Bittencourt, P I, Belló-Klein, A, Belló, A A, Llesuy, S, Irigoyen, M C
Format: Article
Language:English
Subjects:
Animals
BIOLOGY
catalase
Diabetes Mellitus, Experimental - metabolism
glutathione
latissimus dorsi muscle
Linear Models
Luminescent Measurements
Male
MEDICINE, RESEARCH & EXPERIMENTAL
Muscle, Skeletal - metabolism
oxidative stress
Oxidative Stress - physiology
Rats
Rats, Wistar
streptozotocin-diabetes
Thiobarbituric Acid Reactive Substances - metabolism
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Summary:The purpose of the present study was to investigate the effects of experimental diabetes on the oxidant and antioxidant status of latissimus dorsi (LD) muscles of male Wistar rats (220 +/- 5 g, N = 11). Short-term (5 days) diabetes was induced by a single injection of streptozotocin (STZ, 50 mg/kg, iv; glycemia >300 mg/dl). LD muscle of STZ-diabetic rats presented higher levels of thiobarbituric acid reactive substances (TBARS) and chemiluminescence (0.36 +/- 0.02 nmol/mg protein and 14706 +/- 1581 cps/mg protein) than LD muscle of normal rats (0.23 +/- 0.04 nmol/mg protein and 7389 +/- 1355 cps/mg protein). Diabetes induced a 92% increase in catalase and a 27% increase in glutathione S-transferase activities in LD muscle. Glutathione peroxidase activity was reduced (58%) in STZ-diabetic rats and superoxide dismutase activity was similar in LD muscle of both groups. A positive correlation was obtained between catalase activity and the oxidative stress of LD, as evaluated in terms of TBARS (r = 0.78) and by chemiluminescence (r = 0.89). Catalase activity also correlated inversely with glutathione peroxidase activity (r = 0.79). These data suggest that an increased oxidative stress in LD muscle of diabetic rats may be related to skeletal muscle myopathy.
ISSN:0100-879X
1414-431X
0100-879X
1414-431X
DOI:10.1590/S0100-879X2000001100016